Ligands that bind tgf-beta receptor ii

ABSTRACT

The disclosure provides an anti-TGFbetaRII immunoglobulin single variable domain. Suitably, an anti-TGFbetaRII immunoglobulin single variable domain in accordance with the disclosure is one having an amino acid sequence as set forth in any one of SEQ ID NO:1-28 having up to 5 amino acid substitutions, deletions or additions. The disclosure further provides a polypeptide and pharmaceutical composition for treating a disease associated with TGFbeta signalling and suitably a disease selected from the group of: tissue fibrosis, such as pulmonary fibrosis, including idiopathic pulmonary fibrosis; liver fibrosis, including cirrhosis and chronic hepatitis; rheumatoid arthritis; ocular disorders; fibrosis of the skin, including keloid of skin; Dupuytren&#39;s Contracture; kidney fibrosis such as nephritis and nephrosclerosis; wound healing; scarring reduction; and a vascular condition, such as restenosis.

BACKGROUND

Transforming Growth Factor-β (TFGbeta; TGFβ (TGF-β) is a signaling molecule that mediates signal transduction into cells through binding to a TGFbeta receptor (TGFbetaR; TGFβR (TGF-βR)). TGFbeta signaling activity regulates cell differentiation and growth, the nature of its effect, i.e. as cell growth-promoter, growth-suppressor or inducer of other cell functions, being dependent on cell type (see Roberts, et al., The transforming growth factor-betas, Peptide Growth Factors and Their Receptors, Part I, ed. by Sporn, M. B. & Roberts, A. B., Springer-Verlag, Berlin, 1990, p. 419-472).

TGFbeta is produced by a wide variety of cell types, and its cognate receptors are expressed in a wide variety of organs and cells (see Shi and Massague, Cell, Volume 113, Issue 6, 13 Jun. 2003, Pages 685-700; Biol. Signals., Vol. 5, p. 232, 1996 and Pulmonary Fibrosis, Vol. 80 of Lung Biology in Health and Disease Series, ed. by Phan, et al., p. 627, Dekker, New York, 1995). TGFbeta receptors have been identified to fall into three types: TGFbetaRI (TGFβRI) (TGFbeta type I receptor (Franzen et al., Cell, Vol. 75, No. 4, p. 681, 1993; GenBank Accession No: L11695)); TGFbetaRII (TGFβRII) (TGFbeta type II receptor (Herbert et al., Cell, Vol. 68, No. 4, p. 775, 1992; GenBank Accession No: M85079)) and TGFbetaRIII (TGFbeta type III receptor (Lopez-Casillas, Cell, Vol. 67, No. 4, p. 785, 1991; GenBank Accession No: L07594)). TGFbetaRI and TGFbetaRII have been shown to be essential for the signal transduction of TGF-beta (Laiho et al., J. Biol. Chem., Vol. 265, p. 18518, 1990 and Laiho et al., J. Biol. Chem., Vol. 266, p. 9108, 1991), while TGFbetaRIII is not thought to be essential.

TGFbeta signaling is mediated through its binding to both TGFbetaRI and RII. When the ligand binds to the extracellular ligand binding domain, the two receptors are brought together, allowing RII to phosphorylate RI and begin the signaling cascade through the phosphorylation of Smad proteins (see Shi and Massague as referred to above).

Three isoforms of TGFbeta have been identified in mammals: TGFbeta1, TGFbeta2, and TGFbeta3. Each isoform is multifunctional and acts in self-regulatory feedback mechanisms to control bioavailability for developmental processes and to maintain tissue homeostasis (as reviewed in ten Dijke and Arthur, Nature Reviews, Molecular Cell Biology, Vol. 8, November 2007, p. 857-869). Levels of TFGbeta are controlled by regulation through TGFbeta expression as well as through binding to proteoglycan, i.e., the extracellular matrix (ECM).

Dysregulated TGFbeta signaling, such as excess TGFbeta signaling and high levels of bioavailable TGFbeta, is implicated in a number of pathologies, including fibroses of various tissues, such as pulmonary fibrosis and cirrhosis, chronic hepatitis, rheumatoid arthritis, ocular disorders, vascular restenosis, keloid of skin, and the onset of nephrosclerosis.

Accordingly, there is a need to provide compounds that block or disrupt TGFbeta signaling in a specific manner, such as through binding to the TGFbeta receptor II. Such compounds can be used in therapeutics.

SUMMARY

The disclosure relates to an anti-TGFbetaRII immunoglobulin single variable domain. Suitably, an anti-TGFbetaRII immunoglobulin single variable domain in accordance with the disclosure is one which binds to TGFbetaRII with an equilibrium dissociation constant (KD) in the range of 10 pM to 50 nM, optionally 10 pM to 10 nM, optionally 100 pM to 10 nM. In one embodiment, the anti-TGFbetaRII immunoglobulin single variable domain is one which binds TGFbetaRII with high affinity (high potency) and has an equilibrium dissociation constant of 10 pM to 500 pM. In one embodiment, the anti-TGFbetaRII immunoglobulin single variable domain is one which binds TGFbetaRII with an affinity (KD) of approximately 100 pM. In one embodiment, the anti-TGFbetaRII immunoglobulin single variable domain is one which binds TGFbetaRII with an affinity (KD) of less than 100 pM. In another embodiment, the anti-TGFbetaRII immunoglobulin single variable domain is one which binds TGFbetaRII with moderate affinity (low potency) and has an equilibrium dissociation constant of 500 pM to 50 nM, preferably 500 pM to 10 nM. In another aspect, the disclosure provides an isolated polypeptide comprising an anti-TGFbetaRII immunoglobulin single variable domain. Suitably, the isolated polypeptide binds to human TGFbetaRII. In another embodiment, the isolated polypeptide also binds to TGFbetaRII derived from a different species such as mouse, dog or monkeys, such as cynomolgus monkeys (cyno). Suitably, the isolated polypeptide binds to both mouse and human TGFbetaRII. Such cross reactivity between TGFbetaRII from humans and other species allows the same antibody construct to be used in an animal disease model, as well as in humans.

In an aspect of the disclosure there is provided an anti-TGFbetaRII immunoglobulin single variable domain having an amino acid sequence as set forth in any one of SEQ ID NO:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285 and 287, and having up to 5 amino acid alterations, wherein each amino acid alteration is an amino acid substitution, deletion or addition i.e. up to 5 amino acid substitutions, deletions or additions, in any combination. In a particular embodiment the amino acid substitutions are conservative substitutions.

In an embodiment, the anti-TGFbetaRII immunoglobulin single variable domain has the amino acid sequence as set forth in SEQ ID NO: 234 or 279 and having up to 5 amino acid alterations, wherein each amino acid alteration is a an amino acid substitution, deletion or addition. In a particular embodiment, the amino acid alteration(s) are not within CDR3, more specifically not within CDR3 and CDR1, or CDR3 and CDR2, more specifically not within any of the CDRs. In an embodiment, the anti-TGFbetaRII immunoglobulin single variable domain consists of any one of the following sequences: SEQ ID NO:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285 and 287. In an embodiment the anti-TGFbetaRII immunoglobulin single variable domain consists of an amino acid sequence of SEQ ID NO: 234 or 236.

It is not intended to cover any specific anti-TGFbetaRII immunoglobulin single variable domain sequence disclosed in WO 2011012609. For the avoidance of doubt each and every sequence disclosed in WO 2011012609 may be disclaimed from the present invention. In particular DOM23h-271 (SEQ ID NO:4) and DOM-23h-439 (SEQ ID NO:10) as disclosed in WO 2011012609 may be disclaimed. An anti-TGFbetaRII immunoglobulin single variable domain consisting of the amino acid sequence as set forth in SEQ ID NO: 199 or 201 herein may be disclaimed.

An anti-TGFbetaRII immunoglobulin single variable domain according to the invention may comprise one or more (e.g. 1, 2, 3, 4, or 5) C-terminal alanine residues. Alternatively, an anti-TGFbetaRII immunoglobulin single variable domain may comprise a C-terminal peptide of up to 5 amino acids in length. In an embodiment, the C-terminal peptide comprises 1, 2, 3, 4, or 5 amino acids.

A person skilled in the art is able to deduce from a given single variable domain sequence, e.g. one having a sequence as set out in any one of SEQ ID NO:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285 and 287 which CDR sequences are contained within them using the various methods outlined herein e.g. CDR sequences as defined by reference to Kabat (1987), Chothia (1989), AbM or contact methods, or a combination of these methods. Suitably, CDR sequences are determined using the method of Kabat described herein. In one embodiment, the CDR sequences of each sequence are those set out in tables 1, 2, 9, and 13.

In an aspect of the invention an anti-TGFbetaRII immunoglobulin single variable domain of the disclosure has 90% or greater than 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1-28.

In an aspect of the invention an anti-TGFbetaRII immunoglobulin single variable domain of the disclosure has an amino acid sequence selected from the group consisting of SEQ ID NO:1-28 with 25 or fewer amino acid changes. In a particular embodiment an anti-TGFbetaRII immunoglobulin single variable domain of the disclosure has an amino acid sequence selected from the group consisting of SEQ ID NO:1-28 with 20 or fewer, 15 or fewer, 10 or fewer, 9 or fewer, 8 or fewer, 7 or fewer, 6 or fewer, or 5 or fewer amino acid changes.

In an aspect of the disclosure there is provided an isolated polypeptide comprising an anti-TGFbetaRII immunoglobulin single variable domain of the disclosure, in particular an anti-TGFbetaRII immunoglobulin single variable domain identical to an amino acid sequence selected from the group consisting of SEQ ID NO:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285 and 287 wherein said isolated polypeptide binds to TGFbetaRII.

In an aspect of the disclosure there is provided an isolated polypeptide encoded by a nucleotide sequence that is at least 80% identical to at least one nucleic acid sequence selected from the group of: SEQ ID NOS:39-66, wherein said isolated polypeptide binds to TGFbetaRII.

An anti-TGFbetaRII immunoglobulin single variable domain or a polypeptide in accordance with any aspect of the disclosure may comprise any of the following amino acids: R at position 39, I at position 48, D at position 53, N at position 61, R at position 61, K at position 61, R at position 64, F at position 64, D at position 64, E at position 64, Y at position 64, H at position 102, or S at position 103 of the immunoglobulin single variable domain, said position being according to the kabat numbering convention. In one embodiment, the immunoglobulin single variable domain or polypeptide comprises a combination of these amino acids. In another embodiment, the immunoglobulin single variable domain or polypeptide comprises amino acid N at 61 and R at 64. In another embodiment, the immunoglobulin single variable domain or polypeptide comprises amino acid R or K at position 61. In an embodiment, the anti-TGFbetaRII immunoglobulin single variable domain comprises an I at position 48 in addition to any one of the aforementioned residues at position 61 and/or 64. In these embodiments, the amino acid numbering is that of the immunoglobulin single variable domain, as exemplified, for example, by those sequences given in SEQ ID NOs:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285 and 287.

An anti-TGFbetaRII immunoglobulin single variable domain or a polypeptide in accordance with any aspect of the disclosure may comprise one of the following amino acid combinations selected from the group: N at position 61 and R at position 64; R at position 61 and E at position 64; R at position 61 and M at position 64; R at position 61 and F at position 64; R at position 61 and Y at position 64; and R at position 61 and D at position 64 of the immunoglobulin single variable domain. In an embodiment, the anti-TGFbetaRII immunoglobulin single variable domain comprises an I at position 48 in addition to any one of the aforementioned combination of residues at positions 61 and 64.

In another aspect, there is provided a ligand or binding moiety that has binding specificity for TGFbetaRII and inhibits the binding of an anti-TGFbetaRII immunoglobulin single variable domain comprising an amino acid sequence selected from the group of SEQ ID NOs:1-28 to TGFbetaRII.

In a further aspect of the disclosure, there is provided a fusion protein comprising an immunoglobulin single variable domain, polypeptide or ligand in accordance with any aspect of the disclosure.

In one embodiment, the immunoglobulin single variable domain, polypeptide, ligand or fusion protein in accordance with the disclosure is one which neutralises TGFbeta activity. Suitably, the immunoglobulin single variable domain or polypeptide in accordance with the disclosure inhibits binding of TGFbeta to TGFbetaRII. In another embodiment, the immunoglobulin single variable domain or polypeptide in accordance with the disclosure inhibits TGFbeta signalling activity through TGFbetaRII. In another embodiment, the immunoglobulin single variable domain or polypeptide in accordance with the disclosure suppresses TGFbeta activity, in particular, TGFbeta cell growth activity and/or fibrogenic activity. Suitably, TGFbetaRII is human TGFbetaRII.

In one embodiment, the immunoglobulin single variable domain, polypeptide, ligand or fusion protein in accordance with the disclosure is devoid of TGFbetaRII agonist activity at 15 micromolar (μM).

In another aspect, there is provided an immunoglobulin single variable domain, polypeptide, ligand or fusion protein in accordance with any aspect of the disclosure further comprising a half-life extending moiety. Suitably, the half-life extending moiety is a polyethylene glycol moiety, serum albumin or a fragment thereof, transferrin receptor or a transferrin-binding portion thereof, or an antibody or antibody fragment comprising a binding site for a polypeptide that enhances half-life in vivo. In one embodiment, the half-life extending moiety is an antibody or antibody fragment comprising a binding site for serum albumin or neonatal Fc receptor. In another embodiment, the half-life extending moiety is a dAb, antibody or antibody fragment.

In another aspect, the disclosure provides an isolated or recombinant nucleic acid encoding a polypeptide comprising an anti-TGFbetaRII immunoglobulin single variable domain, polypeptide, ligand or fusion protein in accordance with any aspect of the disclosure.

In one embodiment, the isolated or recombinant nucleic acid molecule comprises or consists of a nucleic acid molecule selected from the group of any of the nucleic acid molecules having the sequences set out in SEQ ID NOS:39-76, 203, 205, 207, 212, 233, 235, 237, 239, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286.

In one aspect, the disclosure provides an isolated or recombinant nucleic acid, wherein the nucleic acid comprises a nucleotide sequence that is at least 80% identical to the nucleotide sequence of any of the nucleic acid molecules having the sequences set out in SEQ ID NOS:39-66, and wherein the nucleic acid encodes a polypeptide comprising an immunoglobulin single variable domain that specifically binds to TGFbetaRII.

In another aspect, there is provided a vector comprising a nucleic acid in accordance with the disclosure.

In a further aspect, there is provided a host cell comprising a nucleic acid or a vector in accordance with the disclosure. In yet another aspect of the disclosure there is provided a method of producing a polypeptide comprising an anti-TGFbetaRII immunoglobulin single variable domain or a polypeptide or ligand or a fusion protein in accordance with the disclosure, the method comprising maintaining a host cell in accordance with the disclosure under conditions suitable for expression of said nucleic acid or vector, whereby a polypeptide comprising an immunoglobulin single variable domain, polypeptide or ligand or fusion protein is produced. Optionally, the method further comprises the step of isolating the polypeptide and optionally producing a variant, e.g., a mutated variant, having an improved affinity (Kd); or EC50 for TGFbeta neutralization in a standard assay than the isolated polypeptide. Suitable assays for TGFbeta activity, such as a cell sensor assay, are described herein, for example, in the Examples section.

In one aspect of the disclosure, the anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or ligand or fusion protein in accordance with the disclosure is for use as a medicament. Accordingly, there is provided a composition comprising anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or ligand or fusion protein in accordance with the disclosure for use as a medicament.

In one aspect of the disclosure, there is provided a use of an anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or ligand or fusion protein in accordance with the disclosure for the manufacture of a medicament, particularly for use in treating disease associated with TGFbeta signalling.

Suitably, the anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or ligand or fusion protein or composition in accordance with the disclosure is for treatment of a disease associated with TGFbeta signaling. Suitably, the disease is a tissue fibrosis, such as pulmonary fibrosis including idiopathic pulmonary fibrosis; liver fibrosis, including cirrhosis and chronic hepatitis; rheumatoid arthritis; ocular disorders; or fibrosis of the skin including keloid of skin; Dupuytren's Contracture; and kidney fibrosis such as nephritis and nephrosclerosis; or a vascular condition such as restenosis. Other diseases associated with TGFbeta signaling include vascular diseases such as hypertension, pre-eclampsia, hereditary haemorrhagic telangtiectasia type I (HHT1), HHT2, pulmonary arterial hypertension, aortic aneurysms, Marfan syndrome, familial aneurysm disorder, Loeys-Dietz syndrome, arterial tortuosity syndrome (ATS). Other diseases associated with TGFbeta signaling include diseases of the musculoskeletal system, such as Duchenne's muscular dystrophy and muscle fibrosis. Further diseases associated with TGFbeta signaling include cancer, such as colon, gastric, and pancreatic cancer, as well as glioma and NSCLC. In addition, the disclosure provides methods for targeting cancer by modulating TGFbeta signaling in tumour angiogenesis. Other diseases or conditions include those related to tissue scarring. Other diseases include pulmonary diseases such as COPD (Chronic obstructive pulmonary disease). An anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or ligand or fusion protein or composition in accordance with the disclosure may be used in wound healing and/or to prevent or improve the formation of scars. In one aspect, the disclosure provides the anti-TGFbetaRII single variable domain, ligand or antagonist, composition or fusion protein for intradermal delivery. In one aspect, the disclosure provides the anti-TGFbetaRII single variable domain, ligand or antagonist or fusion protein for delivery to the skin of a patient. In one aspect, the disclosure provides the use of the anti-TGFbetaRII single variable domain, ligand or antagonist or fusion protein in the manufacture of a medicament for intradermal delivery. In one aspect, the disclosure provides the use of the anti-TGFbetaRII single variable domain or antagonist or fusion protein in accordance with the disclosure in the manufacture of a medicament for delivery to the skin of a patient.

In one embodiment, the variable domain is substantially monomeric. In a particular embodiment the variable domain is 65%-98% monomeric in solution as determined by SEC-MALS. In another embodiment the variable domain is 65%-100%, 70%-100%, 75%-100%, 80%-100%, 85%-100%, 90%-100%, 95%-100% monomeric in solution as determined by SEC-MALS.

In another embodiment, the variable domain, ligand, fusion protein or polypeptide as disclosed herein, particularly when in a pharmaceutical composition, does not contain any one or combination or all of the following post-translational modifications: deamidation, oxidation or glycosylation. In a particular embodiment the variable domain, ligand, fusion protein or polypeptide according to the disclosure does not deamidate.

Suitably, the composition is for therapy or prophylaxis of a TGFbeta-mediated condition in a human.

Accordingly, in one embodiment, there is provided an anti-TGFbetaRII dAb for treating fibrosis of the skin, in particular keloid disease or Dupuytren's Contracture. Suitably, the anti-TGFbetaRII dAb is provided as a substantially monomeric dAb for intradermal delivery, preferably lacking any tag (i.e., untagged) such as a myc or another purification tag.

In one aspect, the composition is a pharmaceutical composition and further comprises a pharmaceutically acceptable carrier, excipient or diluent.

In another aspect, there is provided a method of treating and/or preventing an TGFbeta-mediated condition in a human patient, the method comprising administering a composition comprising an anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or ligand in accordance with the disclosure the to the patient.

In a further aspect, the disclosure provides an intradermal delivery device containing a composition in accordance with the disclosure. Suitably, such a device is a microneedle or collection of microneedles.

An a further aspect, there is provided a kit comprising an anti-TGFbetaRII single variable domain or polypeptide as disclosed herein and a device, such as an intradermal delivery device, for applying said single variable domain or polypeptide to the skin.

DETAILED DESCRIPTION

Within this specification, the disclosure has been described, with reference to embodiments, in a way which enables a clear and concise specification to be written. It is intended and should be appreciated that embodiments may be variously combined or separated without parting from the disclosure.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art (e.g., in cell culture, molecular genetics, nucleic acid chemistry, hybridization techniques and biochemistry). Standard techniques are used for molecular, genetic and biochemical methods (see generally, Sambrook, et al., Molecular Cloning: A Laboratory Manual, 2d ed. (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. and Ausubel, et al., Short Protocols in Molecular Biology (1999) 4th Ed, John Wiley & Sons, Inc., which are incorporated herein by reference) and chemical methods.

Immunoglobulin: As used herein, “immunoglobulin” refers to a family of polypeptides which retain the immunoglobulin fold characteristic of antibody molecules, which contain two 13 sheets and, usually, a conserved disulphide bond.

Domain: As used herein “domain” refers to a folded protein structure which retains its tertiary structure independently of the rest of the protein. Generally, domains are responsible for discrete functional properties of proteins and in many cases may be added, removed or transferred to other proteins without loss of function of the remainder of the protein and/or of the domain. By single antibody variable domain or immunoglobulin single variable domain is meant a folded polypeptide domain comprising sequences characteristic of antibody variable domains. It therefore includes complete antibody variable domains and modified variable domains, for example in which one or more loops have been replaced by sequences which are not characteristic of antibody variable domains, or antibody variable domains which have been truncated or comprise N- or C-terminal extensions, as well as folded fragments of variable domains which retain at least in part the binding activity and specificity of the full-length domain.

Immunoglobulin single variable domain: The phrase “immunoglobulin single variable domain” refers to an antibody variable domain (V_(H), V_(HH), V_(L)) or binding domain that specifically binds an antigen or epitope independently of different or other V regions or domains i.e. is monovalent. An immunoglobulin single variable domain can be present in a format (e.g., homo- or hetero-multimer) with other variable regions or variable domains where the other regions or domains are not required for antigen binding by the single immunoglobulin variable domain (i.e., where the immunoglobulin single variable domain binds antigen independently of the additional variable domains). A “domain antibody” or “dAb” is an “immunoglobulin single variable domain” as the term is used herein. A “single antibody variable domain” or an “antibody single variable domain” is the same as an “immunoglobulin single variable domain” as the term is used herein. An immunoglobulin single variable domain is in one embodiment a human antibody variable domain, but also includes single antibody variable domains from other species such as rodent (for example, as disclosed in WO 00/29004, the contents of which are incorporated herein by reference in their entirety), nurse shark and Camelid VHH dAbs. Camelid VHH are immunoglobulin single variable domain polypeptides that are derived from species including camel, llama, alpaca, dromedary, and guanaco, which produce heavy chain antibodies naturally devoid of light chains. The VHH may be humanized.

In all aspects of the disclosure, the immunoglobulin single variable domain is independently selected from antibody heavy chain and light chain single variable domains, e.g. V_(H), V_(L) and V_(HH).

As used herein an “antibody” refers to IgG, IgM, IgA, IgD or IgE or a fragment (such as a Fab, F(ab′)₂, Fv, disulphide linked Fv, scFv, closed conformation multispecific antibody, disulphide-linked scFv, diabody) whether derived from any species naturally producing an antibody, or created by recombinant DNA technology; whether isolated from, for example, serum, B-cells, hybridomas, transfectomas, yeast or bacteria.

Antibody format: In one embodiment, the immunoglobulin single variable domain, polypeptide or ligand in accordance with the disclosure can be provided in any antibody format. As used herein, “antibody format” refers to any suitable polypeptide structure in which one or more antibody variable domains can be incorporated so as to confer binding specificity for antigen on the structure. A variety of suitable antibody formats are known in the art, such as, chimeric antibodies, humanized antibodies, human antibodies, single chain antibodies, bispecific antibodies, antibody heavy chains, antibody light chains, homodimers and heterodimers of antibody heavy chains and/or light chains, antigen-binding fragments of any of the foregoing (e.g., a Fv fragment (e.g., single chain Fv (scFv), a disulfide bonded Fv), a Fab fragment, a Fab′ fragment, a F(ab′)₂ fragment), a single antibody variable domain (e.g., a dAb, V_(H), V_(HH), VD, and modified versions of any of the foregoing (e.g., modified by the covalent attachment of polyethylene glycol or other suitable polymer or a humanized V_(HH)). In one embodiment, alternative antibody formats include alternative scaffolds in which the CDRs of any molecules in accordance with the disclosure can be grafted onto a suitable protein scaffold or skeleton, such as an affibody, a SpA scaffold, an LDL receptor class A domain, an avimer (see, e.g., U.S. Patent Application Publication Nos. 2005/0053973, 2005/0089932, 2005/0164301) or an EGF domain. Further, the ligand can be bivalent (heterobivalent) or multivalent (heteromultivalent) as described herein. In other embodiments, a “Universal framework” may be used wherein “Universal framework” refers to a single antibody framework sequence corresponding to the regions of an antibody conserved in sequence as defined by Kabat (“Sequences of Proteins of Immunological Interest”, US Department of Health and Human Services) or corresponding to the human germline immunoglobulin repertoire or structure as defined by Chothia and Lesk, (1987) J. Mol. Biol. 196:910-917. The disclosure provides for the use of a single framework, or a set of such frameworks, which has been found to permit the derivation of virtually any binding specificity through variation in the hypervariable regions alone.

In embodiments of the disclosure described throughout this disclosure, instead of the use of an anti-TGFbetaRII “dAb” in a peptide or ligand of the disclosure, it is contemplated that one of ordinary skill in the art can use a polypeptide or domain that comprises one or more or all 3 of the CDRs of a dAb of the disclosure that binds TGFbetaRII (e.g., CDRs grafted onto a suitable protein scaffold or skeleton, e.g. an affibody, an SpA scaffold, an LDL receptor class A domain or an EGF domain). The disclosure as a whole is to be construed accordingly to provide disclosure of polypeptides using such domains in place of a dAb. In this respect, see WO2008096158, the disclosure of which is incorporated by reference.

In one embodiment, the anti-TGFbetaRII immunoglobulin single variable domain is any suitable immunoglobulin variable domain, and optionally is a human variable domain or a variable domain that comprises or is derived from a human framework region (e.g., DP47 or DPK9 framework regions).

Antigen: As described herein an “antigen” is a molecule that is bound by a binding domain according to the present disclosure. Typically, antigens are bound by antibody ligands and are capable of raising an antibody response in vivo. It may be, for example, a polypeptide, protein, nucleic acid or other molecule.

Epitope: An “epitope” is a unit of structure conventionally bound by an immunoglobulin V_(H)/V_(L) pair. Epitopes define the minimum binding site for an antibody, and thus represent the target of specificity of an antibody. In the case of a single domain antibody, an epitope represents the unit of structure bound by a variable domain in isolation.

Binding: Typically, specific binding is indicated by a dissociation constant (Kd) of 50 nanomolar or less, optionally 250 picomolar or less. Specific binding of an antigen-binding protein to an antigen or epitope can be determined by a suitable assay, including, for example, Scatchard analysis and/or competitive binding assays, such as radioimmunoassays (RIA), enzyme immunoassays such as ELISA and sandwich competition assays, and the different variants thereof.

Binding affinity: Binding affinity is optionally determined using surface plasmon resonance (SPR) and BIACORE™ (Karlsson et al., 1991), using a BIACORE™ system (Uppsala, Sweden). The BIACORE™ system uses surface plasmon resonance (SPR, Welford K. 1991, Opt. Quant. Elect. 23:1; Morton and Myszka, 1998, Methods in Enzymology 295: 268) to monitor biomolecular interactions in real time, and uses surface plasmon resonance which can detect changes in the resonance angle of light at the surface of a thin gold film on a glass support as a result of changes in the refractive index of the surface up to 300 nm away. BIACORE™ analysis conveniently generates association rate constants, dissociation rate constants, equilibrium dissociation constants, and affinity constants. Binding affinity is obtained by assessing the association and dissociation rate constants using a BIACORE™ surface plasmon resonance system (BIACORE™, Inc.). A biosensor chip is activated for covalent coupling of the target according to the manufacturer's (BIACORE™) instructions. The target is then diluted and injected over the chip to obtain a signal in response units of immobilized material. Since the signal in resonance units (RU) is proportional to the mass of immobilized material, this represents a range of immobilized target densities on the matrix. Dissociation data are fit to a one-site model to obtain k_(off)+/−s.d. (standard deviation of measurements). Pseudo-first order rate constant (Kd's) are calculated for each association curve, and plotted as a function of protein concentration to obtain k_(on)+/−s.e. (standard error of fit). Equilibrium dissociation constants for binding, Kd's, are calculated from SPR measurements as k_(off)/k_(on).

Another aspect of the disclosure provides an anti-TGFbetaRII immunoglobulin single variable domain that specifically binds to human TGFbetaRII. In one embodiment, the variable domain binds human TGFbetaRII with an equilibrium dissociation constant (KD) of about 50 nM, 40 nM, 30 nM, 20 nM, 10 nM or less, optionally about 9, 8, 7, 6 or 5 nM or less, optionally about 4 nM or less, about 3 nM or less or about 2 nM or less or about 1 nM or less, optionally about 500 pM or less. Suitably, where the variable domain has an equilibrium dissociation constant in the range of about 50 nM to 500 pM, it is particularly suitable for local administration to a tissue of interest such as the lung. In this embodiment, a high concentration of such a “moderate affinity” binder can be provided as an effective therapeutic. In another embodiment, the variable domain binds human TGFbetaRII with an equilibrium dissociation constant (KD) of about 500 pM or less, optionally about 450 pM, 400 pM, 350 pM, 300 pM, 250 pM, 200 pM, 150 pM, 100 pM, 50 pM or less, optionally about 40 pM, 30 pM, 20 pM, 10 pM or less. Suitably, where the variable domain has a dissociation constant in the range of about 500 pM to 10 pM, it is particularly suitable for systemic administration such that the amount in any one tissue of interest is sufficient to provide an effective therapy. In this embodiment, a low concentration of such a “high affinity” binder can be provided as an effective therapeutic.

In one embodiment, single variable domains of the present disclosure show cross-reactivity between human TGFbetaRII and TGFbetaRII from another species, such as mouse TGFbetaRII. In this embodiment, the variable domains specifically bind human and mouse TGFbetaRII. This is particularly useful, since drug development typically requires testing of lead drug candidates in mouse systems before the drug is tested in humans. The provision of a drug that can bind human and mouse species allows one to test results in these system and make side-by-side comparisons of data using the same drug. This avoids the complication of needing to find a drug that works against a mouse TGFbetaRII and a separate drug that works against human TGFbetaRII, and also avoids the need to compare results in humans and mice using non-identical drugs. Cross reactivity between other species used in disease models such as dog or monkey such as cynomolgus monkey is also envisaged.

Optionally, the binding affinity of the immunoglobulin single variable domain for at least mouse TGFbetaRII and the binding affinity for human TGFbetaRII differ by no more than a factor of 10, 50 or 100.

CDRs: The immunoglobulin single variable domains (dAbs) described herein contain complementarity determining regions (CDR1, CDR2 and CDR3). The locations of CDRs and frame work (FR) regions and a numbering system have been defined by Kabat et al. (Kabat, E. A. et al., Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, U.S. Government Printing Office (1991)). The amino acid sequences of the CDRs (CDR1, CDR2, CDR3) of the V_(H) (CDRH1 etc.) and V_(L) (CDRL1 etc.) (Vκ) dAbs disclosed herein will be readily apparent to the person of skill in the art based on the well known Kabat amino acid numbering system and definition of the CDRs. According to the Kabat numbering system, the most commonly used method based on sequence variability, heavy chain CDR-H3 have varying lengths, insertions are numbered between residue H100 and H101 with letters up to K (i.e. H100, H100A . . . H100K, H101). CDRs can alternatively be determined using the system of Chothia (based on location of the structural loop regions) (Chothia et al., (1989) Conformations of immunoglobulin hypervariable regions; Nature 342, p877-883), according to AbM (compromise between Kabat and Chothia) or according to the Contact method (based on crystal structures and prediction of contact residues with antigen) as follows. See http://www.bioinf.org.uk/abs/for suitable methods for determining CDRs.

Once each residue has been numbered, one can then apply the following CDR definitions:

Kabat:

CDR H1: 31-35/35A/35B

CDR H2: 50-65

CDR H3: 95-102

CDR L1: 24-34

CDR L2: 50-56

CDR L3: 89-97

Chothia:

CDR H1: 26-32

CDR H2: 52-56

CDR H3: 95-102

CDR L1: 24-34

CDR L2: 50-56

CDR L3: 89-97

AbM:

(using Kabat numbering): (using Chothia numbering):

CDR H1: 26-35/35A/35B 26-35

CDR H2: 50-58 -

CDR H3: 95-102 -

CDR L1: 24-34 -

CDR L2: 50-56 -

CDR L3: 89-97 -

Contact:

(using Kabat numbering): (using Chothia numbering):

CDR H1: 30-35/35A/35B 30-35 -

CDR H2: 47-58 -

CDR H3: 93-101 -

CDR L1: 30-36 -

CDR L2: 46-55 -

CDR L3: 89-96 -

(“-” means the same numbering as Kabat)

Accordingly, a person skilled in the art is able to deduce from a given single variable domain sequence, e.g. one having a sequence as set out in any one of SEQ ID NO:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285 and 287 which CDR sequences are contained within them using the various methods outlined herein. For example, for a given single variable domain sequence e.g. SEQ ID NO:1 a skilled person is able to determine the CDR1, CDR2 and CDR3 sequences contained therein using any one or a combination of the CDR definition methods mentioned above. When using the Kabat CDR definition, the skilled person is able to determine that CDR1, CDR2 and CDR 3 sequences are those set forth in SEQ ID NO:77, 113 and 149 respectively. Suitably, CDR sequences are determined using the method of Kabat described herein. In one embodiment, the CDR sequences of each sequence are those set out in tables 1, 2, 9 and 13. In an embodiment a CDR1 sequence is a CDR1 sequence selected from SEQ ID NO:77-112, 241, 244, 247, and 250. In an embodiment a CDR2 sequence is a CDR2 sequence selected from SEQ ID NO:113-148, 242, 245, 248, and 251. In an embodiment a CDR3 sequence is a CDR3 sequence selected from SEQ ID NO:149-184, 243, 246, 249, and 252.

A CDR variant or variant binding unit includes an amino acid sequence modified by at least one amino acid, wherein said modification can be chemical or a partial alteration of the amino acid sequence (for example by no more than 10 amino acids), which modification permits the variant to retain the biological characteristics of the unmodified sequence. For example, the variant is a functional variant which specifically binds to TGFbetaRII. A partial alteration of the CDR amino acid sequence may be by deletion or substitution of one to several amino acids, or by addition or insertion of one to several amino acids, or by a combination thereof (for example by no more than 10 amino acids). The CDR variant or binding unit variant may contain 1, 2, 3, 4, 5 or 6 amino acid substitutions, additions or deletions, in any combination, in the amino acid sequence. The CDR variant or binding unit variant may contain 1, 2 or 3 amino acid substitutions, insertions or deletions, in any combination, in the amino acid sequence. The substitutions in amino acid residues may be conservative substitutions, for example, substituting one hydrophobic amino acid for an alternative hydrophobic amino acid. For example leucine may be substituted with valine, or isoleucine.

TGFbetaRII: As used herein “TGFbetaRII” (transforming growth factor beta type II receptor; TGFβRII) refers to naturally occurring or endogenous mammalian TGFbetaRII proteins and to proteins having an amino acid sequence which is the same as that of a naturally occurring or endogenous corresponding mammalian TGFbetaRII protein (e.g., recombinant proteins, synthetic proteins (i.e., produced using the methods of synthetic organic chemistry)). Accordingly, as defined herein, the term includes mature TGFbetaRII protein, polymorphic or allelic variants, and other isoforms of TGFbetaRII and modified or unmodified forms of the foregoing (e.g., lipidated, glycosylated). Naturally occurring or endogenous TGFbetaRII includes wild type proteins such as mature TGFbetaRII, polymorphic or allelic variants and other isoforms and mutant forms which occur naturally in mammals (e.g., humans, non-human primates). Such proteins can be recovered or isolated from a source which naturally expresses TGFbetaRII, for example. These proteins and proteins having the same amino acid sequence as a naturally occurring or endogenous corresponding TGFbetaRII, are referred to by the name of the corresponding mammal. For example, where the corresponding mammal is a human, the protein is designated as a human TGFbetaRII. Human TGFbetaRII is described, for example, by Lin, et al., Cell 1992, Vol. 68(4), p. 775-785 and GenBank Accession No. M85079.

Human TGFbetaRII is a transmembrane receptor consisting of 567 amino acids with an extracellular domain of approximately 159 amino acids, a transmembrane domain and a cytoplasmic domain which comprises a protein kinase domain for signal transduction.

As used herein “TGFbetaRII” also includes a portion or fragment of TGFbetaRII. In one embodiment, such a portion or fragment includes the extracellular domain of TGFbetaRII or a portion thereof.

By “anti-TGFbetaRII” with reference to an immunoglobulin single variable domain, polypeptide, ligand, fusion protein or so forth is meant a moiety which recognises and binds TGFbetaRII. In one embodiment an “anti-TGFbetaRII” specifically recognises and/or specifically binds to the protein TGFbetaRII, and, suitably, human TGFbetaRII. In another embodiment, the anti-TGFbetaRII immunoglobulin single variable domain in accordance with the disclosure also binds to mouse TGFbetaRII (GenBank accession number NM_(—)029575; described, for example in Massague et al., Cell 69 (7), 1067-1070 (1992)).

“TGFbeta” includes isoforms such as TGFbeta1, TGFbeta2 and TGFbeta3.

TGFbeta binds TGFbetaRII and, in a complex with TGFbetaRI initiates a signaling pathway. Accordingly, TGFbeta activity and inhibition or neutralization of TGFbeta activity can be determined through any assay which measures an output of TGFbeta signaling. TGFbeta signaling is reviewed, for example in Itoh, et al., Eur. J. Biochem 2000, Vol. 267, p. 6954; Dennler, et al., Journal of Leucocyte Biol. 2002, 71(5), p. 731-40. Thus, TGFbeta activity can be tested in a number of different assays familiar to the person skilled in the art. “Inhibition” or “Neutralization” means that a biological activity of TGFbeta is reduced either totally or partially in the presence of the immunoglobulin single variable domain of the present disclosure in comparison to the activity of TGFbeta in the absence of such immunoglobulin single variable domain.

In one embodiment, an inhibition or neutralisation of TGFbeta activity is tested in an IL-11 release assay. In this embodiment, the ability of the immunoglobulin single variable domain in accordance with the disclosure is tested for its ability to inhibit human TGFbeta1 (TGFbeta1; TGF-β1) stimulated IL-11 release from cells such as A549 cells. TGFbeta1 (TGF-β1) binds directly to TGFbetaRII (TGF-βRII) and induces the assembly of the TGFbetaRI/RII (TGF-βRI/II) complex. TGFbetaRI (TGF-βRI) is phosphorylated and is able to signal through several pathways including the Smad 4 pathway. Activation of the Smad 4 pathway results in the release of IL-11. The IL-11 is secreted into the cell supernatant and is then measured by colourmetric ELISA. Suitable IL-11 release assays are described herein, such as the Human IL-11 Quantikine ELISA assay kit supplied by R & D systems (ref. D1100).

In another embodiment, TGFbeta activity is tested in an assay for the ability of the immunoglobulin single variable domain in accordance with the disclosure to inhibit TGFbeta-induced expression of CAGA-luciferase in MC3T3-E1 cells in a MC3T3-E1 luciferase assay. Three copies of a TGFbeta-responsive sequence motif, termed a CAGA box are present in the human PAI-1 promoter and specifically binds Smad3 and 4 proteins. Cloning multiple copies of the CAGA box into a luciferase reporter construct confers TGFbeta responsiveness to cells transfected with the reporter system. One suitable assay is described herein and uses MC3T3-E1 cells (mouse osteoblasts) stably transfected with a [CAGA]₁₂-luciferase reporter construct (Dennler, et al., (1998) EMBO J. 17, 3091-3100).

Other suitable assays include a human SBE beta-lactamase cell assay (INVITROGEN®, cell sensor assay). Examples of suitable assays are described herein.

Suitably, the immunoglobulin single variable domain, polypeptide, ligand or fusion protein in accordance with the disclosure does not, itself activate TGFbetaRII receptor signalling. Accordingly, in one embodiment, the immunoglobulin single variable domain, polypeptide, ligand or fusion protein in accordance with the disclosure is devoid of agonist activity at 10 μM. Agonist activity can be determined by testing a compound of interest in a TGFbetaRII assay as described herein in the absence of TGFbeta. Where TGFbeta is absent, agonist activity of a compound of interest would be detected by detecting TGFbetaRII signalling.

Homology: Sequences similar or homologous (e.g., at least about 70% sequence identity) to the sequences disclosed herein are also part of the disclosure. In some embodiments, the sequence identity at the amino acid level can be about 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or higher. At the nucleic acid level, the sequence identity can be about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or higher. Alternatively, substantial identity exists when the nucleic acid segments will hybridize under selective hybridization conditions (e.g., very high stringency hybridization conditions), to the complement of the strand. The nucleic acids may be present in whole cells, in a cell lysate, or in a partially purified or substantially pure form.

As used herein, the terms “low stringency,” “medium stringency,” “high stringency,” or “very high stringency” conditions describe conditions for nucleic acid hybridization and washing. Guidance for performing hybridization reactions can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6, which is incorporated herein by reference in its entirety. Aqueous and nonaqueous methods are described in that reference and either can be used. Specific hybridization conditions referred to herein are as follows: (1) low stringency hybridization conditions in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by two washes in 0.2×SSC, 0.1% SDS at least at 50° C. (the temperature of the washes can be increased to 55° C. for low stringency conditions); (2) medium stringency hybridization conditions in 6×SSC at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 60° C.; (3) high stringency hybridization conditions in 6×SSC at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 65° C.; and optionally (4) very high stringency hybridization conditions are 0.5M sodium phosphate, 7% SDS at 65° C., followed by one or more washes at 0.2×SSC, 1% SDS at 65° C. Very high stringency conditions (4) are the preferred conditions and the ones that should be used unless otherwise specified.

Calculations of “homology” or “sequence identity” or “similarity” between two sequences (the terms are used interchangeably herein) are performed as follows. The sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). In one embodiment, the length of a reference sequence aligned for comparison purposes is at least about 30%, optionally at least about 40%, optionally at least about 50%, optionally at least about 60%, and optionally at least about 70%, 80%, 85% 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% of the length of the reference sequence. The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position (as used herein amino acid or nucleic acid “homology” is equivalent to amino acid or nucleic acid “identity”). The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences.

Amino acid and nucleotide sequence alignments and homology, similarity or identity, as defined herein are optionally prepared and determined using the algorithm BLAST 2 Sequences, using default parameters (Tatusova, T. A. et al., FEMS Microbiol Lett, 174:187-188 (1999)). Alternatively, the BLAST algorithm (version 2.0) is employed for sequence alignment, with parameters set to default values. BLAST (Basic Local Alignment Search Tool) is the heuristic search algorithm employed by the programs blastp, blastn, blastx, tblastn, and tblastx; these programs ascribe significance to their findings using the statistical methods of Karlin and Altschul, 1990, Proc. Natl. Acad. Sci. USA 87(6):2264-8.

Ligand: As used herein, the term “ligand” refers to a compound that comprises at least one peptide, polypeptide or protein moiety that has a binding site with binding specificity for TGFbetaRII. A ligand can also be referred to as a “binding moiety”.

The ligands or binding moieties according to the disclosure optionally comprise immunoglobulin variable domains which have different binding specificities, and do not contain variable domain pairs which together form a binding site for target compound (i.e., do not comprise an immunoglobulin heavy chain variable domain and an immunoglobulin light chain variable domain that together form a binding site for TGFbetaRII). Optionally, each domain which has a binding site that has binding specificity for a target is an immunoglobulin single variable domain (e.g., immunoglobulin single heavy chain variable domain (e.g., V_(H), V_(HH)), immunoglobulin single light chain variable domain (e.g., V_(I))) that has binding specificity for a desired target (e.g., TGFbetaRII).

Thus, “ligands” include polypeptides that comprise two or more immunoglobulin single variable domains wherein each immunoglobulin single variable domain binds to a different target. Ligands also include polypeptides that comprise at least two immunoglobulin single variable domains or the CDR sequences of the single variable domains that bind different targets in a suitable format, such as an antibody format (e.g., IgG-like format, scFv, Fab, Fab′, F(ab′)₂) or a suitable protein scaffold or skeleton, such as an affibody, a SpA scaffold, an LDL receptor class A domain, an EGF domain, avimer and dual- and multi-specific ligands as described herein.

The polypeptide domain which has a binding site that has binding specificity for a target (e.g., TGFbetaRII) can also be a protein domain comprising a binding site for a desired target, e.g., a protein domain is selected from an affibody, a SpA domain, an LDL receptor class A domain, an avimer (see, e.g., U.S. Patent Application Publication Nos. 2005/0053973, 2005/0089932, 2005/0164301). If desired, a “ligand” can further comprise one or more additional moieties that can each independently be a peptide, polypeptide or protein moiety or a non-peptidic moiety (e.g., a polyalkylene glycol, a lipid, a carbohydrate). For example, the ligand can further comprise a half-life extending moiety as described herein (e.g., a polyalkylene glycol moiety, a moiety comprising albumin, an albumin fragment or albumin variant, a moiety comprising transferrin, a transferrin fragment or transferrin variant, a moiety that binds albumin, a moiety that binds neonatal Fc receptor).

Competes: As referred to herein, the term “competes” means that the binding of a first target (e.g., TGFbetaRII) to its cognate target binding domain (e.g., immunoglobulin single variable domain) is inhibited in the presence of a second binding domain (e.g., immunoglobulin single variable domain) that is specific for said cognate target. For example, binding may be inhibited sterically, for example by physical blocking of a binding domain or by alteration of the structure or environment of a binding domain such that its affinity or avidity for a target is reduced. See WO2006038027 for details of how to perform competition ELISA and competition BIACORE™ experiments to determine competition between first and second binding domains, the details of which are incorporated herein by reference to provide explicit disclosure for use in the present disclosure. The disclosure includes antigen binding proteins, specifically single variable domains, polypeptides, ligands and fusion proteins, that compete with any one of single variable domains of SEQ ID NO:1-38. In a particular embodiment there is provided a TGFbetaRII binding protein which competes with any one of single variable domains of SEQ ID NO:1-38 and also has a KD of 50 nM or less to TGFbetaRII. In a particular embodiment the KD is between 10 pM and 50 nM. In a particular embodiment, the KD is between 10 pM and 10 nM. In a particular embodiment, the KD is between 100 pM and 10 nM. In a particular embodiment the KD is approximately 100 pM.

TGFbeta signaling: Suitably, the single variable domain, polypeptide or ligand of the disclosure can neutralize TGFbeta signaling through TGFbetaRII. By “neutralizing”, it is meant that the normal signaling effect of TGFbeta is blocked such that the presence of TGFbeta has a neutral effect on TGFbetaRII signaling. Suitable methods for measuring a neutralizing effect include assays for TGFbeta signaling as described herein. In one embodiment, neutralization is observed as a % inhibition of TFGbeta activity in a TGFbeta signaling assay. In one embodiment, the single variable domain or polypeptide binds to the extracellular domain of TGFbetaRII thereby inhibiting/blocking the binding of TGFbeta to the extracellular domain of TGFbetaRII. Suitably, the single variable domain or polypeptide is useful where there is an excess of bioavailable TGFbeta and the single variable domain or polypeptide serves to inhibit the signaling activity of the bioavailable TGFbeta through inhibiting binding or TGFbeta to its cognate receptor TGFbetaRII.

As used herein, the term “antagonist of TGFbetaRII” or “anti-TGFbetaRII antagonist” or the like refers to an agent (e.g., a molecule, a compound) which binds TGFbetaRII and can inhibit a (i.e., one or more) function of TGFbetaRII. For example, an antagonist of TGFbetaRII can inhibit the binding of TGFbeta to TGFbetaRII and/or inhibit signal transduction mediated through TGFbetaRII. Accordingly, TGFbeta-mediated processes and cellular responses can be inhibited with an antagonist of TGFbetaRII.

In one embodiment, the ligand (e.g., immunoglobulin single variable domain) that binds TGFbetaRII inhibits binding of TGFbeta to a TGFbetaRII receptor with an inhibitory concentration 50 (IC50) that is ≦about 10 μM, ≦about 1 μM, ≦about 100 nM, ≦about 50 nM, ≦about 10 nM, ≦about 5 nM, ≦about 1 nM, ≦about 500 pM, ≦about 300 pM, ≦about 100 pM, or about 10 pM. In a particular embodiment, an anti-TGFbetaRII immunoglobulin single variable domain of the disclosure has an IC50 of 15 μM or less. The IC50 is optionally determined using an in vitro TGFbeta receptor binding assay, or cell assay, such as the assay described herein.

It is also contemplated that the ligand (e.g., immunoglobulin single variable domain) optionally inhibit TGFbetaRII induced functions in a suitable in vitro assay with a neutralizing dose 50 (ND50) that is ≦about 10 μM, ≦about 1 μM, ≦about 100 nM, ≦about 50 nM, ≦about 10 nM, about 5 nM, ≦about 1 nM, ≦about 500 pM, ≦about 300 pM, ≦about 100 pM, ≦about 10 pM, ≦about 1 pM about 500 fM, ≦about 300 fM, ≦about 100 fM, ≦about 10 fM. In a particular embodiment, an anti-TGFbetaRII immunoglobulin single variable domain of the disclosure achieves greater than 40% neutralisation of TGF-β.

“dual-specific ligand”: In one embodiment, the immunoglobulin single variable domain, polypeptide or ligand in accordance with the disclosure can be part of a “dual-specific ligand” which refers to a ligand comprising a first antigen or epitope binding site (e.g., first immunoglobulin single variable domain) and a second antigen or epitope binding site (e.g., second immunoglobulin single variable domain), wherein the binding sites or variable domains are capable of binding to two antigens (e.g., different antigens or two copies of the same antigen) or two epitopes on the same antigen which are not normally bound by a monospecific immunoglobulin. For example, the two epitopes may be on the same antigen, but are not the same epitope or sufficiently adjacent to be bound by a monospecific ligand. In one embodiment, dual-specific ligands according to the disclosure are composed of binding sites or variable domains which have different specificities, and do not contain mutually complementary variable domain pairs (i.e. V_(H)/V_(L) pairs) which have the same specificity (i.e., do not form a unitary binding site).

In one embodiment, a “dual-specific ligand” may bind to TGFbetaRII and to another target molecule. For example, another target molecule may be a tissue-specific target molecule such that the dual-specific ligand of the disclosure enables an anti-TGFbetaRII polypeptide or immunoglobulin single variable domain in accordance with the disclosure to be targeted to a tissue of interest. Such tissues include lung, liver and so forth.

Multispecific dAb multimers are also provided. This includes a dAb multimer comprising an anti-TGFbetaRII immunoglobulin single variable domain according to any aspect of the disclosure and one or more single variable domains each of which binds to a different target (e.g. a target other than TGFbetaRII). In an embodiment a bispecific dAb multimer is provided e.g. a dab multimer comprising one or more anti-TGFbetaRII immunoglobulin single variable domains according to any aspect of the disclosure and one or more dabs which bind to a second, different target. In an embodiment a trispecific dAb multimer is provided.

The ligands of the disclosure (e.g., polypeptides, dAbs and antagonists) can be formatted as a fusion protein that contains a first immunoglobulin single variable domain that is fused directly to a second immunoglobulin single variable domain. If desired such a format can further comprise a half-life extending moiety. For example, the ligand can comprise a first immunoglobulin single variable domain that is fused directly to a second immunoglobulin single variable domain that is fused directly to an immunoglobulin single variable domain that binds serum albumin.

Generally, the orientation of the polypeptide domains that have a binding site with binding specificity for a target, and whether the ligand comprises a linker, is a matter of design choice. However, some orientations, with or without linkers, may provide better binding characteristics than other orientations. All orientations (e.g., dAb1-linker-dAb2; dAb2-linker-dAb1) are encompassed by the disclosure are ligands that contain an orientation that provides desired binding characteristics can be easily identified by screening.

Polypeptides and dAbs according to the disclosure, including dAb monomers, dimers and trimers, can be linked to an antibody Fc region, comprising one or both of C_(H)2 and C_(H)3 domains, and optionally a hinge region. For example, vectors encoding ligands linked as a single nucleotide sequence to an Fc region may be used to prepare such polypeptides. In an embodiment there is provided a dAb-Fc fusion.

The disclosure moreover provides dimers, trimers and polymers of the aforementioned dAb monomers.

Target: As used herein, the phrase “target” refers to a biological molecule (e.g., peptide, polypeptide, protein, lipid, carbohydrate) to which a polypeptide domain which has a binding site can bind. The target can be, for example, an intracellular target (e.g., an intracellular protein target), a soluble target (e.g., a secreted), or a cell surface target (e.g., a membrane protein, a receptor protein). In one embodiment, the target is TGFbetaRII. In another embodiment, the target is TGFbetaRII extracellular domain.

Complementary: As used herein “complementary” refers to when two immunoglobulin domains belong to families of structures which form cognate pairs or groups or are derived from such families and retain this feature. For example, a V_(H) domain and a V_(L) domain of an antibody are complementary; two V_(H) domains are not complementary, and two V_(L) domains are not complementary. Complementary domains may be found in other members of the immunoglobulin superfamily, such as the Vα and Vβ (or γ and δ) domains of the T-cell receptor. Domains which are artificial, such as domains based on protein scaffolds which do not bind epitopes unless engineered to do so, are non-complementary. Likewise, two domains based on (for example) an immunoglobulin domain and a fibronectin domain are not complementary.

“Affinity” and “avidity” are terms of art that describe the strength of a binding interaction. With respect to the ligands of the disclosure, avidity refers to the overall strength of binding between the targets (e.g., first target and second target) on the cell and the ligand. Avidity is more than the sum of the individual affinities for the individual targets.

Nucleic acid molecules, vectors and host cells: The disclosure also provides isolated and/or recombinant nucleic acid molecules encoding ligands (single variable domains, fusion proteins, polypeptides, dual-specific ligands and multispecific ligands) as described herein.

Nucleic acids referred to herein as “isolated” are nucleic acids which have been separated away from the nucleic acids of the genomic DNA or cellular RNA of their source of origin (e.g., as it exists in cells or in a mixture of nucleic acids such as a library), and include nucleic acids obtained by methods described herein or other suitable methods, including essentially pure nucleic acids, nucleic acids produced by chemical synthesis, by combinations of biological and chemical methods, and recombinant nucleic acids which are isolated (see e.g., Daugherty, B. L. et al., Nucleic Acids Res., 19(9): 2471 2476 (1991); Lewis, A. P. and J. S. Crowe, Gene, 101: 297-302 (1991)).

Nucleic acids referred to herein as “recombinant” are nucleic acids which have been produced by recombinant DNA methodology, including those nucleic acids that are generated by procedures which rely upon a method of artificial recombination, such as the polymerase chain reaction (PCR) and/or cloning into a vector using restriction enzymes.

In certain embodiments, the isolated and/or recombinant nucleic acid comprises a nucleotide sequence encoding an immunoglobulin single variable domain, polypeptide or ligand, as described herein, wherein said ligand comprises an amino acid sequence that has at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% amino acid sequence identity with the amino acid sequence of a dAb that binds TGFbetaRII disclosed herein, e.g. amino acid sequences set out in any of SEQ ID NOS: 1-38. Nucleotide sequence identity can be determined over the whole length of the nucleotide sequence that encodes the selected anti-TGFbetaRII dAb. In an embodiment the nucleic acid sequence comprises or consists of a nucleic acid sequence at least 80% identical to of any one of SEQ ID NO:39-66. In an embodiment the nucleic acid sequence comprises or consists of a nucleic acid sequence of any one of SEQ ID NO:39-76.

Embodiments of the disclosure also provide codon optimized nucleotide sequences encoding polypeptides and variable domains as disclosed herein e.g. optimised for expression in bacterial, mammalian or yeast cells.

The disclosure also provides a vector comprising a recombinant nucleic acid molecule of the disclosure. In certain embodiments, the vector is an expression vector comprising one or more expression control elements or sequences that are operably linked to the recombinant nucleic acid of the disclosure. The disclosure also provides a recombinant host cell comprising a recombinant nucleic acid molecule or vector of the disclosure. Suitable vectors (e.g., plasmids, phagemids), expression control elements, host cells and methods for producing recombinant host cells of the disclosure are well-known in the art, and examples are further described herein.

Suitable expression vectors can contain a number of components, for example, an origin of replication, a selectable marker gene, one or more expression control elements, such as a transcription control element (e.g., promoter, enhancer, terminator) and/or one or more translation signals, a signal sequence or leader sequence, and the like. Expression control elements and a signal sequence, if present, can be provided by the vector or other source. For example, the transcriptional and/or translational control sequences of a cloned nucleic acid encoding an antibody chain can be used to direct expression.

A promoter can be provided for expression in a desired host cell. Promoters can be constitutive or inducible. For example, a promoter can be operably linked to a nucleic acid encoding an antibody, antibody chain or portion thereof, such that it directs transcription of the nucleic acid. A variety of suitable promoters for prokaryotic (e.g., lac, tac, T3, T7 promoters for E. coli) and eukaryotic (e.g., Simian Virus 40 early or late promoter, Rous sarcoma virus long terminal repeat promoter, cytomegalovirus promoter, adenovirus late promoter) hosts are available.

In addition, expression vectors typically comprise a selectable marker for selection of host cells carrying the vector, and, in the case of a replicable expression vector, an origin of replication. Genes encoding products which confer antibiotic or drug resistance are common selectable markers and may be used in prokaryotic (e.g., lactamase gene (ampicillin resistance), Tet gene for tetracycline resistance) and eukaryotic cells (e.g., neomycin (G418 or geneticin), gpt (mycophenolic acid), ampicillin, or hygromycin resistance genes). Dihydrofolate reductase marker genes permit selection with methotrexate in a variety of hosts. Genes encoding the gene product of auxotrophic markers of the host (e.g., LEU2, URA3, HIS3) are often used as selectable markers in yeast. Use of viral (e.g., baculovirus) or phage vectors, and vectors which are capable of integrating into the genome of the host cell, such as retroviral vectors, are also contemplated. Suitable expression vectors for expression in mammalian cells and prokaryotic cells (E. coli), insect cells (Drosophila Schnieder S2 cells, Sf9) and yeast (P. methanolica, P. pastoris, S. cerevisiae) are well-known in the art.

Suitable host cells can be prokaryotic, including bacterial cells such as E. coli, B. subtilis and/or other suitable bacteria; eukaryotic cells, such as fungal or yeast cells (e.g., Pichia pastoris, Aspergillus sp., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Neurospora crassa), or other lower eukaryotic cells, and cells of higher eukaryotes such as those from insects (e.g., Drosophila Schnieder S2 cells, Sf9 insect cells (WO 94/26087 (O'Connor)), mammals (e.g., COS cells, such as COS-1 (ATCC Accession No. CRL-1650) and COS-7 (ATCC Accession No. CRL-1651), CHO (e.g., ATCC Accession No. CRL-9096, CHO DG44 (Urlaub, G. and Chasin, L A., Proc. Natl. Acad. Sci. USA, 77(7):4216-4220 (1980))), 293 (ATCC Accession No. CRL-1573), HeLa (ATCC Accession No. CCL-2), CV1 (ATCC Accession No. CCL-70), WOP (Dailey, L., et al., J. Virol., 54:739-749 (1985), 3T3, 293T (Pear, W. S., et al., Proc. Natl. Acad. Sci. U.S.A., 90:8392-8396 (1993)) NS0 cells, SP2/0, HuT 78 cells and the like, or plants (e.g., tobacco). (See, for example, Ausubel, F. M. et al., eds. Current Protocols in Molecular Biology, Greene Publishing Associates and John Wiley & Sons Inc. (1993). In some embodiments, the host cell is an isolated host cell and is not part of a multicellular organism (e.g., plant or animal). In certain embodiments, the host cell is a non-human host cell. The disclosure also provides a method for producing a ligand (e.g., dual-specific ligand, multispecific ligand) of the disclosure, comprising maintaining a recombinant host cell comprising a recombinant nucleic acid of the disclosure under conditions suitable for expression of the recombinant nucleic acid, whereby the recombinant nucleic acid is expressed and a ligand is produced. In some embodiments, the method further comprises isolating the ligand.

Reference is made to WO200708515, page 161, line 24 to page 189, line 10 for details of disclosure that is applicable to embodiments of the present disclosure. This disclosure is hereby incorporated herein by reference as though it appears explicitly in the text of the present disclosure and relates to the embodiments of the present disclosure, and to provide explicit support for disclosure to incorporated into claims below. This includes disclosure presented in WO200708515, page 161, line 24 to page 189, line 10 providing details of the “Preparation of Immunoglobulin Based Ligands”, “Library vector systems”, “Library Construction”, “Combining Single Variable Domains”, “Characterisation of Ligands”, “Structure of Ligands”, “Skeletons”, “Protein Scaffolds”, “Scaffolds for Use in Constructing Ligands”, “Diversification of the Canonical Sequence” and “Therapeutic and diagnostic compositions and uses”, as well as definitions of “operably linked”, “naive”, “prevention”, “suppression”, “treatment”, “allergic disease”, “Th2-mediated disease”, “therapeutically-effective dose” and “effective”.

The phrase, “half-life” refers to the time taken for the serum concentration of the immunoglobulin single variable domain, polypeptide or ligand to reduce by 50%, in vivo, for example due to degradation of the ligand and/or clearance or sequestration of the ligand by natural mechanisms. The ligands of the disclosure can be stabilized in vivo and their half-life increased by binding to molecules which resist degradation and/or clearance or sequestration. Typically, such molecules are naturally occurring proteins which themselves have a long half-life in vivo. The half-life of a ligand is increased if its functional activity persists, in vivo, for a longer period than a similar ligand which is not specific for the half-life increasing molecule. Thus a ligand specific for HSA and a target molecules is compared with the same ligand wherein the specificity to HSA is not present, that is does not bind HSA but binds another molecule. Typically, the half-life is increased by 10%, 20%, 30%, 40%, 50% or more. Increases in the range of 2×, 3×, 4×, 5×, 10×, 20×, 30×, 40×, 50× or more of the half-life are possible. Alternatively, or in addition, increases in the range of up to 30×, 40×, 50×, 60×, 70×, 80×, 90×, 100×, 150× of the half life are possible.

Formats: Increased half-life can be useful in in vivo applications of immunoglobulins, especially antibodies and most especially antibody fragments of small size. Such fragments (Fvs, disulphide bonded Fvs, Fabs, scFvs, dAbs) are generally rapidly cleared from the body. dAbs, polypeptides or ligands in accordance with the disclosure can be adapted to provide increased half-life in vivo and consequently longer persistence times in the body of the functional activity of the ligand.

Methods for pharmacokinetic analysis and determination of ligand half-life will be familiar to those skilled in the art. Details may be found in Kenneth, A et al: Chemical Stability of Pharmaceuticals: A Handbook for Pharmacists and in Peters et al, Pharmacokinetic analysis: A Practical Approach (1996). Reference is also made to “Pharmacokinetics”, M Gibaldi & D Perron, published by Marcel Dekker, 2nd Rev. ex edition (1982), which describes pharmacokinetic parameters such as t alpha and t beta half lives and area under the curve (AUC).

Half lives (t½ alpha and t½ beta) and AUC can be determined from a curve of serum concentration of ligand against time. The WINNONLIN™ analysis package (available from Pharsight Corp., Mountain View, Calif. 94040, USA) can be used, for example, to model the curve. In a first phase (the alpha phase) the ligand is undergoing mainly distribution in the patient, with some elimination. A second phase (beta phase) is the terminal phase when the ligand has been distributed and the serum concentration is decreasing as the ligand is cleared from the patient. The t alpha half life is the half life of the first phase and the t beta half life is the half life of the second phase. Thus, in one embodiment, the present disclosure provides a ligand or a composition comprising a ligand according to the disclosure having a tα halflife in the range of 15 minutes or more. In one embodiment, the lower end of the range is 30 minutes, 45 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 10 hours, 11 hours or 12 hours. In addition, or alternatively, a ligand or composition according to the disclosure will have a to half life in the range of up to and including 12 hours. In one embodiment, the upper end of the range is 11, 10, 9, 8, 7, 6 or 5 hours. An example of a suitable range is 1 to 6 hours, 2 to 5 hours or 3 to 4 hours.

In one embodiment, the present disclosure provides a ligand (polypeptide, dAb or antagonist) or a composition comprising a ligand according to the disclosure having a tβ halflife in the range of about 2.5 hours or more. In one embodiment, the lower end of the range is about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 7 hours, about 10 hours, about 11 hours, or about 12 hours. In addition, or alternatively, a ligand or composition according to the disclosure has a tβ halflife in the range of up to and including 21 days. In one embodiment, the upper end of the range is about 12 hours, about 24 hours, about 2 days, about 3 days, about 5 days, about 10 days, about 15 days or about 20 days. In one embodiment a ligand or composition according to the disclosure will have a tβ half life in the range about 12 to about 60 hours. In a further embodiment, it will be in the range about 12 to about 48 hours. In a further embodiment still, it will be in the range about 12 to about 26 hours.

In addition, or alternatively to the above criteria, the present disclosure provides a ligand or a composition comprising a ligand according to the disclosure having an AUC value (area under the curve) in the range of about 1 mg·min/ml or more. In one embodiment, the lower end of the range is about 5, about 10, about 15, about 20, about 30, about 100, about 200 or about 300 mg·min/ml. In addition, or alternatively, a ligand or composition according to the disclosure has an AUC in the range of up to about 600 mg·min/ml. In one embodiment, the upper end of the range is about 500, about 400, about 300, about 200, about 150, about 100, about 75 or about 50 mg·min/ml. In one embodiment a ligand according to the disclosure will have a AUC in the range selected from the group consisting of the following: about 15 to about 150 mg·min/ml, about 15 to about 100 mg·min/ml, about 15 to about 75 mg·min/ml, and about 15 to about 50 mg·min/ml.

Polypeptides and dAbs of the disclosure and antagonists comprising these can be formatted to have a larger hydrodynamic size, for example, by attachment of a PEG group, serum albumin, transferrin, transferrin receptor or at least the transferrin-binding portion thereof, an antibody Fc region, or by conjugation to an antibody domain. For example, polypeptides dAbs and antagonists formatted as a larger antigen-binding fragment of an antibody or as an antibody (e.g., formatted as a Fab, Fab′, F(ab)₂, F(ab′)₂, IgG, scFv).

As used herein, “hydrodynamic size” refers to the apparent size of a molecule (e.g., a protein molecule, ligand) based on the diffusion of the molecule through an aqueous solution. The diffusion or motion of a protein through solution can be processed to derive an apparent size of the protein, where the size is given by the “Stokes radius” or “hydrodynamic radius” of the protein particle. The “hydrodynamic size” of a protein depends on both mass and shape (conformation), such that two proteins having the same molecular mass may have differing hydrodynamic sizes based on the overall conformation of the protein.

Hydrodynamic size of the ligands (e.g., dAb monomers and multimers) of the disclosure may be determined using methods which are well known in the art. For example, gel filtration chromatography may be used to determine the hydrodynamic size of a ligand. Suitable gel filtration matrices for determining the hydrodynamic sizes of ligands, such as cross-linked agarose matrices, are well known and readily available.

The size of a ligand format (e.g., the size of a PEG moiety attached to a dAb monomer), can be varied depending on the desired application. For example, where ligand is intended to leave the circulation and enter into peripheral tissues, it is desirable to keep the hydrodynamic size of the ligand low to facilitate extravazation from the blood stream. Alternatively, where it is desired to have the ligand remain in the systemic circulation for a longer period of time the size of the ligand can be increased, for example by formatting as an Ig like protein.

Half-life extension by targeting an antigen or epitope that increases half-live in vivo: The hydrodynamic size of a ligand and its serum half-life can also be increased by conjugating or associating an TGFbetaRII binding polypeptide, dAb or ligand of the disclosure to a binding domain (e.g., antibody or antibody fragment) that binds an antigen or epitope that increases half-live in vivo, as described herein. For example, the TGFbetaRII binding agent (e.g., polypeptide) can be conjugated or linked to an anti-serum albumin or anti-neonatal Fc receptor antibody or antibody fragment, e.g. an anti-SA or anti-neonatal Fc receptor dAb, Fab, Fab′ or scFv, or to an anti-SA affibody or anti-neonatal Fc receptor Affibody or an anti-SA avimer, or an anti-SA binding domain which comprises a scaffold selected from, but not limited to, the group consisting of CTLA-4, lipocallin, SpA, an affibody, an avimer, GroEI and fibronectin (see WO2008096158 for disclosure of these binding domains, which domains and their sequences are incorporated herein by reference and form part of the disclosure of the present text). Conjugating refers to a composition comprising polypeptide, dAb or antagonist of the disclosure that is bonded (covalently or noncovalently) to a binding domain such as a binding domain that binds serum albumin.

Typically, a polypeptide that enhances serum half-life in vivo is a polypeptide which occurs naturally in vivo and which resists degradation or removal by endogenous mechanisms which remove unwanted material from the organism (e.g., human). For example, a polypeptide that enhances serum half-life in vivo can be selected from proteins from the extracellular matrix, proteins found in blood, proteins found at the blood brain barrier or in neural tissue, proteins localized to the kidney, liver, lung, heart, skin or bone, stress proteins, disease-specific proteins, or proteins involved in Fc transport. Suitable polypeptides are described, for example, in WO2008/096158.

Such an approach can also be used for targeted delivery of a single variable domain, polypeptide or ligand in accordance with the disclosure to a tissue of interest. In one embodiment targeted delivery of a high affinity single variable domain in accordance with the disclosure is provided.

dAbs that Bind Serum Albumin: The disclosure in one embodiment provides a polypeptide or antagonist (e.g., dual specific ligand comprising an anti-TGFbetaRII dAb (a first dAb)) that binds to TGFbetaRII and a second dAb that binds serum albumin (SA), the second dAb binding SA. Details of dual specific ligands are found in WO03002609, WO04003019, WO2008096158 and WO04058821.

In particular embodiments of the ligands and antagonists, the dAb binds human serum albumin and competes for binding to albumin with a dAb selected from the group consisting of any of the dAb sequences disclosed in WO2004003019 (which sequences and their nucleic acid counterpart are incorporated herein by reference and form part of the disclosure of the present text), any of the dAb sequences disclosed in WO2007080392 (which sequences and their nucleic acid counterpart are incorporated herein by reference and form part of the disclosure of the present text), any of the dAb sequences disclosed in WO2008096158 (which sequences and their nucleic acid counterpart are incorporated herein by reference and form part of the disclosure of the present text).

In certain embodiments, the dAb binds human serum albumin and comprises an amino acid sequence that has at least about 80%, or at least about 85%, or at least about 90%, or at least about 95%, or at least about 96%, or at least about 97%, or at least about 98%, or at least about 99% amino acid sequence identity with the amino acid sequence of a dAb described in any of WO2004003019, WO2007080392 or WO2008096158. For example, the dAb that binds human serum albumin can comprise an amino acid sequence that has at least about 90%, or at least about 95%, or at least about 96%, or at least about 97%, or at least about 98%, or at least about 99% amino acid sequence identity with the amino acid sequence of any of these dAbs. In certain embodiments, the dAb binds human serum albumin and comprises an amino acid sequence that has at least about 80%, or at least about 85%, or at least about 90%, or at least about 95%, or at least about 96%, or at least about 97%, or at least about 98%, or at least about 99% amino acid sequence identity with the amino acid sequence of the amino acid sequence of any of these dAbs.

In more particular embodiments, the dAb is a Vκ dAb that binds human serum albumin. In more particular embodiments, the dAb is a V_(H) dAb that binds human serum albumin.

Suitable Camelid V_(HH) that bind serum albumin include those disclosed in WO2004041862 (Ablynx N.V.) and in WO2007080392 (which V_(HH) sequences and their nucleic acid counterpart are incorporated herein by reference and form part of the disclosure of the present text). In certain embodiments, the Camelid V_(HH) binds human serum albumin and comprises an amino acid sequence that has at least about 80%, or at least about 85%, or at least about 90%, or at least about 95%, or at least about 96%, or at least about 97%, or at least about 98%, or at least about 99% amino acid sequence identity with those sequences disclosed in WO2007080392 or any one of SEQ ID NOS:518-534, these sequence numbers corresponding to those cited in WO2007080392 or WO 2004041862.

In an alternative embodiment, the antagonist or ligand comprises a binding moiety specific for TGFbetaRII (e.g., human TGFbetaRII), wherein the moiety comprises non-immunoglobulin sequences as described in WO2008096158, the disclosure of these binding moieties, their methods of production and selection (e.g., from diverse libraries) and their sequences are incorporated herein by reference as part of the disclosure of the present text).

Conjugation to a half-life extending moiety (e.g., albumin): In one embodiment, a (one or more) half-life extending moiety (e.g., albumin, transferrin and fragments and analogues thereof) is conjugated or associated with the TGFbetaRII-binding polypeptide, dAb or antagonist of the disclosure. Examples of suitable albumin, albumin fragments or albumin variants for use in a TGFbetaRII-binding format are described in WO2005077042, which disclosure is incorporated herein by reference and forms part of the disclosure of the present text.

Further examples of suitable albumin, fragments and analogs for use in a TGFbetaRII-binding format are described in WO 03076567, which disclosure is incorporated herein by reference and which forms part of the disclosure of the present text.

Where a (one or more) half-life extending moiety (e.g., albumin, transferrin and fragments and analogues thereof) is used to format the TGFbetaRII-binding polypeptides, dAbs and antagonists of the disclosure, it can be conjugated using any suitable method, such as, by direct fusion to the TGFbetaRII-binding moiety (e.g., anti-TGFbetaRII dAb), for example by using a single nucleotide construct that encodes a fusion protein, wherein the fusion protein is encoded as a single polypeptide chain with the half-life extending moiety located N- or C-terminally to the TGFbetaRII binding moiety. Alternatively, conjugation can be achieved by using a peptide linker between moieties, e.g., a peptide linker as described in WO03076567 or WO2004003019 (these linker disclosures being incorporated by reference in the present disclosure to provide examples for use in the present disclosure).

Conjugation to PEG: In other embodiments, the half-life extending moiety is a polyethylene glycol moiety. In one embodiment, the antagonist comprises (optionally consists of) a single variable domain of the disclosure linked to a polyethylene glycol moiety (optionally, wherein said moiety has a size of about 20 to about 50 kDa, optionally about 40 kDa linear or branched PEG). Reference is made to WO04081026 for more detail on PEGylation of dAbs and binding moieties. In one embodiment, the antagonist consists of a dAb monomer linked to a PEG, wherein the dAb monomer is a single variable domain according to the disclosure.

In another embodiment, a single variable domain, ligand or polypeptide in accordance with the disclosure may be linked to a toxin moiety or toxin.

Protease resistance: Single variable domains, polypeptides or ligands in accordance with the disclosure can be modified to improve their resistance to protease degradation. As used herein, a peptide or polypeptide (e.g. a domain antibody (dAb)) that is “resistant to protease degradation” is not substantially degraded by a protease when incubated with the protease under conditions suitable for protease activity. A polypeptide (e.g., a dAb) is not substantially degraded when no more than about 25%, no more than about 20%, no more than about 15%, no more than about 14%, no more than about 13%, no more than about 12%, no more than about 11%, no more than about 10%, no more than about 9%, no more than about 8%, no more than about 7%, no more than about 6%, no more than about 5%, no more than about 4%, no more than about 3%, no more that about 2%, no more than about 1%, or substantially none of the protein is degraded by protease after incubation with the protease for about one hour at a temperature suitable for protease activity. For example at 37 or 50 degrees C. Protein degradation can be assessed using any suitable method, for example, by SDS-PAGE or by functional assay (e.g., ligand binding) as described herein.

Methods for generating dAbs with enhanced protease resistance are disclosed, for example, in WO2008149143. In one embodiment, the single variable domain, polypeptide or ligand in accordance with the disclosure is resistant to degradation by leucozyme and/or trypsin. Polypeptides, immunoglobulin single variable domains and ligands of the disclosure may be resistant to one or more of the following: serine protease, cysteine protease, aspartate proteases, thiol proteases, matrix metalloprotease, carboxypeptidase (e.g., carboxypeptidase A, carboxypeptidase B), trypsin, chymotrypsin, pepsin, papain, elastase, leukozyme, pancreatin, thrombin, plasmin, cathepsins (e.g., cathepsin G), proteinase (e.g., proteinase 1, proteinase 2, proteinase 3), thermolysin, chymosin, enteropeptidase, caspase (e.g., caspase 1, caspase 2, caspase 4, caspase 5, caspase 9, caspase 12, caspase 13), calpain, ficain, clostripain, actimidain, bromelain, and separase. In particular embodiments, the protease is trypsin, elastase or leucozyme. The protease can also be provided by a biological extract, biological homogenate or biological preparation. Polypeptides, immunoglobulin single variable domains and ligands as disclosed herein may be selected in the presence of lung proteases, such that said polypeptides, immunoglobulin single variable domains and ligands are resistant to said lung proteases. In one embodiment, the protease is a protease found in sputum, mucus (e.g., gastric mucus, nasal mucus, bronchial mucus), bronchoalveolar lavage, lung homogenate, lung extract, pancreatic extract, gastric fluid, saliva. In one embodiment, the protease is one found in the eye and/or tears. Examples of such proteases found in the eye include caspases, calpains, matrix metalloproteases, disintegrin, metalloproteinases (e.g. ADAMs—a disintegrin and metalloproteinase) and ADAM with thrombospondin motifs, the proteosomes, tissue plasminogen activator, secretases, cathepsin B and D, cystatin C, serine protease PRSS1, ubiquitin proteosome pathway (UPP). In one embodiment, the protease is a non bacterial protease. In an embodiment, the protease is an animal, e.g., mammalian, e.g., human, protease. In an embodiment, the protease is a GI tract protease or a pulmonary tissue protease, e.g., a GI tract protease or a pulmonary tissue protease found in humans. Such protease listed here can also be used in the methods described, for example, in WO2008149143, involving exposure of a repertoire of library to a protease.

Stability: In one aspect of the disclosure, the polypeptides, single variable domains, dAbs, ligands, compositions or formulations of the disclosure are substantially stable after incubation (at a concentration of polypeptide or variable domain of 1 mg/ml) at 37 to 50° C. for 14 days in Britton Robinson or PBS buffer. In one embodiment, at least 65, 70, 75, 80, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99% of the polypeptide, antagonist or variable domain etc. remains unaggregated after such incubation at 37 degrees C. In one embodiment, at least 65, 70, 75, 80, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99% of the polypeptide or variable domain remains monomeric after such incubation at 37 degrees C.

In one embodiment, at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99% of the polypeptide, antagonist or variable domain remains unaggregated after such incubation at 50 degrees C. In one embodiment, at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99% of the polypeptide or variable domain remains monomeric after such incubation at 50 degrees C. In one embodiment, no aggregation of the polypeptides, variable domains, antagonists is seen after any one of such incubations. In one embodiment, the pI of the polypeptide or variable domain remains unchanged or substantially unchanged after incubation at 37 degrees C. at a concentration of polypeptide or variable domain of 1 mg/ml in Britton-Robinson buffer. In one aspect of the disclosure, the polypeptides, variable domains, antagonists, compositions or formulations of the disclosure are substantially stable after incubation (at a concentration of polypeptide or variable domain of 100 mg/ml) at 4° C. for 7 days in Britton Robinson buffer or PBS at a pH of 7 to 7.5 (e.g., at pH7 or pH7.5). In one embodiment, at least 95, 95.5, 96, 96.5, 97, 97.5, 98, 98.5, 99 or 99.5% of the polypeptide, antagonist or variable domain remains unaggregated after such incubation. In one embodiment, at least 95, 95.5, 96, 96.5, 97, 97.5, 98, 98.5, 99 or 99.5% of the polypeptide or variable domain remains monomeric after such incubation. In one embodiment, no aggregation of the polypeptides, variable domains, antagonists is seen after any one of such incubations.

In one aspect of the disclosure, the polypeptides, variable domains, antagonists, compositions or formulations of the disclosure are substantially stable after nebulisation (e.g. at a concentration of polypeptide or variable domain of 40 mg/ml) e.g., at room temperature, 20 degrees C. or 37° C., for 1 hour, e.g. jet nebuliser, e.g. in a Pari LC+ cup. In one embodiment, at least 65, 70, 75, 80, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 95.5, 96, 96.5, 97, 97.5, 98, 98.5, 99 or 99.5% of the polypeptide, antagonist or variable domain remains unaggregated after such nebulisation. In one embodiment, at least 65, 70, 75, 80, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 95.5, 96, 96.5, 97, 97.5, 98, 98.5, 99 or 99.5% of the polypeptide or variable domain remains monomeric after such nebulisation. In one embodiment, no aggregation of the polypeptides, variable domains, antagonists is seen after any one of such nebulisation.

Monomeric form: In one embodiment, the dAb of the present disclosure is identified to be preferentially monomeric. Suitably, the disclosure provides a (substantially) pure monomer. In one embodiment, the dAb is at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% pure or 100% pure monomer. To determine whether dAbs are monomeric or form higher order oligomers in solution, they can be analysed by SEC-MALLS. SEC MALLS (size exclusion chromatography with multi-angle-LASER-light-scattering) is a non-invasive technique for the characterizing of macromolecules in solution, that is familiar to any skilled in the art. Briefly, proteins (at concentration of 1 mg/mL in buffer Dulbecco's PBS) are separated according to their hydrodynamic properties by size exclusion chromatography (column: TSK3000; S200). Following separation, the propensity of the protein to scatter light is measured using a multi-angle-LASER-light-scattering (MALLS) detector. The intensity of the scattered light while protein passes through the detector is measured as a function of angle. This measurement taken together with the protein concentration determined using the refractive index (R1) detector allows calculation of the molar mass using appropriate equations (integral part of the analysis software Astra v.5.3.4.12).

Therapeutic use: The disclosure provides a method for treating, suppressing or preventing diseases associated with TGFbeta signaling. In one embodiment, such disease may be caused or contributed to by dysregulated TGFbeta signaling, by overexpression of TGFbeta or by high levels of bioavailable TGFbeta. Diseases associated with TGFbeta signaling include diseases relating to fibroses of various tissues, such as pulmonary fibrosis including idiopathic pulmonary fibrosis (IPF) and other interstitial lung disease such as acute respiratory distress syndrome (ARDS), fibrosis of the liver including cirrhosis and chronic hepatitis, rheumatoid arthritis, ocular disorders, vascular conditions such as restenosis, fibrosis of the skin including keloid of skin and scarring following wound healing and Dupuytren's Contracture, and kidney such as nephritis, kidney fibrosis and nephrosclerosis or a vascular condition such as restenosis. Other diseases associated with TGFbeta signaling include vascular diseases such as hypertension, pre-eclampsia, hereditary haemorrhagic telangtiectasia type I (HHT1), HHT2, pulmonary arterial hypertension, aortic aneurysms, Marfan syndrome, familial aneurysm disorder, Loeys-Dietz syndrome, arterial tortuosity syndrome (ATS). Other diseases associated with TGFbeta signaling include diseases of the musculoskeletal system such as Duchenne's muscular dystrophy and muscle fibrosis. Further diseases associated with TGFbeta signaling include cancer such as colon, gastric and pancreatic cancer as well as glioma and NSCLC. In addition, the disclosure provides methods for targeting cancer by, for example, modulating TGFbeta signaling in the tumour angiogenesis or through treatment of the cancer stroma. Other diseases or conditions include those related to tissue scarring. Other diseases include pulmonary diseases such as COPD (Chronic obstructive pulmonary disease), liver diseases such as liver failure (e.g. viral hepatitis, alcohol, obesity, autoimmune, metabolic, obstructive), kidney diseases including renal failure (e.g. diabetes, hypertension), hypertrophic cardiomyopathy, transplant rejection (lung/liver/kidney) and hypertrophic and keloid scarring.

“Fibrosis” is the result of excess deposition of extracellular matrix components such as collagen causing overgrowth, scarring and/or hardening of tissues.

“Skin Fibrosis”: cutaneous fibrosis covers a variety of human disorders with differing aetiology, but with a common dysregulation of connective tissue metabolism, particularly of dermal fibroblasts. Specific examples of cutaneous fibrosis include keloid disease, hypertrophic scars (HS) and scleroderma. Keloid disease and hypertrophic scars, although not subgroups of the same condition are both resultant from scarring following wound healing, with Keloids spreading beyond the original wound site whilst hypertrophic scar is constrained within the margins of the original wound. Scleroderma, however is used to describe fibrosis of the skin in systemic sclerosis which is a systemic condition resulting in fibrosis of multiple organs. In an embodiment, the variable domain, ligand, fusion protein or polypeptide as disclosed herein is used to prevent or treat keloid disease, hypertrophic scars or scleroderma.

“Keloids” are fibrous overgrowths at sites of cutaneous injury that form as a result of an abnormal wound-healing process in genetically susceptible individuals and, unlike normal scars, do not regress. Predominantly observed in patients with darkly pigmented skin, “Keloid disease” is a benign dermal fibroproliferative tumor unique to humans that never becomes malignant.

“Dupuytren's contracture” is a localized formation of scar tissue beneath the skin of the palm of the hand. The scarring accumulates in a tissue (fascia) that normally covers the tendons that pull the fingers to grip. As Dupuytren contracture progresses, more of the fascia becomes thickened and shortened, resulting in flexion contracture of the hand where the fingers bend towards the palm and cannot be fully extended (straightened), resulting in extreme cases to loss of use of the hand.

Scarring occurs following, surgery, injury or trauma to tissues or organs within the body. They are a consequence of repair mechanisms that generate extracellular matrix to replace missing normal tissue. The skin represents the most frequently injured tissue resulting in dermal scarring, which can result in adverse consequences including: loss of function; contracture; and, poor aesthetics which may have cause psychological effects to the sufferer. Scars can be defined ‘a macroscopic disturbance of the normal structure and function of the skin architecture, resulting from the end product of wound healing’ (Fergusson et al., 1996). Currently no therapies exist to prevent or improve scarring effectively.

The role of TGFbeta in pulmonary fibrosis has been observed (Wynn et al., J. Pathology 2008, 214, p. 199-210; Sime et al. J. Clinical Immunology 1997, Vol. 100, p. 768-776). A shift to increased production of Th2 cytokines and decreased production of Th1 cytokines is observed as a result of unknown lung injury. Overexpression of TGFbeta stimulates angiogenesis, fibroblast activation, deposition of ECM, and fibrogenesis. Animal models (e.g. TGFbeta overexpression, SMAD3 KO, inhibition of TGFbetaR signaling) show that TGFbeta is a key mediator for the development of pulmonary fibrosis.

“Idiopathic pulmonary fibrosis (IPF)” is a chronic and progressive disease resulting in abnormal and excessive deposition of fibrotic tissue in the pulmonary interstitium without a known cause. There is an incidence of approximately 10-20 cases per 100,000 in U.S per year. The prevalence increases sharply with age, reaching 175 cases per 100,000 over the age of 75 with onset usually occurring between 50 and 70 yrs. The five year survival rate is 20% with a mean survival of 2.8 years. Symptoms include a dry cough and progressive breathlessness, abnormal chest x-ray or HRCT and reduced lung volumes. Current treatments include corticosteroids (Prednisone), immunosuppressives (cyclophosphamide) or transplantation although none of the currently available therapies have a proven efficacy. In one embodiment, the single variable domain or polypeptide of the present disclosure provides a treatment for IPF.

Suitably, a successful treatment for Idiopathic pulmonary fibrosis (IPF) will show any one of a decrease in lung fibroblast proliferation, an increase in lung fibroblast apoptosis, a decrease in excessive extracellular matrix synthesis and deposition, an increase in extracellular matrix breakdown and remodelling or will show some protection against ongoing tissue injury and restoration of normal histopathology.

Suitably, a successful treatment would decelerate disease progression.

The efficacy of a treatment for IPF can be demonstrated in the bleomycin induced pulmonary fibrosis model. In one embodiment, the immunoglobulin single variable domain of the present disclosure cross reacts with mouse TGFbetaRII such that its efficacy can be tested in the mouse model.

TGFbeta is an important cell signaling molecule in the modulation of cell behaviour in ocular tissues. Overactivation of TGFbeta is implicated in the pathogenesis of fibrotic diseases in eye tissue which can be wound healing-related and lead to impaired vision and ocular tissue homeostasis (reviewed, for example, by Saika, Laboratory Investigation (2006), 86, 106-115).

Accordingly, in one embodiment, diseases associated with TGFbeta signaling include ocular disorders such as fibrotic diseases of the eye tissue. Fibrotic disease of the eye may occur in the cornea, conjunctiva, lens or retina. Ocular disorders include proliferative vitreoretinopathy (PVR), a disorder of post-retinal detachment and retinal fibrosis, diabetic retinopathy, glaucoma, such as open-angle glaucoma, angle-closure, congenital and pseudo-exfoliation syndrome, wound healing reactions in the lens, such as post chemical or thermal burn, or Stevens-Johnson's syndrome, and post-cataract surgery complications. TGFbeta also has a role in cataract development (Wormstone et al. Exp Eye Res; 83 1238-1245, 2006). A number of ocular disorders occur as a result of fibrosis post surgery. In addition, over activity of TGFbeta2 (transforming growth factor β2) is believed to cause scarring in and around the eye after glaucoma filtration surgery. TGFbeta2 is the predominant isoform involved in pathological scarring of ocular tissues including the cornea, retina, conjunctiva and trabelular meshwork. Scarring or fibrosis of the trabelular meshwork can lead to occlusion of the normal aqueous outflow pathway leading to raised intraocular pressure and risk of glaucoma development. TGFbeta 2 has been shown to be a pathological agent in pre-clinical models of glaucoma disease. TGFbeta2 levels are elevated in patients with glaucoma, in vitro treatment of huTM cells with TGFbeta-2 leads to phenotypic changes and upregulation of ECM modulating proteins (MMP-2, PAI-I) (Lutjen-Drecol (2005), Experimental Eye Research, Vol. 81, Issue 1, pages 1-4; Liton (2005), Biochemical and Biophysical Research Communications Vol. 337, issue 4, p. 1229-1236; Fuchshofer et al (2003), Experimental Eye Research, Vol. 77, issue 6, p. 757-765; Association for Research in Vision and Ophthalmology (ARVO) conference poster #1631 2009). Moreover, overexpression of TGFbeta in the eye leads to glaucoma-like pathology in mice (ARVO conference poster #5108 2009) and delivery of TGFbeta-2 using AAV has been shown to inhibit retinal ganglion cell loss in a rat model of glaucoma (ARVO conference poster #5510 2009). More recently, oxidative stress induction in cultured human optic nerve head astrocytes has been shown to increase TFGbeta2 secretion (Yu et al (2009) Invest. Ophthalmol. Vis. Sci. 50: 1707-1717). This all indicates that reduction of TGFbeta 2 levels might minimize the characteristic optic nerve head changes seen in glaucoma. However, TGFbeta is also known to have an immunosuppressive role and so in some aspects can be protective so a reduction in elevated levels of TGFbeta2 rather than a complete knock down may be preferred in treatment of chronic ocular conditions such as glaucoma. Accordingly, diseases which can be treated using the dAbs and compositions etc. in accordance with the disclosure include scarring post glaucoma filtration surgery.

Accordingly, in one aspect there is provided a method for treating, suppressing or preventing a disease associated with TGFbeta signaling and, in particular, dysregulated TGFbeta signaling, comprising administering to a mammal in need thereof a therapeutically-effective dose or amount of a polypeptide, fusion protein, single variable domain, antagonist or composition according to the disclosure.

In another aspect, the disclosure provides an immunoglobulin single variable domain, polypeptide, ligand or fusion protein in accordance with the disclosure for use as a medicament. Suitable a medicament may comprise an immunoglobulin single variable domain etc. in accordance with the disclosure formatted as described herein.

Suitably, the medicament is a pharmaceutical composition. In a further aspect of the disclosure, there is provided a composition (e.g., pharmaceutical composition) comprising a polypeptide, single variable domain, ligand, composition or antagonist according to the disclosure and a physiologically or pharmaceutically acceptable carrier, diluent or excipient. In one embodiment, the composition comprises a vehicle for delivery. In particular embodiments, the polypeptide, fusion protein, single variable domain, antagonist or composition is administered via pulmonary delivery, such as by inhalation (e.g., intrabronchial, intranasal or oral inhalation, intranasal such as by drops) or by systemic delivery (e.g., parenteral, intravenous, intramuscular, intraperitoneal, intraarterial, intrathecal, intraarticular, subcutaneous, vaginal or rectal administration). In another embodiment, the polypeptide, single variable domain, ligand or fusion protein or compositions in accordance with the disclosure is administered to the eye e.g. by topical administration, as eye drops, particulate polymer system, gel or implant, or by intraocular injection e.g. into the vitreous humour. Delivery can be targeted to particular regions of the eye such as the surface of the eye, or the tear ducts or lacrimal glands or to the anterior or posterior chambers of the eye such as the vitreous humour). It can also be useful if the immunoglobulin single variable domain, composition etc. is delivered to the eye along with an ocular penetration enhancer e.g. sodium caprate or with a viscosity enhancer e.g. Hydroxypropylmethylcellulose (HPMC). In further embodiments, the polypeptide, fusion protein, single variable domain, antagonist or composition is administered to the skin; by topical delivery to the surface of the skin and/or delivery to a region(s) within the skin e.g. intradermal delivery.

Although the most accessible organ of the body for delivery, the skin's outermost barrier, the stratum corneum (SC), acts as a rate limiting barrier for drug delivery. Traditionally, intradermal injection has been required to circumvent the SC allowing delivery of drug to site of action in deeper skin layers. Delivery however, maybe achieved through other transdermal delivery approaches. Formulation methodologies maybe utilised for delivery, including: chemical enhancers to alter the lipid structure of the SC; peptide facilitators enabling transfollicular transport; and encapsidation in particles including, liposome's, niosomes, ethosomes and transfersomes, which are believed to aid local fluidisation of the lipids and formation of depots for prolonged effect. Iontophoresis, involving the application of a small electrical potential across the skin, has been used for localised drug delivery. Iontophoresis allows for both the delivery of charged and neutral molecules by electromigration and electroosmosis respectively. Microneedles, can be employed to create micron-sized channels in the skin to overcome the SC, allowing proteins to pass through these channels to the lower epidermis. Microneedles can be broadly classified into solid and hollow microneedles. Solid microneedles, maybe used to disrupt the SC, prior to drug administration, coated to allow delivery as drug dissolves from the needles, or soluble allowing drug release as the needles dissolve in situ. Hollow microneedles allow for infusion of a liquid formulation of drug substance. Electroporation, unlike iontophoresis requires higher voltages>50V, to alter skin permeability in order to enhance drug penetration. Thermal and radiofrequency ablation methodologies allow for disruption of the SC through localised heating and ablation of the SC. In heat ablation this results following application of high temperature for short periods of time, whereas radiofrequency ablation involves use of radiofrquencies, to vibrate microelectrodes on the skin, resulting in localised heating. Disruption of the SC can also be achieved through Laser abrasion, application of low frequency ultrasound waves (sonophoresis) and jet injectors utilising high velocities to propel drug through the SC.

Moreover, the present disclosure provides a method for the treatment of disease using a polypeptide, single variable domain, composition, ligand or antagonist according to the present disclosure. In one embodiment the disease is a tissue fibrosis such as keloid disease or Dupuytren's Contracture.

In an aspect of the disclosure, the polypeptide, single variable domain, ligand, composition or antagonist is provided for therapy and/or prophylaxis of a disease or condition associated with TGFbeta signaling in a human. In another aspect, there is provided the use of the polypeptide, single variable domain, composition or antagonist, in the manufacture of a medicament for therapy or prophylaxis of a disease or condition associated with TGFbeta signaling in a human. In another aspect, there is provided a method of treating and/or preventing a disease or condition associated with TGFbeta signaling in a human patient, the method comprising administering the polypeptide, single variable domain, composition or antagonist to the patient. The disclosure also relates to therapeutic methods that comprise administering a therapeutically effective amount of a ligand of the disclosure (e.g., antagonist, or single variable domain) to a subject in need thereof.

In other embodiments, the disclosure relates to a method for treating idiopathic pulmonary fibrosis comprising administering to a subject in need thereof a therapeutically effective amount of a ligand of the disclosure (e.g., antagonist, or single variable domain).

The disclosure also relates to a drug delivery device comprising the composition (e.g., pharmaceutical composition) of the disclosure. In some embodiments, the drug delivery device comprises a plurality of therapeutically effective doses of ligand.

In other embodiments, the drug delivery device is selected from the group consisting of parenteral delivery device, intravenous delivery device, intramuscular delivery device, intraperitoneal delivery device, transdermal or intradermal delivery device, pulmonary delivery device, intraarterial delivery device, intrathecal delivery device, intraarticular delivery device, subcutaneous delivery device, intranasal delivery device, ocular delivery device, vaginal delivery device, rectal delivery device, syringe, a transdermal delivery device, an intradermal delivery device, a capsule, a tablet, a nebulizer, an inhaler, an atomizer, an aerosolizer, a mister, a dry powder inhaler, a metered dose inhaler, a metered dose sprayer, a metered dose mister, a metered dose atomizer, and a catheter. In an embodiment the drug delivery device is a transdermal or intradermal delivery device.

Suitably, the disclosure provides a pulmonary delivery device containing a polypeptide, single variable domain, composition or antagonist according to the disclosure. The device can be an inhaler or an intranasal administration device. Suitably, the pulmonary delivery device enables delivery of a therapeutically effective dose of a ligand etc. in accordance with the disclosure.

In another embodiment, the disclosure provides an ocular delivery device containing a polypeptide, single variable domain, composition or antagonist according to the disclosure. Suitably, the ocular delivery device enables delivery of a therapeutically effective dose of a ligand etc. in accordance with the disclosure.

As used herein, the term “dose” refers to the quantity of ligand administered to a subject all at one time (unit dose), or in two or more administrations over a defined time interval. For example, dose can refer to the quantity of ligand (e.g., ligand comprising an immunoglobulin single variable domain that binds TGFbetaRII) administered to a subject over the course of one day (24 hours) (daily dose), two days, one week, two weeks, three weeks or one or more months (e.g., by a single administration, or by two or more administrations). The interval between doses can be any desired amount of time. In a particular embodiment, the single variable domain or polypeptide of the invention is administered into the skin by injection, in particular by intradermal delivery, weekly or fortnightly or every 7-10 days, for example every 7, 8, 9 or 10 days.

In one embodiment, the single variable domain of the disclosure is provided as a dAb monomer, optionally unformatted (e.g., not PEGylated or half-life extended) or linked to a PEG, optionally as a dry powder formulation, optionally for delivery to a patient by inhalation (e.g., pulmonary delivery), optionally for treating and/or preventing a lung condition (e.g., Idiopathic pulmonary fibrosis).

The ligands of the disclosure provide several advantages. For example, as described herein, the ligand can be tailored to have a desired in vivo serum half-life. Domain antibodies are much smaller than conventional antibodies, and can be administered to achieve better tissue penetration than conventional antibodies. Thus, dAbs and ligands that comprise a dAb provide advantages over conventional antibodies when administered to treat disease, such as TGFbeta-signaling-mediated disease. In particular, pulmonary delivery of a dAb of the present disclosure to treat idiopathic pulmonary fibrosis enables specific local delivery of an inhibitor of TGFbeta signaling. Advantageously, an unformatted dAb monomer which specifically binds to and inhibits TGFbetaRII is small enough to be absorbed into the lung through pulmonary delivery.

The examples of WO2007085815 are incorporated herein by reference to provide details of relevant assays, formatting and experiments that can be equally applied to ligands of the present disclosure.

All publications, including but not limited to patents and patent applications, cited in this specification are herein incorporated by reference as though fully set forth.

The disclosure is further described, for the purposes of illustration only, in the following examples.

EXAMPLES Example 1 Selection of dAbs which Bind TGFbetaRII

Selection of dAbs which Bind Mouse TGFbetaRII Naïve Selections:

4G and 6G naïve phage libraries, phage libraries displaying antibody single variable domains expressed from the GAS1 leader sequence (see WO2005093074) for 4G and additionally with heat/cool preselection for 6G (see WO04101790), were used. The DOM23 leads were isolated by panning pools of VH and Vκ libraries (identified as 4G H11-19 and 6G VH2-4 (VH dAbs) and 4G κ1, 4G κ2 and 6G κ (Vκ dAbs) against the recombinant mouse and human TGF-β RII/Fc chimera protein. These chimeric proteins were made by expression of a DNA sequence encoding the amino acids residues 24 to 159 of the extracellular domain of human TGF-β Receptor Type II (Lin, et al, 1992, Cell 68:775-785) fused to the Fc region of human IgG1 in a human embryonic kidney cell line, HEK-F.

The recombinant mouse and human TGF-β RII/Fc chimera proteins were biotinylated using EZ-LINK™ Sulfo-NHS-LC-Biotin reagent (Pierce, Rockford, USA) (Henderikx, et al., 2002, Selection of antibodies against biotinylated antigens. Antibody Phage Display: Methods and protocols, Ed. O'Brien and Atkin, Humana Press). The phage libraries were pooled into six groups; 4G κ1 and κ2, 6G κ, 4G H11-13, 4G H14-16, 4G H17-19 and 6G VH2-4. 1×10¹¹ phage per library were pooled.

The phage were blocked in 2% MARVEL™ milk powder in phosphate buffered saline (MPBS) with the addition of 10 uM Human IgG Fc fragment (Native IgG Fc fragment derived from human myeloma plasma IgG, Calbiochem, California, US, cat. no. 401104) for one hour. 200 nM biotinylated mouse TGF-β RII/Fc was incubated with the blocked phage and Fc fragment mixture for 1 hour at room temperature and then captured on streptavidin DYNAbeads™ (Dynal, UK) for five minutes. The beads were washed seven times with 1 ml phosphate buffered saline/0.1% TWEEN™ (PBST), followed by a wash with 1 ml phosphate buffered saline (PBS). The biotinylated mouse TGF-β RII/Fc-bound phage were eluted in 500 ul 1 mg/ml trypsin in PBS for 10 minutes and then used to infect 1.75 ml of log-phase Escherichia coli TG1 for 30 minutes. Cells were plated on 2×TYE (Trypton Yeast Extract) agar plates supplemented with 15 ug/ml tetracycline. For subsequent rounds of selections, cells were scraped from the plates and used to inoculate 50 ml 2×TY (Trypton Yeast)+15 ug/ml tetracycline cultures that were grown overnight at 37° C. for phage amplification.

Amplified phage was recovered by centrifugation of the overnight culture for 10 minutes at 4566 g. 40 ml of supernatant containing the amplified phage was added to 10 ml of PEG/NaCl (20% v/w PEG 8000+2.5M NaCl) and incubated on ice for 45 to 60 minutes. The samples were centrifuged for 30 minutes at 4566 g to pellet the precipitated phage. The supernatant was discarded and the phage pellet was resuspended in 2 ml 15% v/v glycerol/PBS. The phage sample was transferred to 2 ml Eppendorf tubes and centrifuged for 10 minutes at g to remove any remaining bacterial cell debris. The phage was used as input phage for the second round of selection. The second round of selection was performed as described for the first round, except approximately 1×10¹⁰ phage were added, and either 200 nM human TGF-β RII/Fc or 20 nM mouse TGF-β RII/Fc was used in the selections.

Second round outputs were cloned from the fd-phage vector, pDOM4 into pDOM10. Vector pDOM4, is a derivative of the fd phage vector in which the gene III signal peptide sequence is replaced with the yeast glycolipid anchored surface protein (GAS) signal peptide. It also contains a c-myc tag between the leader sequence and gene III, which puts the gene III back in frame. This leader sequence functions well both in phage display vectors but also in other prokaryotic expression vectors and can be universally used. pDOM10 is a plasmid vector designed for soluble expression of dAbs. It is based on pUC119 vector, with expression under the control of the LacZ promoter. Expression of dAbs into the supernatant was ensured by fusion of the dAb gene to the universal GAS leader signal peptide (see WO2005093074) at the N-terminal end. In addition, a FLAG-tag was appended at the C-terminal end of the dAbs.

Subcloning of the dAb genes was performed by isolating pDOM4 DNA from the cells infected by the selected dAb-displaying fd-phage using a QIAPREP™ Spin MINIPREP™ kit in accordance with the manufacturer's instructions (cat. no. 27104, Qiagen). The DNA was amplified by PCR using biotinylated oligonucleotides D0M57 (5′ TTGCAGGCGTGGCAACAGCG-3′ (SEQ ID NO:197) and DOME (5′-CACGACGTTGTAAAACGACGGCC-3′ (SEQ ID NO:198)), digested with SalI and NotI restriction endonucleases and ligated with pDOM10 digested with SalI and NotI. The ligation products were transformed by electroporation into E coli HB2151 cells and plated on TYE plates (Trypton Yeast Extract) supplemented with 100 μg/ml of carbenicillin (TYE-carb). Individual clones were picked and expressed in overnight express auto-induction medium (high-level protein expression system, Novagen), supplemented with 100 μg/ml carbenicillin. in 96-well plates, grown with shaking at either 30° C. or 37° C. These expression plates were then centrifuged at 1800 g for 10 minutes. dAb clones that bound mouse and/or human TGF-β RII/Fc were identified by an ELISA and BIACORE™ (GE HEALTHCARE™) screen or by MSD (Meso Scale Discovery) binding assay screen. For the ELISA, 96-well Maxisorp™ immuno plates (Nunc, Denmark) were coated with either human or mouse TGF-β RII/Fc overnight at 4° C. The wells were washed three times with PBST and then blocked with 1% TWEEN™ in PBS (1% TPBS) for 1 hour at room temperature. The block was removed and a 1:1 mixture of 1% TPBS and dAb supernatant was added for 1 hour at room temperature. The plate was washed three times with PBST and the detection antibody (Monoclonal anti-FLAG M2-peroxidase antibody, Sigma-Aldrich, UK) was added and incubated for 1 hour at room temperature. The plates were developed using a colourimetric substrate (SUREBLUE™ 1-component TMB Microwell Peroxidase solution, KPL, Maryland, USA) and the optical density (OD) measured at 450 nM, the OD₄₅₀ being proportional to the amount of bound detection antibody. For BIACORE™, supernatants were diluted 1:1 in HBS-EP buffer and screened on BIACORE™ for binding to biotinylated human and mouse TGF-β RII/Fc (SA chip coated with 1500 Ru biotinylated hRII-Fc and 1550 Ru biotinylated mRII-Fc in accordance with the manufacturer's recommendations) (BIACORE™, GE HEALTHCARE™). Samples were run on BIACORE™ at a flow rate of 50 μl/min.

NaïVe Human Selections and Screening Selection of dAbs which Bind Human TGFbetaRII

Naïve selections were performed as described for mouse TGFbRII but using 150 and 15 nM biotinylated human TGFbRII/Fc at round one and two, respectively. A third round was performed using the same method as for round two, but with 1.5 nM biotinylated human TGFbRII/Fc.

The third round outputs were cloned from the fd-phage vector, pDOM4 into pDOM10. Subcloning of the dAb genes was performed by isolating pDOM4 DNA from the cells infected by the selected dAb-displaying fd-phage using a QIAPREP™ Spin MIDIPREP™ kit in accordance with the manufacturer's instructions (cat. no. 27104, Qiagen). The plasmid DNA was digested with SalI and NotI restriction endonucleases and the dAb gene insert ligated with pDOM10 digested with SalI, NotI and PstI restriction endonucleases. The ligation products were transformed by electroporation into E. coli HB2151 cells and plated on TYE plates (Trypton Yeast Extract) supplemented with 100 μg/ml of carbenicillin (TYE-carb). Individual clones were picked and expressed in 96-well plates at 250 rpm, 30° C. 72 hours, in 1 ml/well overnight express auto-induction medium (Novagen) supplemented with 100 μg/ml carbenicillin. These plates were then centrifuged at 1800 g for 10 minutes. The soluble dAb supernatants were screened for antigen binding in the TGFbRII MSD binding assay combined with the fluorescent polarization concentration determination assay. The number of human TGFbRII binders was high and there were too many clones to take forward for further characterization. Therefore, a subset of clones was sequenced and those with unique sequences were further characterized.

TGFβRII MSD Binding Assay

This assay was used to determine the binding activity of anti-TGFbRII dAbs. TGFbRII-Fc antigen was coated onto a MSD plate, which was subsequently blocked to prevent non-specific binding. Serially diluted supernatants containing soluble FLAG-tagged dAb were added. After incubation, the plate was washed and only dAbs that bound specifically to TGFBRII-Fc remained bound to the plate. Bound dAbs were detected with a ruthenylated anti-FLAG tagged antibody and MSD read buffer. If the concentration of the dAbs in the supernatant dilutions was determined using the Fluorescent Polarisation Concentration Determination assay, then concentration binding curves were plotted.

0.5 ul per well of either 60 μg/ml human TGFbRII-Fc, 60 μg/ml mouse TGFbRII-Fc or 60 ug/ml human IgG1 Fc (R&D systems, catalogue number 110-HG) was spotted onto 384 well MSD high bind plates (Meso Scale Discovery). The plates were air-dried at room temperature for a minimum of four hours and no longer than overnight. The plates were blocked with 50 ul per well of 5% MARVEL™ in Tris buffered saline (TBS)+0.1% TWEEN™ 20 for either 1 hour at room temperature or overnight at 4° C. The blocking reagent was removed from the wells by flicking the plates. A 1:3 dilution series of the dAb supernatants was prepared in 2×TY medium. The dAbs were expressed in the pDOM10 expression vector so were the dAb protein was expressed as a FLAG fusion protein. The blocking reagent was removed and 10 ul per well of the diluted dAb supernatants were transferred to the blocked MSD plates. The dAbs supernatants were screened as either 4 point curves or as 11 point curves. In addition to the diluted dAb supernatants, two controls were included in each plate, one low control (normalised to 0% binding), with no TGFbRII binding specificity and a high control (normalised to 100% binding) with high TGFbRII binding specificity, data not shown.

The plates were incubated with the dAb supernatants and the control samples for one hour at room temperature and then washed three times with 50 ul per well of TBS+0.1% TWEEN™. 15 ul/well of ruthenylated anti-FLAG antibody was added to the plates and incubated for one hour at room temperature. The anti-FLAG antibody (anti-FLAG M2 monoclonal antibody, Sigma, UK, catalogue number F3165) was conjugated to ruthenium II tris-bipyridine N-hydroxy succinimide following the manufacturer's instructions (Meso Scale Discovery, catalogue number R91BN-1). The ruthenylated anti-FLAG antibody was added to all wells except to the mouse anti-human IgG1 Fc antibody control wells. Instead, 15 ul/well of anti-Mouse MSD tag (Meso Scale Discovery, catalogue number R31AC-1) were added. The anti-Mouse MSD tag was diluted in 2% MARVEL™ in TBS+0.1% TWEEN™ 20 to a final concentration of 750 ng/ml. The plates were incubated at room temperature for one hour and washed three times with 50 ul per well of TBS+0.1% TWEEN™. 35 ul 1×MSD read buffer (Meso Scale Discovery) was added to each well and the plates were read on a MSD Sector 6000 reader (Meso Scale Discovery).

Data were analysed using XC50 Activity Base. All data was normalised to the mean of the high and low control wells on each plate, with the low control normalised to 0% binding and the high control normalised to 100% binding. A four parameter curve fit was applied to the normalised data and concentration binding curves using dAb concentrations calculated using the Fluorescent Polarisation Concentration Determination of dAbs in supernatants assay, were plotted.

The four parameter fit used was as follows:

${y = {\frac{\left( {a - d} \right)}{1 + \left( \frac{x}{c} \right)^{b}} + d}},$

where a is the minimum, b is the Hill slope, c is the XC50 and d is the maximum. Fluorescence Polarisation Concentration Determination of dAbs in Supernatants Assay

This assay allows the concentration of soluble FLAG-tagged dAbs expressed in supernatants to be determined. A fluorescently labelled-FLAG peptide was mixed with an anti-FLAG antibody. The fluorescent molecules were excited with polarised light at a wavelength of 531 nM and the emitted polarised light was read at a wavelength of 595 nM. The addition of a FLAG-tagged dAb resulted in the displacement of the fluorescent peptide from the anti-FLAG antibody which in turn resulted in reduced polarisation of the emission signal. A standard curve of known concentrations of purified FLAG-tagged VH dummy dAb was prepared and was used to back calculate the concentration of the soluble dAbs in the supernatants. The concentration data was combined with binding activity data, allowing concentration binding curves to be plotted for dAb supernatants.

The dAb supernatants were serially diluted 1:2 in 2×TY medium (1:2, 1:4, 1:8 and 1:16), followed by a 1:10 dilution in phosphate buffered saline (PBS). The diluted supernatants were transferred to a black 384 well plate. A standard curve was set up by serially diluting purified VH Dummy dAb 1:1.7 in 10% v/v 2×TY medium in PBS. The highest dAb concentration was 10 uM and there were 16 dilutions in total. 5 ul of each dilution was transferred to the 384 well plate. A mixture of 5 nM FLAG peptide labelled at the c-terminus with Cy3b, 100 mM anti-FLAG M2 monoclonal antibody (Sigma, catalogue number F3165), 0.4 mg/ml bovine serum albumin (BSA) in 2 mM CHAPs buffer was prepared. 5 ul of the mixture was transferred to the wells containing the diluted dAbs (both supernatants and standard curve wells). The plate was centrifuged at 1000 rpm (216 g) for 1 minute and then incubated in the dark at room temperature for 15 minutes. The plates were read on an ENVISION™ reader (Perkin Elmer) fitted with the following filters;

Excitation filter: BODIPY TMR FP 531 Emission filter 1: BODIPY TMR FP P pol 595 Emission filter 2: BODIPY TMR FP P pol 595

Mirror: BODIPY TMR FP Dual Enh

The standard curve was plotted and used to back calculate the concentrations of the soluble dAbs in the supernatants.

Mouse and human TGF-β RII/Fc-binding dAbs identified in the ELISA, BIACORE™ and MSD binding assays were expressed in overnight express autoinduction medium (ONEX™, Novagen) at either 30° C. for 48 to 72 hours. The cultures were centrifuged (4,600 rpm for 30 minutes) and the supernatants were incubated with STREAMLINE™-protein A beads (Amersham Biosciences, GE HEALTHCARE™, UK. Binding capacity: 5 mg of dAb per ml of beads), either overnight at 4° C. or at room temperature for at least one hour. The beads were packed into a chromatography column and washed with either 1× or 2×PBS, followed by 10 or 100 mM Tris-HCl pH 7.4 (Sigma, UK). Bound dAbs were eluted with 0.1 M glycine-HCl pH 2.0 and neutralized with 1M Tris pH 8.0. The OD at 280 nm of the dAbs was measured and protein concentrations were determined using extinction coefficients calculated from the amino acid compositions of the dAbs.

The amino acid and nucleic acid sequences of the anti-human and anti-murine TGFRII dAb naive leads are given below.

Dom23h 802 amino acid sequence (SEQ ID NO: 1) EVQLLESGGGLVQPGGSLRLSCAASGFTFSEGTMWWVRQAPGKGLEWVSAILAAGSNTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKKRQERDGFDYWGQGTLVTVSS Dom23h 802 nucleic acid sequence (SEQ ID NO: 39) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTAGTGAGGGGACGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTTTGGCTGCTGGTTCTAATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAAAAGAGGCAGGAGCGGGATGGGTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC Dom23h 803 amino acid sequence (SEQ ID NO: 2) EVQLLESGGGLVQPGGSLRLSCAASGFTFSAGRMWWVRQAPGKGLEWVSAINRDGTRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKHDDGHGNFDYWGQGTLVTVSS Dom23h 803 nucleic acid sequence (SEQ ID NO: 40) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTAGTGCTGGGCGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCGATTAATCGGGATGGTACTAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GTGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACATGATGATGGTCATGGTAAGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-813 amino acid sequence (SEQ ID NO: 3) EVQLLESGGGLVQPGGSLRLSCAASGSTFTDDRMWWVRQAPGKGLEWVSAIQPDGHTTYYADSVKGRFTISR DNSKNTLYLQMNSLRAEDTAVYYCAEQDVKGSSSFDYWGQGTLVTVSS DOM23h-813 nucleic acid sequence (SEQ ID NO: 41) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGATGATAGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTCAGCCTGATGGTCATACGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGGAACAGGATGTTAAGGGGTCGTCTTCGTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCG AGC DOM23h-815 amino acid sequence (SEQ ID NO: 4) EVQLLESGGGLVQPGGSLRLSCAASGFTFAEDRMWWVRQAPGKGLEWVSAIDPQGQHTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQSTGSATSDYWGQGTLVTVSS DOM23h-815 nucleic acid sequence (SEQ ID NO: 42) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGCGGAGGATCGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTGATCCTCAGGGTCAGCATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGTCTACTGGGTCTGCTACGTCTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-828 amino acid sequence (SEQ ID NO: 5) EVQLLESGGGLVQPGGSLRLSCAASGFTFMSYRMWWVRQAPGKGLEWVSAISPSGSDTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQVVEYSRTHKGVFDYWGQGTLVTVSS DOM23h-828 nucleic acid sequence (SEQ ID NO: 43) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTATGAGTTATAGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTTCTCCGAGTGGTAGTGATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGGTGGTGGAGTATTCGCGTACTCATAAGGGTGTGTTTGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM23h-830 amino acid sequence (SEQ ID NO: 6) EVQLLESGGGLVQPGGFLRLSCAASGFTFEGYRMWWVRQAPGKGLEWVSAIDSLGDRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQGLTHQSPSTFDYWGQGTLVTVSS DOM23h-830 nucleic acid sequence (SEQ ID NO: 44) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTTCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGAGGGGTATAGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTGATTCTCTGGGTGATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGTCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGGGGCTTACGCATCAGTCTCCGAGTACGACTACTGGGGTCAGGGAACCCTGGTCACCG TCTCGAGC DOM23h-831 amino acid sequence (SEQ ID NO: 7) EVQLLESGGGLVQPGGSLRLSCAASGFTFEAYKMTWVRQAPGKGLEWVSYITPSGGQTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKYGSSFDYWGQGTLVTVSS DOM23h-831 nucleic acid sequence (SEQ ID NO: 45) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGAGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGAGGCGTATAAGATGACGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTGGAGTGGG TCTCATATATTACGCCGTCTGGTGGTCAGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAATATGGTTCGAGGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-840 amino acid sequence (SEQ ID NO: 8) EVQLLESGGGLVQPGGSLRLSCAASGFTFGDGRMWWVRQAPGKGLEWVSAIEGAGSDTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQASRNSPFDYWGQGTLVTVSS DOM23h-840 nucleic acid sequence (SEQ ID NO: 46) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGGATGGTCGTATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTGAGGGGGCGGGTTCGGATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGGCGTCGCGGAATTCGCCGTTTGACTACTGGGGTCAGGGGACCCTGGTCACCGTCTCGAGC DOM23h-842 amino acid sequence (SEQ ID NO: 9) EVQLLESGGGLVQPGGSLRLSCAASGFTFDDSEMAWARQAPGKGLEWVSLIRRNGNATYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKVTKDRSVLFDYWGQGTLVTVSS DOM23h-842 nucleic acid sequence (SEQ ID NO: 47) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGATGATAGTGAGATGGCGTGGGCCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCACTTATTCGGCGTAATGGTAATGCTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAAGTTACGAAGGATCGTTCTGTGCTTTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGA GC DOM23h-843 amino acid sequence (SEQ ID NO: 10) EVQLLESGGGLVQPGGSLRLSCAASGFTFDQDRMWWVRQAPGKGLEWVSAIESGGHRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQNESGRSGFDYWGQGTLVTVSS DOM23h-843 nucleic acid sequence (SEQ ID NO: 48) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGATCAGGATCGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTGAGAGTGGTGGTCATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGAATGAGTCGGGGCGTTCGGGGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCG AGC DOM23h-850 amino acid sequence (SEQ ID NO: 11) EVQLLESGGGLVQPGGSLRLSCAASGFTFDAARMWWARQAPGKGLEWVSAIADIGNTTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQSGSEDHFDYWGQGTLVTVSS DOM23h-850 nucleic acid sequence (SEQ ID NO: 49) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGATGCGGCTAGGATGTGGTGGGCCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCGATTGCGGATATTGGTAATACTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGTCTGGTTCGGAGGATCAGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-854 amino acid sequence (SEQ ID NO: 12) EVQLLESGGGLVQPGGSLRLSCAASGFTFAQDRMWWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQDLHGTSSLFDYWGQGTLVTVSS DOM23h-854 nucleic acid sequence (SEQ ID NO: 50) GAGGTGCAGCTGTTGGAGTCCGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGCTCAGGATCGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTAGTGGTAGTGGTGGTAGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGGATTTGCATGGTACTAGTTCTTTGTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCT CGAGC DOM23h-855 amino acid sequence (SEQ ID NO: 13) EVQLLESGGGLVQPGGSLRLSCAASGFTFENTSMGWVRQAPGKGLEWVSRIDPKGSHTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQRELGKSHFDYWGQGTLVTVSS DOM23h-855 nucleic acid sequence (SEQ ID NO: 51) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGAGAATACGAGTATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCACGTATTGATCCTAAGGGTAGTCATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAATACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACAGCGTGAGTTGGGTAAGTCGCAGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCG AGC DOM23h-865 amino acid sequence (SEQ ID NO: 14) EVQLLESGGGLVQPGGSLRLSCAASGFTFRSYEMTWVRQAPGKGLEWVSKIDPSGRFTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKGRTDLQLFDYWGQGTLVTVSS DOM23h-865 nucleic acid sequence (SEQ ID NO: 52) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTCGTAGTTATGAGATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGGT CTCAAAGATTGATCCTTCGGGTCGIIIIACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACTG TGCGAAAGGTCGGACGGATCTTCAGCTTTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-866 amino acid sequence (SEQ ID NO: 15) EVQLLESGGGLVQPGGSLRLSCAASGFTFSNYWMRWARQAPGKGLEWVSYITPKGDHTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAESLHNERVKHFDYWGQGTLVTVSS DOM23h-866 nucleic acid sequence (SEQ ID NO: 53) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCIIIICGAATTATTGGATGCGGTGGGCCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCATATATTACTCCTAAGGGTGATCATACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTG TGCGGAATCGCTTCATAATGAGCGTGTTAAGCA GACTACTGGGGTCAGGGAACCCTGGTCACCGTCTC GAGC DOM23h-874 amino acid sequence (SEQ ID NO: 16) EVQLLESGGGLVQPGGSLRLSCAASGFTFTSYRMWWVRQAPGKGLEWVSVIDSTGSATYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQQAGSAMGEFDYWGQGTLVTVSS DOM23h-874 nucleic acid sequence (SEQ ID NO: 54) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACTAGTTATCGTATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGGT CTCAGTTATTGATTCTACTGGTTCGGCTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACTG TGCGAAACAGCAGGCTGGGAGTGCGATGGGGGAGTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCT CGAGC DOM23h-883 amino acid sequence (SEQ ID NO: 17) EVQLLESGGGLVQPGGSLRLSCAASGFTFVNYRMWWVRQAPGKGLEWVSAISGSGDKTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKHGLSFDYWGQGTLVTVSS DOM23h-883 nucleic acid sequence (SEQ ID NO: 55) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGTTAATTATCGTATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGGT CTCAGCTATTAGTGGTAGTGGTGATAAGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTG TGCGAAACATGGGCTGTCGTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-903 amino acid sequence (SEQ ID NO: 18) EVQLLESGGGLVQPGGSLRLSCAASGFTFNDMRMWWVRQAPGKGLEWVSVINADGNRTYYADSVKGRFTISR DNSKNTLYLQMNSLRAEDTAVYYCAKDGLPFDYWGQGTLVTVSS DOM23h-903 nucleic acid sequence (SEQ ID NO: 56) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTAATGATATGAGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGTGATTAATGCTGATGGTAATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAAGATGGGCTGCCGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23m-4 amino acid sequence (SEQ ID NO: 19) EVQLLESGGGLVQPGGSLRLSCAASGFTFTTYGMGWVRQAPGKGLEWVSWIEKTGNKMADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKAGRHIKVRSRDFDYWGQGTLVTVSS DOM23m-4 nucleic acid sequence (SEQ ID NO: 57) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGACTTATGGTATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCATGGATTGAGAAGACGGGTAATAAGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAAGCGGGGAGGCATATTAAGGTGCGTTCGAGGGAGACTACTGGGGTCAGGGAACCCTGGTC ACCGTCTCGAGC DOM23m-29 amino acid sequence (SEQ ID NO: 20) EVQLLESGGGLVQPGGSLRLSCAASGFTFKRYSMGWVRQAPGKGLEWVSVINDLGSLTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKGNISMVRPGSWFDYWGQGTLVTVSS DOM23m-29 nucleic acid sequence (SEQ ID NO: 58) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTAAGAGGTATTCTATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGTTATTAATGATCTGGGTAGTTTGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAAGGGAATATTAGTATGGTGAGGCCGGGGAGTTGGTTTGACTACTGGGGTCAGGGAACCCTGGTC ACCGTCTCGAGC DOM23m-32 amino acid sequence (SEQ ID NO: 21) EVQLLESGGGLVQPGGSLRLSCAASGFTFFEYPMGWVRQAPGKGLEWVSVISGDGQRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKSHTGTVRHLETFDYWGQGTLVTVSS DOM23m-32 nucleic acid sequence (SEQ ID NO: 59) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTTTTGAGTATCCTATGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGGT CTCAGTTATTAGTGGGGATGGTCAGCGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTG TGCGAAAAGTCATACGGGGACTGTGAGGCATCTGGAGACGTTTGACTACTGGGGTCAGGGAACCCTGGTCA CCGTCTCGAGC DOM23m-62 amino acid sequence (SEQ ID NO: 22) EVQLLESGGGLVQPGGSLRLSCAASGFTFGQESMYWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKSGTRIKQGFDYWGQGTLVTVSS DOM23m-62 nucleic acid sequence (SEQ ID NO: 60) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGTCAGGAGAGTATGTATTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTAGTGGTAGTGGTGGTAGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAAAGTGGTACGCGGATTAAGCAGGGGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCG AGC DOM23m-71 amino acid sequence (SEQ ID NO: 23) EVQLLESGGGLVQPGGSLRLSCAASGFTFMDYRMYWVRQAPGKGLEWVSGIDPTGLRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKIKWGEMGSYKTFDYWGQGTLVTVSS DOM23m-71 nucleic acid sequence (SEQ ID NO: 61) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTATGGATTATAGGATGTATTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGGT CTCAGGGATTGATCCTACTGGTTTGCGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTG TGCGAAAATTAAGTGGGGGGAGATGGGGAGTTATAAGACGACTACTGGGGTCAGGGAACCCTGGTCA CCGTCTCGAGC DOM23m-72 amino acid sequence (SEQ ID NO: 24) EVQLLESGGGLVQPGGSLRLSCAASGFTFMDYDMSWVRQAPGKGLEWVSMIREDGGKTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKARVPYRRGHRDNFDYWGQGTLVTVSS DOM23m-72 nucleic acid sequence (SEQ ID NO: 62) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTATGGATTATGATATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGGT CTCAATGATTCGTGAGGATGGTGGTAAGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTG TGCGAAAGCGAGGGTGCCTTATCGGCGTGGGCATAGGGATAAGACTACTGGGGTCAGGGAACCCTGG TCACCGTCTCGAGC DOM23m-81 amino acid sequence (SEQ ID NO: 25) EVQLLESGGGLVQPGGSLRLSCAASGFTFEPVIMGWVRQAPGKGLEWVSAIEARGGGTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKPGRHLSQDFDYWGQGTLVTVSS DOM23m-81 nucleic acid sequence (SEQ ID NO: 63) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC TTCCGGATTCACCTTTGAGCCGGTTATTATGGGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAGCTATTGAGGCGCGGGGTGGGGGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCC CGCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTAC TGTGCGAAACCTGGGCGGCATCTTAGTCAGGAGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCG AGC DOM23m-99 amino acid sequence (SEQ ID NO: 26) EVQLLESGGGLVQPGGSLRLSCAASGFTFDRYRMMWVRQAPGKGLEWVSTIDPAGMLTY YADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKRLASRSHFDYWGQGTLVTVSS DOM23m-99 nucleic acid sequence (SEQ ID NO: 64) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGATCGGTATCGTATGATGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTGGG TCTCAACGATTGATCCTGCTGGTATGCTTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAAAGGCTGGCTTCGCGGAGTCAGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23m-101 amino acid sequence (SEQ ID NO: 27) EVQLLESGGGLVQPGGSLRLSCAASGFTFSEYDMAWVRQAPGKGLEWVSRIRSDGVRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKDRAKNGWFDYWGQGTLVTVSS DOM23m-101 nucleic acid sequence (SEQ ID NO: 65) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGCGCAGC CTCCGGATTCACCIIIICTGAGTATGATATGGCTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTTGAGTGGGT CTCACGGATTCGTTCTGATGGTGTTAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTG TGCGAAAGATCGTGCTAAGAATGGTTGGTTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-352 amino acid sequence (SEQ ID NO: 28) EVQLLESGGGLVQPGGSLRLSCAASGFTFDKYKMAWVRQAPGKGLEWVSLIFPNGVPTYYANSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKYSGQGRDFDYWGQGTLVTVSS DOM23h-352 nucleic acid sequence (SEQ ID NO: 66) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGATAAGTATAAGATGGCTTGGGTCCGCCAGGCTCCAGGGAAGGGTCTGGAGTGGG TCTCACTTATTTTTCCGAATGGTGTTCCTACATACTACGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCCG CGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTG TGCGAAATATAGTGGTCAGGGGCGGGAGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC

The CDRs as defined by Kabat of these anti-human and anti-murine TGFRII dAb naive leads are shown in Tables 1 and 2, below, respectively.

TABLE 1  CDR Sequences of anti-human TGFβRII dAbs Clone CDR1 CDR2 CDR3 DOM23h-802 SEGTMW AILAAGSNTYYADSVKG KRQERDGFDY (SEQ ID NO: 77) (SEQ ID NO: 113) (SEQ ID NO: 149) DOM23h-803 SAGRMW AINRDGTRTYYADSVKG HDDGHGNFDY (SEQ ID NO: 78) (SEQ ID NO: 114) (SEQ ID NO: 150) DOM23h-813 TDDRMW AIQPDGHTTYYADSVKG EQDVKGSSSFDY (SEQ ID NO: 79) (SEQ ID NO: 115) (SEQ ID NO: 151) DOM23h-815 AEDRMW AIDPQGQHTYYADSVKG QSTGSATSDY (SEQ ID NO: 80) (SEQ ID NO: 116) (SEQ ID NO: 152) DOM23h-828 MSYRMW AISPSGSDTYYADSVKG QVVEYSRTHKGVFDY (SEQ ID NO: 81) (SEQ ID NO: 117) (SEQ ID NO: 153) DOM23h-830 EGYRMW AIDSLGDRTYYADSVKG QGLTHQSPSTFDY (SEQ ID NO: 82) (SEQ ID NO: 118) (SEQ ID NO: 154) DOM23h-831 EAYKMT YITPSGGQTYYADSVKG YGSSFDY (SEQ ID NO: 83) (SEQ ID NO: 119) (SEQ ID NO: 155) DOM23h-840 GDGRMW AIEGAGSDTYYADSVKG QASRNSPFDY (SEQ ID NO: 84) (SEQ ID NO: 120) (SEQ ID NO: 156) DOM23h-842 DDSEMA LIRRNGNATYYADSVKG VTKDRSVLFDY (SEQ ID NO: 85) (SEQ ID NO: 121) (SEQ ID NO: 157) DOM23h-843 DQDRMW AIESGGHRTYYADSVKG QNESGRSGFDY (SEQ ID NO: 86) (SEQ ID NO: 122) (SEQ ID NO: 158) DOM23h-850 DAARMW AIADIGNTTYYADSVKG QSGSEDHFDY (SEQ ID NO: 87) (SEQ ID NO: 123) (SEQ ID NO: 159) DOM23h-854 AQDRMW AISGSGGSTYYADSVKG QDLHGTSSLFDY (SEQ ID NO: 88) (SEQ ID NO: 124) (SEQ ID NO: 160) DOM23h-855 ENTSMG RIDPKGSHTYYADSVKG QRELGKSHFDY (SEQ ID NO: 89) (SEQ ID NO: 125) (SEQ ID NO: 161) DOM23h-865 RSYEMT KIDPSGRFTYYADSVKG GRTDLQLFDY (SEQ ID NO: 90) (SEQ ID NO: 126) (SEQ ID NO: 162) DOM23h-866 SNYWMR YITPKGDHTYYADSVKG SLHNERVKHFDY (SEQ ID NO: 91) (SEQ ID NO: 127) (SEQ ID NO: 163) DOM23h-874 TSYRMW VIDSTGSATYYADSVKG QQAGSAMGEFDY (SEQ ID NO: 92) (SEQ ID NO: 128) (SEQ ID NO: 164) DOM23h-883 VNYRMW AISGSGDKTYYADSVKG HGLSFDY (SEQ ID NO: 93) (SEQ ID NO: 129) (SEQ ID NO: 165) DOM23h-903 NDMRMW VINADGNRTYYADSVKG DGLPFDY (SEQ ID NO: 94) (SEQ ID NO: 130) (SEQ ID NO: 166)

TABLE 2  CDR Sequences of anti-murine TGFβRII dAbs Clone CDR1 CDR2 CDR3 DOM23m-4 TTYGMG WIEKTGNKTYYADSVKG AGRHIKVRSRDFDY (SEQ ID NO: 95) (SEQ ID NO: 131) (SEQ ID NO: 167) DOM23m-29 KRYSMG VINDLGSLTYYADSVKG GNISMVRPGSWFDY (SEQ ID NO: 96) (SEQ ID NO: 132) (SEQ ID NO: 168) DOM23m-32 FEYPMG VISGDGQRTYYADSVKG SHTGTVRHLETFDY (SEQ ID NO: 97) (SEQ ID NO: 133) (SEQ ID NO: 169) DOM23m-62 GQESMY AISGSGGSTYYADSVKGR SGTRIKQGFDY (SEQ ID NO: 98) (SEQ ID NO: 134) (SEQ ID NO: 170) DOM23m-71 MDYRMY GIDPTGLRTYYADSVKG IKWGEMGSYKTFDY (SEQ ID NO: 99) (SEQ ID NO: 135) (SEQ ID NO: 171) DOM23m-72 MDYDMS MIREDGGKTYYADSVKGR ARVPYRRGHRDNFDY (SEQ ID NO: 100) (SEQ ID NO: 136) (SEQ ID NO: 172) DOM23m-81 EPVIMG AIEARGGGTYYADSVKG PGRHLSQDFDY (SEQ ID NO: 101) (SEQ ID NO: 137) (SEQ ID NO: 173) DOM23m-99 DRYRMM TIDPAGMLTYYADSVKG RLASRSHFDY (SEQ ID NO: 102) (SEQ ID NO: 138) (SEQ ID NO: 174) DOM23m-101 SEYDMA RIRSDGVRTYYADSVKG DRAKNGWFDY (SEQ ID NO: 103) (SEQ ID NO: 139) (SEQ ID NO: 175) DOM23h-352 DKYKMA LIFPNGVPTYYANSVKG YSGQGRDFDY (SEQ ID NO: 104) (SEQ ID NO: 140) (SEQ ID NO: 176)

Example 2 DSC (Differential Scanning Calorimetry)—Naive Clones

dAbs thermal stability was determined using Differential Scanning calorimetry (DSC). dAbs were dialysed overnight into PBS to a final concentration of 1 mg/ml. The dialysis buffer was used as a reference for all samples. DSC measurements were performed using the GE HEALTHCARE™-MICROCAL™VP-DSC capillary cell microcalorimeter, at a heating rate of 180° C./hour. A typical scan range was from 20-90° C. for both the reference buffer and the protein sample. A rescan was performed each time in order to assess the extent of protein refolding under these experimental conditions. After each protein sample scan, the capillary cell was cleaned with a solution of 5% DECON™ (Fisher-Scientific) in water followed by a PBS scan. Resulting data traces were analyzed using Origin 7.0 software. The DSC trace obtained from the reference buffer scan was subtracted from that of the protein sample scan. The precise molar concentration of the protein sample was entered into the data analysis routine to yield values for melting temperature (Tm), enthalpy (ΔH) and Van't Hoff enthalpy (ΔHv) values. Data were fitted to a non-2-state model (N2M). The best fit was obtained with either 1 or 2 transition events. The Tm values obtained for the dAbs described in this patent range from 52.1° C. to 73.3° C. Tm values and percentage of refolding are shown in Table 3.

TABLE 3 DSC Apparent Tm ° C. 1-transition N2M 2-transition N2M dAb Name Tm Tm1 Tm2 % refolding DOM23h-802 — 56.28 57.54 0 DOM23h-803 — 61.19 64.59 23 DOM23h-813 52.11 — — 100 DOM23h-815 65.13 — — 93 DOM23h-828 — 60.86 59.40 0 DOM23h-830 — 57.01 58.15 0 DOM23h-831 — 55.29 57.19 0 DOM23h-840 63.70 — — 100 DOM23h-842 63.08 — — 27 DOM23h-843 60.15 — — 60 DOM23h-850 58.27 — — 60 DOM23h-854 — 55.31 58.20 30 DOM23h-855 70.32 — — 88 DOM23h-865 63.02 — — 0 DOM23h-866 — 52.88 55.77 18 DOM23h-874 — 58.83 60.15 0 DOM23h-883 — 66.78 59.14 0 DOM23h-903 — 59.11 61.98 24 DOM23m-4 — 57.1  61.3  0 DOM23m-29 68   — — 0 DOM23m-32 — 70.4  73.3  25 DOM23m-62 — — — — DOM23m-71 63   — — 0 DOM23m-72 — — — — DOM23m-81 — — — — DOM23m-99 — 58.5  59   0 DOM23m-101 64   — — 30 DOM23m-352 66   — — 50

All molecules maintain tertiary structure up to at least 52° C. upon heating.

Example 3 SEC-MALS (Size Exclusion Chromatography with Multi-Angle-LASER-Light Scattering)—Naive Clones

To determine whether dAbs are monomeric or form higher order oligomers in solution, they were analyzed by SEC-MALLS (Size Exclusion Chromatography with Multi-Angle-LASER-Light-Scattering). Agilent 1100 series HPLC system with an autosampler and a UV detector (controlled by Empower software) was connected to Wyatt Mini Dawn Treos (Laser Light Scattering (LS) detector) and Wyatt Optilab rEX DRI (Differential Refractive Index (R1) detector). The detectors were connected in the following order -UV-LS-R1. Both RI and LS instruments operate at a wavelength of 658 nm; the UV signal was monitored at 280 nm and 220 nm. Domain antibodies (100 microliters injection at a concentration of 1 mg/mL in PBS) were separated according to their hydrodynamic properties by size exclusion chromatography using a GE HEALTHCARE™ 10/300 Superdex 75 column. The mobile phase was PBS plus 10% ethanol. The intensity of the scattered light while protein passed through the detector was measured as a function of angle. This measurement taken together with the protein concentration determined using the RI detector allowed calculation of the molar mass using appropriate equations (integral part of the analysis software Astra v.5.3.4.14). All the dAbs described herein have a monomeric content ranging from 65% to 98%. Data is shown in Table 4.

TABLE 4 dAb name Monomer by SEC-MALLS (%) DOM23h-802 92.5 DOM23h-803 96.4 DOM23h-813 96.6 DOM23h-815 98 DOM23h-828 80 DOM23h-830 65 DOM23h-831 72 DOM23h-840 91 DOM23h-842 91.6 DOM23h-843 90.2 DOM23h-850 97.7 DOM23h-854 83.4 DOM23h-855 96.3 DOM23h-865 83 DOM23h-866 92.4 DOM23h-874 92.6 DOM23h-883 93.5 DOM23h-903 96.5 DOM23m-4* 93 DOM23m-29* 95 DOM23m-32* 92 DOM23m-62 Not determined DOM23m-71* 88 DOM23m-72 Not determined DOM23m-81 Not determined DOM23m-99 79 DOM23m-101 77.4 DOM23m-352 93 *These dAbs were run using the same SEC-MALLS set up as described above except that the HPLC used was a Shimadzu LC-20AD Prominence system. These dAbs were also run on a Superdex75 column but the mobile phase buffer was PBS.

The molecules listed in the tables 3 and 4 were chosen on the basis of Solution State (propensity for monomer) content and Thermal stability. All molecules show a ≧65% propensity for monomerisation and maintain tertiary structure up to at least 52° C. upon heating.

Example 4 Assays for TGFbetaRII Inhibition (Naive Clones) MC3T3-E1 Luciferase Assay—Method m1:

The MC3T3-E1 luciferase assay measures the ability of dAbs to inhibit TGFβ-induced expression of CAGA-luciferase in MC3T3-E1 cells. Three copies of a TGFβ-responsive sequence motif, termed a CAGA box are present in the human PAI-1 promoter and specifically bind Smad3 and 4 proteins. Cloning multiple copies of the CAGA box into a luciferase reporter construct confers TGFβ responsiveness to cells transfected with the reporter system. This assay uses MC3T3-E1 cells (mouse osteoblasts) stably transfected with a [CAGA]₁₂-luciferase reporter construct (Dennler, et al. (1998) EMBO J. 17, 3091-3100).

Soluble dAbs were tested for their ability to block TGF-β1 signaling via the Smad3/4 pathway.

The protocol used to generate the data which appears as method m1 in table 5, is as follows. Briefly, 2.5×10⁴ MC3T3-E1 cells per well in assay medium (RPMI medium (Gibco, Invitrogen Ltd, Paisley, UK), 10% heat inactivated foetal calf serum, and 1% penicillin/streptomycin) were added to a tissue culture 96 well plate (Nunc), followed by the dAb and TGF-β1 (final concentration 1 ng/ml) and incubated for six hours at 37° C., 5% CO₂. dAbs were dialysed into PBS prior to being tested in the assay. BRIGHTGLOW™ luciferase reagent (Promega, UK) was added to the wells and incubated at room temperature for two minutes to allow the cells to lyse, and the resulting luminescence measured on a luminometer.

The assay was performed multiple times to obtain an average and range of maximum % inhibitions values which are summarised in Table 5. This method has been modified and is described Below.

Modified MC3T3-E1 Luciferase Assay—Method m2.

MC3T3-E1 cells were added to 96 well plates (Nunc 13610) at 1.25×10⁴ per well in “plating medium” (MEM-Alpha+Ribonucleosides, +Deoxyribonucleosides (Invitrogen 22571), 5% Charcoal stripped FCS (Perbio Sciences UK Ltd; SH30068.03), 1/100 Sodium Pyruvate (Invitrogen11360), 250 μg/ml of Geneticin 50 mg/ml (Invitrogen, 10131027), and incubated overnight at 37° C., 5% CO₂. The media from the cells was replaced with “assay media” (DMEM (Invitrogen 31966021,) 25 mM Hepes (Invitrogen)), and purified dAbs in PBS at 4× final assay concentration were titrated in “assay media” and added to the cell plates, followed by TGF-β1 (R&D, 240B) at 4× the EC80. The plates were incubated for six hours at 37° C., 5% CO₂. STEADYLITE™ luciferase reagent (PerkinElmer 6016987) was added to the wells and incubated at room temperature for 30 minutes, and the resulting luminescence measured on a the ENVISION™ plate reader.

Each dAb was titrated in duplicate in an assay and a maximum % inhibition determined (n=2). The assay was performed multiple times to obtain an average and range of maximum % inhibitions values which are summarised in Table 5. The assay QC parameters were met; in-house small molecule showing an IC50 range from 100 to 900 nM for the mouse assays. Also, the robust Z factors were greater than 0.4 and the TGF-β EC80 was within 6 fold of the concentration added to the assay.

A549 IL-11 Release Assay—h1

The A549 Interleukin-11 (IL-11) release assay measures the ability of dAbs to inhibit human TGF-β1 stimulated IL-11 release from A549 cells. TGF-β1 binds directly to TGF-βRII and induces the assembly of the TGF-βR1/II complex. TGF-βRI is phosphorylated and is able to signal through several pathways including the Smad4 pathway. Activation of the Smad4 pathway results in the release of IL-11. The IL-11 is secreted into the cell supernatant and is then measured by colourmetric ELISA.

Soluble dAbs were tested for their ability to block TGF-β1 signalling via the Smad4 pathway. Briefly, 1×10⁵ A549 cells per well in “assay medium” (DMEM high glucose medium (Gibco™, Invitrogen Ltd, Paisley, UK), 10% heat inactivated foetal calf serum (PAA, Austria), 10 mM HEPES (Sigma, UK) and 1% penicillin/streptomycin (PAA, Austria)) were added to a tissue culture 96 well plate (Nunc), followed by the dAb and TGF-β1 (final concentration 3 ng/ml) (R&D Systems, Abingdon, UK) and incubated overnight at 37° C., 5% CO₂. dAbs were dialysed into PBS prior to being assayed. The concentration of IL-11 released into the supernatant was measured using a Human IL-11 DUOSET™ (R&D systems, Abingdon, UK), in accordance with the manufacturer's instructions.

The A549 IL-11 release assay is referred to in tables 5 and 6 as assay method h1. The assay was performed multiple times to obtain an average and range of maximum % inhibitions values which are summarised in Table 5. The assay QC parameters were met; in-house small molecule showing an IC50 range from 50 to 500 nM for the human assays.

SBE-bla HEK 293T Cell Sensor Assay—h2:

Members of the Smad family of signal transduction molecules are components of an intracellular pathway that transmits TGF-β signals from the cell surface to nucleus. TGF-β1 binds directly to TGF-βRII and induces the assembly of the TGF-βRI/II complex. Smad2 and Smad3 are then phosphorylated by TGF-βRI, and subsequently form a heteromeric complex with the co-smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription.

Cell Sensor SBE-bla HEK 293T cells contain a beta-lactamase reporter gene under control of the Smad binding element (SBE) which was stably integrated into HEK 293T cells (Invitrogen, UK). The cells are responsive to TGF-βI and can be used to detect agonists/antagonists of the Smad2/3 signaling pathway.

Soluble dAbs were tested for their ability to block TGF-β1 signaling via this pathway following the method below, which was based on an optimised method from Invitrogen, UK, (cell line K1108).

The assay was performed direct from frozen cells which had been grown for at least 4 passages in growth media (DMEM high glucose, Invitrogen 21068028, 10% Dialysed U.S. FBS. Invitrogen 26400-044, 0.1 mM (1/100) Non essential amino acids. Invitrogen 11140-050, 25 mM (1/40) HEPES buffer. Sigma H0887, 1 mM (1/100) Sodium pyruvate. Invitrogen 11360-070, 1% GLUTAMAX™. (200 mM Invitrogen 35050038), 5 μg/ml of Blasticidin. Invitrogen R21001) and frozen in house (at 4×10⁷/ml). The cells were plated at 20,000 cells per well in cell culture plates (Costar 3712) in plating media (as above with 1% FCS and no blasticidin). After incubating the cells overnight, the purified dAbs were diluted in “assay media” (DMEM (Invitrogen 31966021,) 25 mM Hepes (Invitrogen) and added to the cells at 4× final assay concentration. After a 1 hour incubation at 37° C., TGF-β (R&D Systems; 240B) was added at 4×EC80 and incubated for a further 5 hours. The LIVEBLAZER™ substrate (Invitrogen K1030), was made up according to the manufacturer's instructions and added at 8× the volume. The plates were incubated in the dark at room temperature for 16 hours and read on the ENVISION™ plate reader according to the Invitrogen protocol.

The SBE-bla HEK CELLSENSOR™ assay is referred to in tables 5 and 6 as method h2. Each dAb was titrated in duplicate in an assay and an IC50 determined and maximum % inhibition determined (n=2). Due to the difficulty of obtaining full curves in the mouse assay, only % inhibitions are quoted in table 5. The assay was performed multiple times to obtain an average and a range of values which are summarised in Tables 5 and 6. The arithmetric mean IC50 was calculated using pIC50's (-log of IC50), and the range calculated adding and subtracting the log standard deviation from mean pIC50, and then transforming back to IC50. The assay QC parameters were met; in-house small molecule showing an IC50 range from 50 to 500 nM for the human assays. Also, the robust Z factors were greater than 0.4 and the TGF-β EC80 was within 6 fold of the concentration added to the assay

The results are shown in Tables 5 and 6.

TABLE 5 Cell Functional assay data for mouse specific clones plus VH Dummy dAb. Human IL-11 release (h1) or SBE-bla HEK Mouse 3T3 cell assay CELLSENSOR ™ assay (h2) Assay max % inhibition Assay max % inhibition Method Average SD range n Method Average SD range n DOM23m-04 m1 73.3 8.4 68.8-83   3 h1 70.7 6.4 67-78 3 DOM23m-04 h2 69.0 1 DOM23m-29 m1 54.2 5.2 50.5-57.9 2 DOM23m-32 m1 39.7 4.0 36.9-42.5 2 DOM23m-62 m1 78.6 1 h1 79.0 1 DOM23m-71 m1 44.9 9.3 38.3-51.4 2 h1 −2.0 1 DOM23m-72 m1 17.5 24.7   0-34.9 2 h1 1.7 1 DOM23m-81 m2 30.3 11.5 21-47 4 DOM23m-99 m2 46.5 28.0 26.7-93.5 6 DOM23m-101 m2 48.0 19 22.0-74.1 12 h2 59.8 27.0 17-81 5 DOM23h-352 m2 48.0 23.9 16.8-78.9 16 h2 46.7 36.5 5.7-86  5 VHDUM-2 m2 21.4 13.2   21-33.9 15 h2 46.0 29.6 17-84 6 VHDUM-2 m1 22.5 0 22.5 2

TABLE 6 Cell Functional data for human specific clones plus VH Dummy dAb. IC50 nM Assay IC50 range method dAb Mean (+/− log SD) n h2 DOM23h-802 >11062 6592-18562 6 h2 DOM23h-803 >11619 5890-22922 6 h2 DOM23h-813 >9328 4301-20230 6 h2 DOM23h-815 7122 3026-16764 4 h2 DOM23h-828 9899.07 4441-22065 4 h2 DOM23h 830 6299 5442-7291  4 h2 DOM23h-831 >3126  534-18291 8 h2 DOM23h 840 2915  650-13081 7 h2 DOM23h 842 2042 2223-18704 4 h2 DOM23h-843 >9007 3396-23894 8 h2 DOM23h-850 5350 2358-12137 6 h2 DOM23h-854 >9551 3085-29569 8 h2 DOM23h-855 >4467 1088-18339 8 h2 DOM23h 865 5559 1070-28893 4 h2 DOM23h 866 >1762  195-15900 6 h2 DOM23h 874 >925  89-9591 6 h2 DOM23h 883 10123  60-17344 6 h2 DOM23h 903 1048 492-223  5 h2 VHDummy-2 >25119 25000-50000  12

The mouse clones were selected on the basis that they showed greater than 40% neutralisation of TGF-β in several assays. The only exception to this was DOM23m-72. The clones also showed good neutralisation curves (data not shown). The human clones were selected on the basis that the average IC50's were less than 15 μM.

Example 5 Error Prone Affinity Maturation of Naive Clones (from Example 1)

Error-prone mutagenesis was performed to improve the affinity of the dAbs identified as active with suitable biophysical characteristics (described above).

Phage Library Construction: Error prone libraries of DOM23h-843, DOM23h-850, DOM23h-854, DOM23h-855, DOM23h-865, DOM23h-866, DOM23h-874, DOM23h-883, DOM23h-439 and DOM23h-903, were made using GENEMORPH™ II Random Mutagenesis kit (Stratagene, Cat No 200550). The target dAb genes were amplified by PCR using Taq DNA polymerase and oligonucleotides DOM008 (5′-AGCGGATAACAATTTCACACAGGA-3′ (SEQ ID NO:185)) and DOM009 (5′-CGCCAGGGTTTTCCCAGTCACGAC-3′ (SEQ ID NO:186)), followed by re-amplification of the diluted PCR product with oligonucleotides D0M172 (5′ TTGCAGGCGTGGCAACAGCG-3′ (SEQ ID NO:187)) and D0M173 (5f-CACGACGTTGTAAAACGACGGCC-3′ (SEQ ID NO:188)), and MUTAZYME™ II DNA polymerase, according to manufacturer's instructions. This PCR product was further amplified using Taq DNA polymerase and oligonucleotides DOM172 and DOM173, to increase the DNA product yield. The PCR product was digested with Sal I and Not I restriction endonucleases. Undigested product and digested ends were removed from the digested product using streptavidin beads (Dynal Biotech, UK). For the anti-human error prone selections digested product was ligated into pDOM4 phage vector digested with Sal I and Not I restriction endonucleases and used to transform E. coli TB1 cells. The transformed cells were plated on 2×TY agar supplemented with 15 μg/ml tetracycline, yielding library sizes of >1×10⁷ transformants.

Human TGFbetaRII specific dAb Error-prone selections: Three rounds of selection were performed with the DOM23h-843, DOM23h-850, DOM23h-854, DOM23h-855, DOM23h-865, DOM23h-866, DOM23h-874, DOM23h-883, DOM23h-903, and DOM23h-439 libraries. Round one was performed using 1 nM biotinylated human TGFbetaRII/Fc (N13241-57). Two different methods were followed for rounds two and three, method 1 using the dimeric TGFbetaRII/Fc form of the antigen and method two using the soluble, monomeric form of TGFbetaRII. Method 1: Round two was performed with 1 nM biotinylated human TGFbetaRII/Fc with 1 uM non-biotinylated human TGFbetaRII/Fc competitor. Round three was performed with 100 pM biotinylated human TGFbetaRII/Fc with 1 uM non-biotinylated human TGFbetaRII/Fc (N12717-4). Method 2: Round two was performed with 1 nM biotinylated human TGFbetaRII with 1 uM non-biotinylated human TGFbetaRII competitor. Round three was performed with 100 pM biotinylated human TGFbetaRII with 1 uM non-biotinylated human TGFbetaRII competitor.

Second and third round selection outputs were subcloned into the pDOM13 vector, as described above. Individual clones were picked and expressed in 96 well plates at 850 rpm, 37° C. for 24 hours, 90% humidity in 0.5 ml/well overnight express auto-induction medium supplemented with 100 μg/ml carbenicillin. Plates were then centrifuged at 1800 g for 10 minutes. Supernatants were diluted either 1/5 or 1/2 in HBS-EP buffer and screened on BIACORE™ for binding to biotinylated human TGF-β RII/Fc (SA chip coated with 1000 Ru biotinylated hRII-Fc in accordance with the manufacturer's recommendations) (BIACORE™, GE HEALTHCARE™). Samples were run on BIACORE™ at a flow rate of 50 μl/min. Clones that bound with a high number of resonance units (RUs) or with an improved off-rate compared to the parent clone were expressed in 50 ml overnight express autoinduction medium at 30° C. for 48 to 72 hours and centrifuged at 4,600 rpm for 30 minutes. The supernatants were incubated overnight at 4° C. with Streamline-protein A beads. The beads were then packed into drip columns, washed with 5 column volumes of 2×PBS, followed by one bed volume of 10 mM Tris-HCl pH 7.4 and bound dAbs were eluted in 0.1 M glycine-HCl, pH 2.0 and neutralised with 1 M Tris-HCl, pH 8.0. The OD at 280 nm of the dAbs was measured and protein concentrations were determined using extinction coefficients calculated from the amino acid compositions of the dAbs.

In vitro analysis of off rate improved error prone selections: Purified dAbs were subjected to the same tests as those from the naive selections, namely, Biacore, SBE-bla HEK 293T Cell Sensor assay (h2), DSC, and SEC-MALS. Examples of clones improved over parent are shown in table 6A. IC50 values are a mean of ‘n’ number of experiments.

TABLE 6A On-rate Off-rate Affinity ka Fold kd Fold KD Fold Mean IC50 DOM23h ka1 (1/Ms) kd1 (1/s) KD improvement improvement improvement (nM)* 439 2.08E+06 5.02E−02 2.42E−08    3570 (3) 439-20 4.43E+06 3.54E−03 7.99E−10 2.1 14.2 30.3     48 (10) 843 9.05E+05 3.43E−01 3.78E−07    1947 (3) 843-13 5.11E+06 2.11E−02 4.13E−09 5.6 16.2 91.7    540 (4) 855 3.35E+05 3.15E−01 9.41E−07 >25000 (3) 855-21 1.86E+06 3.36E−02 1.80E−08 5.6  9.4 52.3   18580 (6) *number of experiments for calculation of mean IC50s provided in parenthesis

Affinity Matured Sequences

DOM23h-855-21 nucleic acid sequence (SEQ ID NO: 203) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTC TCCTGTGCAGCCTCCGGATTCACCTTTGAGAATACGAGTATGGGTTGGGTCCGCCAGGCT CCAGGGAAGGGTCTAGAGTGGGTCTCACGTATTGATCCTAAGGGTAGTCATACATACTAC ACAGACTCCGTGAAGGGCCGGTTCACCATCTCCCGCGACAATTCCAAGAATACGCTGTAT CTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTGTGCGAAACAGCGT GAGTTGGGTAAGTCGTAGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-855-21 amino acid sequence (SEQ ID NO: 204) EVQLLESGGGLVQPGGSLRLSCAASGFTFENTSMGWVRQAPGKGLEWVSRIDPKGSHTYY TDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKQRELGKSYFDYWGQGTLVTVSS DOM23h-843-13 nucleic acid sequence (SEQ ID NO: 205) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCCTGGTACAGCCTGGGGGGTCCCTGCGTCTC TCCTGTGCAGCCTCCGGATTCACCTTTGATCAGGATCGGATGTGGTGGGTCCGCCAGGCC CCAGGGAAGGGTCTAGAGTGGGTCTCAGCTATTGAGAGTGGTGGTCATAGGACATACTAC GCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCGCGACAATTCCAAGAACACGCTGTAT CTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTGTGCGAATCAGAAT AAGTCGGGGCGTTCGGGGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-843-13 amino acid sequence (SEQ ID NO: 206) EVQLLESGGGLVQPGGSLRLSCAASGFTFDQDRMWWVRQAPGKGLEWVSAIESGGHRTY YADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCANQNKSGRSGFDYWGQGTLVTVSS DOM23h-439-20 nucleic acid sequence (SEQ ID NO: 207) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTG TCTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCATGCGGCTGGGGTTTCGGGTACTTATTTTGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGC DOM23h-439-20 amino acid sequence (SEQ ID NO: 208) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFVSRIDSPGGRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKRHAAGVSGTYFDYWGQGTLVTVSS

Example 6 Affinity Maturation of DOM23h-271-7 Lineage

DOM23h-271 amino acid sequence (SEQ ID NO: 199) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQIPGRKWTANSRFDYWGQGTLVTVSS DOM23h-271 nucleic acid sequence (SEQ ID NO: 200) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATTCCGGGGCGTAAGTGGACTGCTAATTCGCGGTTTGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM23h-271-7 amino acid sequence (SEQ ID NO: 201) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQIPGRKWTANSRFDYWGQGTLVTVSS DOM23h-271-7 nucleic acid sequence  (SEQ ID NO: 202) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATTCCGGGGCGTAAGTGGACTGCTAATTCGCGGTTTGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC

Domain antibody DOM23h-271(SEQ ID No:199) had been isolated from the phage libraries in a previous selection campaign and variant DOM23h-271-7 (SEQ ID NO:201) was isolated following error prone affinity maturation, both as described in WO 2011/012609DOM23h-271-7 (SEQ ID NO:201) was selected for further affinity maturation based on its binding kinetics, sequence and biophysical behaviour. Affinity maturation was performed using degenerative mutagenesis to re-diversify the CDRs, and improved leads were identified using DNA display or phage display. Two types of libraries were constructed to re-diversify the CDRs, and these are referred to as triplet and doped libraries. To make the triplet libraries oligonucleotide primers were designed to cover each CDR, and within each primer the codons for three amino acids were replaced with NNS codons, so that three positions were diversified. Multiple oligonucleotides were used to cover all targeted amino acids within each CDR: 2 for CDR1, 3 for CDR2 and 6 for CDR3. Complementary oligonucleotide primers were used to amplify a sequence fragment containing each mutated CDR, and also an overlapping sequence fragment covering the rest of the dAb coding sequence. These fragments were mixed and assembled by splice extension overlap PCR to produce the full length dAb coding sequence. This product was PCR amplified using primers D0M172 (SEQ ID NO:187) and D0M173 (SEQ ID NO:188), digested with SalI and NotI, and ligated into similarly cut pDOM4 (described above) for phage selections, or pIE2A2 (described in WO2006018650) for DNA Display selections. The doped libraries were constructed using a similar method, essentially as described in WO2006018650. A single degenerate oligonucleotide primer was used to cover all mutations within each CDR. Within each primer the amino acids to be diversified were specified using degenerate codons to specify multiple amino acids. Five amino acids were diversified in CDR1, 7 in CDR2, and 13 in CDR3. In the primers the following degenerate coding is used: ‘a’=91% A+3% T+3% G+3% C; ‘g’=91% G+3% T+3% C.+3% A; ‘c’=91% C+3% T+3% G+3% A; ‘t’=91% T+3% A+3% G+3% C; ‘S’=50% G and 50% C. Capital letters indicate 100% of the specified nucleotide. The primers used were:

271-7R1deg CDR1 (SEQ ID NO: 189) (GCAGCCTCCGGATTCACCTTTacSgaStatagSATGtgSTGGGTCCGCCAGGCTCCGGGG); 271-7R2deg CDR2 (SEQ ID NO: 190) (GGGTCTCGAGTGGGTCTCAgcSATTgaSccSatSggSaaScgSACATACTACGCAGACTCCGTG); 271-7R3deg CDR3: (SEQ ID NO: 191) (GCGGTATATTACTGTGCGAAAcaSatSccSggScgSaaStgSacSgcSaaStcScgSttSGACTACTGGGGTCAGGG).

The degenerate library primers were used in the same way as the triplet primers. Each diversified CDR was amplified separately and then combined with a parental sequence fragment using splice extension overlap. The fragments were subcloned to pDOM4 and pIE2A2 using SalI and NotI.

DNA Display

Selections were performed using in vitro compartmentalisation in emulsions and DNA display using the scArc DNA binding protein essentially as described in WO2006018650. Briefly, TGFbRII-FC antigen was biotinylated using a 5:1 molar ratio of Biotin and the EZ-LINK™ Sulpho-NHS-LC-Biotin kit (Thermo #21327). A DNA fragment containing the Arc operator sequences and expression cassette containing the diversified dAb library was PCR amplified from the pIE2A2 vector using flanking primers. The product was purified from an eGel (Invitrogen) and diluted to 1.7 or 0.85 nM in 1 mg/ml BSA. For selection of improved binders the doped and Triplet libraries were processed separately under slightly different conditions. The Triplet CDR libraries were combined to give pooled CDR 1, 2 or 3 libraries. Ten rounds of selection were used for each type of library. For both methods, after 2 selection cycles, the diversified CDR's were amplified and recombined by splice overlap extension PCR to produce a 4th library with mutations in all 3 CDRs.

For the doped libraries 5×10⁸ copies of DNA were mixed with 50 ul of EXPRESSWAY™ In vitro translation mix (Invitrogen). Each reaction contained 10.0 μl SLYD™ extract; 10.0 μl 2.5× reaction buffer; 12.5 μl 2× feed buffer; 1.0 μl Methionine (75 mM); 1.26 μl Amino Acid mix (50 mM); 15 μl H₂O; 0.5 μl T7 Polymerase; 0.25 μl anti-HA mAb 3F10 (Roche, cat. 1 867 423); and 1.5 μl Glutathione (100 mM) (Sigma). This was added to 800 μl of hydrophobic phase (4.5% SPAN™-80, (Fluka)+0.5% Triton X-100 (Sigma) in Light white mineral oil (Sigma)) in a 4 ml glass vial (CHROMACOL™ 4SV P837) and stirred at 2000 rpm for 4-5 minutes. The tubes were sealed and incubated for 3 hours at 30° C. All subsequent steps were done at room temperature. To extract the DNA-protein complexes 200 μl of C+ buffer (10 mM Tris, 0.1 M KCl, 0.05% TWEEN™-20, 5 mM MgCl₂, 1% BSA, pH 7.4) and 500 μl of Hexane was added to the vial, mixed and transferred to a microtube and centrifuged at 13000 g for 1 minute. The organic phase was removed and the aqueous phase re-extracted with 800 μl of Hexane 3-5 more times until the interface was almost clear. For the first 5 rounds of selections the biotinylated TGFbRII-FC was pre-bound to Streptavidin DYNAbeads™ (Invitrogen) and added to the extracted complexes to give an antigen concentration equivalent to 40, 40, 10, 5 and 5 nM antigen (rounds 1, 2, 3, 4 and 5 respectively). T1 beads were used for selections 1-3, and C1 for selections 4 and 5. After 30 minutes incubation the beads were washed 3-5 times with C+ buffer. The DNA complexes remaining bound to the beads were then recovered by PCR with flanking primers. Selection rounds 6-10 were referred to as ‘soluble’ selections, where the Biotinylated TGFbRII-FC was added directly to the complexes after extraction to give a concentration of 5, 5, 4, 5 and 5 nM (rounds 6, 7, 8, 9 and 10 respectively), and incubated for 30 minutes to allow binding to be established. Non-biotinylated TGFbRII-FC was then added as a competitor to 74, 750, 400, 250 and 250 nM (rounds 6, 7, 8, 9 and 10 respectively), and incubated for 15, 15, 30, 30 and 30 minutes (rounds 6, 7, 8, 9 and 10 respectively). In rounds 9 and 10 a double stranded oligonucleotide containing the ARC operator sequence was included at 50 nM in the C+ buffer used in the hexane extractions to reduce cross-reactions between any non-complexed DNA and excess protein released from the emulsions. After the competition period 10 μl of C1 Streptavidin DYNAbeads™ were added. After 10 minutes the beads were washed 5 times with Buffer C+ and the bound complexes recovered by PCR with flanking primers as previously described. The PCR product was purified on an eGel and used for the next selection cycle. Following the 10th selection the recovered product was cut with SalI and NotI enzymes, and cloned into similarly cut pDOM13 for expression.

The triplet libraries were selected using a similar method, except that 1×10⁹ DNA copies were used in the first round selection, and 5×10⁸ thereafter. Also, the incubation time for protein expression in the emulsion was reduced to 2 hours. Soluble Biotinylated TGFbRII FC was used in all ten selection rounds at 25, 10, 5, 5, 5, 5, 2.5, 2.5, 2.5, 2.5, 2.5, 2.5 nM respectively. The Biotinylated target was incubated with the extracted complexes for 30 minutes. In selection rounds 5-10 the non-biotinylated TGFbRII-FC competitor was added to a final concentration of 250 nM for 15, 30, 60, 60, 75, and 90 minutes respectively before addition of C1 streptavidin DYNAbeads™. In round 5 competition was at room temperature, but from round 6 the competition temperature was increased to 30° C. The Arc Operator decoy oligo was included in selection rounds 1-4 to reduce cross complexing of defective DNA.

Following selections the dAb encoding inserts were excised from the DNA display expression cassettes using SalI and NotI, and cloned into the pDOM13 bacterial expression vector. The dAbs were sequenced and expressed in TB ONEX™ medium and supernatants were screened by BIACORE™ to identify clones with improved off-rates when compared to parent. Clones with improved off-rates were expressed and purified, and were assessed for affinity by BIACORE™ and potency in the cell sensor assay. Clones giving poor kinetic profiles, containing unfavourable sequence motifs, or giving very low yields were not pursued. Three were selected to be of further interest. Clones DOM23h-271-21 (SEQ ID NO:29) and DOM23h-271-22 (SEQ ID NO:30) were isolated from doped library selections. Clone DOM23h-271-27 (SEQ ID NO:31) was isolated from a triplet library selection. The affinity of the selected clones for human TGFbRII-FC is shown in table 7.

TABLE 7 Ka(M⁻¹ · s⁻¹) Kd (s⁻¹) KD (nM) DOM23h-271-7* 5.37E+6 5.10E−2 9.49 DOM23h-271-21 3.21E+6 4.72E−4 0.147 DOM23h-271-22 3.22E+6 9.19E−4 0.286 DOM23h-271-27 2.17E+6 1.26E−3 0.578 *N.B. values in the above table are for ranking purposes only since the fitting for DOM23h-271-7 to the 1:1 model was poor, although the affinity matured samples fitted well to this model.

Phage Display:

Triplet or doped libraries in separate CDR1, CDR2 and CDR3 pools were subjected to rounds of phage selection as described above against either biotinylated human TGF-β RII/Fc antigen over 4 rounds in concentrations of 10 nM, 1 nM, 100 pM and 20 pM respectively, or two rounds of selection using 20 pM followed by 2 pM antigen. Inserts from phage selections were cloned into the pDOM10 expression vector and supernatants with off rates improved over parent were selected for further study. Domain antibodies were expressed and purified their affinity and bioactivity against human TGF-β RII/Fc antigen tested on the BIACORE™ T100 and in the Cell sensor assay described above (data not shown). The affinity of the selected clones for human TGFbRII-FC is shown in table 8.

TABLE 8 Sample Ka (M−1 · s−1) Kd (s−1) KD (M) 271-101 3.73E+06 0.02014 5.40E−09 271-102 9.35E+06 0.01531 1.64E−09 271-105a* 3.29E+06 0.00747 2.27E−09 271-105b* 3.07E+06 0.007977 2.60E−09 271-106a* 7.39E+06 0.02333 3.16E−09 271-106b* 6.77E+06 0.01947 2.88E−09 271-114 1.04E+07 0.07084 6.80E−09 271-7a* 3.04E+06 0.04193 1.38E−08 271-7b* 2.42E+06 0.04448 1.84E−08 *The designation “a” and “b” refer to separate supernatants resulting from different colonies of the numbered clones.

The sequence of the selected clones with improved activity was determined and the full sequences and CDR sequences are shown below.

DOM23h-271-21 amino acid sequence  (SEQ ID NO: 29) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQMPGRKWTAKFRWDYWGQGTLVIVSS DOM23h-271-21 nucleic acid sequence  (SEQ ID NO: 67) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACCGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATGCCGGGCCGGAAGTGGACGGCCAAGTTCCGCTGGGACTACTGGGGTCAGGGAACCCTG GTCATCGTCTCGAGC DOM23h-271-22 amino acid sequence  (SEQ ID NO: 30) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQMPGQKWMAKSRFDYWGQGTLVTVSS DOM23h-271-22 nucleic acid sequence  (SEQ ID NO: 68) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATGCCCGGCCAGAAGTGGATGGCCAAGTCCCGCTTCGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM23h-271-27 amino acid sequence  (SEQ ID NO: 31) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGQKMADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQIPGRKWTANSRFDYWGQGTLVIVSS DOM23h-271-27 nucleic acid sequence  (SEQ ID NO: 69) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCAGAAGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATTCCGGGGCGTAAGTGGACTGCTAATTCGCGGTTTGACTACTGGGGTCAGGGAACCCTG GTCATCGTCTCGAGC DOM23h-271-101 amino acid sequence  (SEQ ID NO: 32) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQIPGRKWTANGRKDYWGQGTLVTVSS DOM23h-271-101 nucleic acid sequence  (SEQ ID NO: 70) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATTCCGGGGCGTAAGTGGACTGCTAATGGTCGTAAGGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM23h-271-102 amino acid sequence  (SEQ ID NO: 33) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQIPGRKWTANSRFDYWGQGTLVTVSS DOM23h-271-102 nucleic acid sequence  (SEQ ID NO: 71) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATTCCGGGGCGTAAGTGGACTGCTAATTCGCGGTTTGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM23h-271-105 amino acid sequence  (SEQ ID NO: 34) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTY YADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKQIPGQRWTGNSRFDYWGQGTL VTVSS DOM23h-271-105 nucleic acid sequence  (SEQ ID NO: 72) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATTCCGGGGCAGCGGTGGACTGGTAATTCGCGGTTTGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM23h-271-106 amino acid sequence  (SEQ ID NO: 35) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQFPGRKWTANSRSDYWGQGTLVTVSS DOM23h-271-106 nucleic acid sequence  (SEQ ID NO: 73) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGTTTCCGGGGCGTAAGTGGACTGCTAATTCGCGGTCTGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM23h-271-114 amino acid sequence  (SEQ ID NO: 36) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQIPGRKGTANSRFDYWGQGTLVTVSS DOM23h-271-114 nucleic acid sequence  (SEQ ID NO: 74) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTAATCGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGATTCCGGGGCGTAAGGGAACTGCTAATTCGCGGTTTGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC

TABLE 9 CDR sequences of 271 affinity matured clones with improved activity CDR1 (Kabat 26-35) CDR2 (Kabat 50-65) CDR3 (Kabat 95-102) DOM23h- GFTFTEYRMW AIEPIGNRTYYADSVKG QMPGRKWTAKFRWDY 271-21 (SEQ ID NO: 105) (SEQ ID NO: 141) (SEQ ID NO: 177) DOM23h- GFTFTEYRMW AIEPIGNRTYYADSVKG QMPGQKWMAKSRFDY 271-22 (SEQ ID NO: 106) (SEQ ID NO: 142) (SEQ ID NO: 178) DOM23h- GFTFTEYRMW AIEPIGQKTYYADSVKG QIPGRKWTANSRFDY 271-27 (SEQ ID NO: 107) (SEQ ID NO: 143) (SEQ ID NO: 179) DOM23h- GFTFTEYRMW AIEPIGNRTYYADSVKG QIPGRKWTANGRKDY 271-101 (SEQ ID NO: 108) (SEQ ID NO: 144) (SEQ ID NO: 180) DOM23h- GSTFTEYRMW AIEPIGHRTYYADSVKG QIPGRKWTANSRFDY 271-102 (SEQ ID NO: 109) (SEQ ID NO: 145) (SEQ ID NO: 181) DOM23h- GFTFTEYRMW AIEPIGNRTYYADSVKG QIPGQRWTGNSRFDY 271-105 (SEQ ID NO: 110) (SEQ ID NO: 146) (SEQ ID NO: 182) DOM23h- GFTFTEYRMW AIEPIGNRTYYADSVKG QFPGRKWTANSRSDY 271-106 (SEQ ID NO: 111) (SEQ ID NO: 147) (SEQ ID NO: 183) DOM23h- GFTFTEYRMW AIEPIGNRTYYADSVKG QIPGRKGTANSRFDY 271-114 (SEQ ID NO: 112) (SEQ ID NO: 148) (SEQ ID NO: 184) N.B CDR2 and CDR3 are as defined by Kabat. CDR1 is defined by a combination of the Kabat and Chothia methods.

Generic Method for Binding Kinetics—T100

BIACORE™ analysis was carried out using a capture surface on a CM4 chip. Anti-human IgG was used as the capturing agent and coupled to a CM4 biosensor chip by primary amine coupling. The Antigen molecule fused to the human Fc was captured on this immobilised surface to a level from 250 to 300 resonance units and defined concentrations of Domain antibodies diluted in running buffer were passed over this captured surface. An injection of buffer over the captured antigen surface was used for double referencing. The captured surface was regenerated, after each domain antibody injection using 3M magnesium chloride solution; the regeneration removed the captured antigen but did not significantly affect the ability of the surface to capture antigen in a subsequent cycle. All runs were carried out at 25° C. using HBS-EP buffer as running buffer. Data were generated using the BIACORE™ T100 and fitted to the 1:1 binding model inherent to the software. When non-specific binding was seen at the top concentration, the binding curve at this concentration was removed from the analysis set.

Further Diversification of CDR3

The DOM23h-271-7 derivatives with the highest affinity contained methionines in position 96 and 100B. These positions, along with positions 99, 100D, 100E and 100G were diversified using NNK mutagenesis to determine whether substitutions could be made. The NNK library was constructed as described above using primer PEP-26-F to introduce diversity at the selected positions in DOM23h-271-22 or DOM23h-271-102 background. DOM23h-271-102 contains mutations at position 27 and 55 that confer improved affinity over DOM23h-271-7.

PEP-26-F  (SEQ ID NO: 209) GCGGTATATTACTGTGCGAAACAGNNSCCCGGCNNSAAGTGGNNSGCCNNSNNSCGCNNSGACTACTGGGG TCAGGGAACC

DNA display libraries were constructed and selected on biotinylated hTGFbRII-FC as described above using concentrations of 5 nM; 0.5 nM; 0.1 nM; 0.1 nM; 0.1 nM; and 0.1 nM in successive rounds. In selection rounds 4-6 the non-biotinylated TGFbRII-FC competitor was added to a final concentration of 100 nM for 60, 90, and 90 minutes respectively before addition of C1 streptavidin DYNAbeads™. Following selection the dAb inserts were PCR amplified using primers PeIB NcoVh and PEP011, cut with NcoI and EcoRI, and cloned into the bacterial expression vector pC10.

PelB NcoVh (SEQ ID NO: 210) GCCCAGCCGGCCATGGCGGAGGTGCAGCTGTTGGAGTCTGGG PEP011  (SEQ ID NO: 211) GAATTCGCGGCCGCCTATTAGCTCGAGACGGTGACCAGGG

The cloned products were expressed and screened by Biacore. Clones with off-rates similar or better than DOM23h-271-22 were sequenced, purified, and assessed for affinity by BIACORE™ and potency in the cell sensor assay. Clones giving poor kinetic profiles, containing unfavourable sequence motifs, or giving very low yields were not pursued. DOM23h-271-50 was selected for further affinity maturation.

DOM-271-50 nucleic acid sequence  (SEQ ID NO: 212) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCGGCGAGAAGTGGCTCGCCCGGGGCCGCTTGGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGC DOM-271-50 amino acid sequence (SEQ ID NO: 213 and duplicate entry SEQ ID NO: 214) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPGEKWLARGRLDYWGQGTLVTVSS

Example 7 Introduction of Mutations into TGFbRII dAb Sequences (DOM23h-271 Lineage) at Positions 61 and 64

The D61N and K64R double mutations have previously been introduced into various TGFRβII dAb lineages and have been shown to improve potency (WO2011/012609). These mutations were introduced into TGFbRII dAb DOM23h-271 lineages to see if a similar improvement in potency could be achieved. Alternative mutations at this position were explored to determine whether further enhancements in potency could be achieved.

Mutations were introduced into the DOM23h-271 (SEQ ID NO:199) backbone by overlap extension using the polymerase chain reaction (PCR) (Ho, et al., Gene 1989 77(1)); complementary oligonucleotide primers were used to generate two DNA fragments having overlapping or complementary ends. These fragments were combined in a subsequent assembly PCR in which the overlapping ends anneal, allowing the 3′ overlap of each strand to serve as a primer for the 3′ extension of the complementary strand and the resulting fusion product to be amplified further by PCR. Specific alterations in the nucleotide sequence were introduced by incorporating nucleotide changes into the overlapping oligonucleotide primers. The target dAb gene fragments were amplified by two separate PCRs using SUPERTAQ™ polymerase (HT Biotechnology). DOM23h-271 was selected as it has a low affinity for TGFbRII so that improvements in affinity were clearly measurable using BIACORE™. The mutations at positions 61 and 64 were encoded using a degenerate oligo-nucleotide to mutate both positions in combination, with the intension of using affinity selection to enrich for mutants with improved binding affinity. As the NR mutation is known to dominate this mutation was avoided in the library by employing a restricted codon, NNG, at position 61. This codon encodes 13 amino acids but does not include the amino acids F, Y, C, H, I, N or D. Free Cys was not favoured within a dAb, and asparagine was also not wanted at this position, so only 5 desirable amino acids combinations were excluded. At position 64 an NNK codon was used to provide the full range of possible amino acids.

The oligonucleotide pairs used to introduce the mutations were PE008 (flanks the 5′ start of the dAb gene: 5′-TTGCAGGCGTGGCAACAGCGTCGACAGAGGTGCAGCTGTTGGAG-3′) (SEQ ID NO:192) with 271-6164 R (5′-GCGTAGTATGTACGATTACCAATCGG-3′) (SEQ ID NO:193) and mutated 271-6164 deg-F (mutated residues underlined: 5f-GGTAATCGTACATACTACGCANNGTCCGTGNNKGGCCGGTTCACCATCTCCCGC-3′) (SEQ ID NO: 194) with AS1309 (flanks the 3′ end of the dAb gene: 5′-TGTGTGTGTGTGGCGGCCGCGCTCGAGACGGTGACCAGGGTTCCCTGACCCCA-3′) (SEQ ID NO:195). The two PCR fragments were recombined in an assembly PCR using SUPERTAQ™ DNA polymerase without the addition of primers. The fusion product was then amplified by the addition of flanking primers PE008 and AS1309 to the PCR reaction. The mutated dAb was digested with Sal I and Not I restriction endonucleases, ligated into similarly cut pDOM13 expression vector and transformed into E. Coli HB2151 cells. The D61N, K64R mutation was also introduced into DOM23h-271 in the same way, but substituting primer 271-6164 NR—F (5′-GGTAATCGTACATACTACGCAAACTCCGTGCGCGGCCGGTTCACCATCTCCCGC-3′) (SEQ ID NO:196) for 271-6164 deg-F. The mutated dAbs were identified by sequencing. 129 clones with novel combinations of mutations at position 61 and 64 were identified, though 11 carried additional mutations from PCR errors. These are shown in table 11. N.B. Clones with enhanced affinity are underlined. Clones with additional mutations are indicated by asterisks

To determine the effect of the mutations the selected clones were expressed in TB ONEX™ in 96 well plates for 72 hours at 30° C. or 24 hours at 37° C. The supernatants were clarified by centrifugation and filtered through a 0.22 μm filter before off-rate evaluation by BIACORE™

BIACORE™ A100 analysis was carried out using a capture surface on a CM5 chip. Anti-human IgG was used as the capturing agent and coupled to a CM5 biosensor chip by primary amine coupling. The Antigen molecule fused to the human Fc was captured on this immobilised surface to a level from 200 to 300 resonance units and supernatants or purified domain antibodies were passed over this captured surface. An injection of media or buffer over the captured antigen surface was used for double referencing. On each flow cell, a protein A spot allowed to confirm the presence of domain antibody on each sample injected. The surfaces were regenerated, after each domain antibody injection using 3M magnesium chloride solution; the regeneration removed the captured antigen but did not significantly affect the ability of the surface to capture antigen in a subsequent cycle. All runs were carried out at 25° C. using HBS-EP buffer as running buffer. Data were generated using the BIACORE™ A100 and analyzed using its inherent software. Kinetics from purified samples were fitted to the 1:1 binding model while supernatant off-rates were analyzed using the 1:1 dissociation model and/or using the binding level and the stability level of each sample in comparison to the parent molecule.

Of clones tested 46 were identified with improved off-rate by comparison to the parent DOM23h-271 dAb, as indicated in table 11. Mutations D61R and D61K were found to enhance binding independent of mutations at position 64. A number of combinations appeared better than the original D61N, K64R mutations, and these included RE, RM, RF and RY.

A selection of mutants with improved off-rate were purified and subjected to full BIACORE™ A100 kinetic analysis to determine their affinity (Table 10). The thermal stability of the mutants was also determined by generation of melting profiles in the presence of SYPRO™ Orange (Invitrogen). Briefly, the purified dAbs were diluted to 50 and 100 ug/ml in a 1:500 dilution of SYPRO™ Orange in PBS, and subjected to a 30-90° C. melt curve in a Mini OPTICON™ PCR machine (BioRad). The Tm of the major positive transition was determined at each concentration and used to calculate an estimated Tm value at 1 mg/ml as an indication of the melting temperature for comparison (Table 10).

The mutations D61R, K64D and D61R, K64F were selected as they had a good improvement in affinity for a reduced impact in Tm. These mutations were transferred to an affinity matured DOM23h-271 derivative DOM23h-271-22 (SEQ ID NO:30) to make dAbs DOM23h-271-39 (SEQ ID NO:37) and DOM23h-271-40 (SEQ ID NO:38). These mutations were found to give an enhancement in affinity by BIACORE™ including cross reactivity with murine TGFbRII FC, and enhanced potency by cell sensor assay. However the mutations were also found to reduce the Tm as measured by DSC, and increase aggregation of the dabs in PBS, as measured by SEC MALLS. These assays were carried out as described above, except that the buffer for SEC MALLS was 0.4M Nacl, 0.1M Sodium Phosphate and 10% isopropanol pH7. These results are summarized in table 12 (mean values are given for the human cell sensor assay and the mouse 3T3 luciferase assay).

Sequences of dabs referred to in this example are given below:

DOM23h-271-39 amino acid sequence (SEQ ID NO: 37) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYARSVDGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQMPGQKWMAKSRFDYWGQGTLVTVSS DOM23h-271-39 nucleic acid sequence  (SEQ ID NO: 75) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGCCTCC GGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGGTCTCAGCG ATTGAGCCGATTGGTAATCGTACATACTACGCACGCTCCGTGGACGGCCGGTTCACCATCTCCCGCGACAATTCCA AGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACTGTGCGAAACAGATGCC CGGCCAGAAGTGGATGGCCAAGTCCCGCTTCGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC DOM23h-271-40 amino acid sequence  (SEQ ID NO: 38) EVQLLESGGGLVQPGGSLRLSCAASGFTFTEYRMWWVRQAPGKGLEWVSAIEPIGNRTYYARSVFGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQMPGQKWMAKSRFDYWGQGTLVIVSS DOM23h-271-40 nucleic acid sequence  (SEQ ID NO: 76) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGCCTCC GGATTCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGGTCTCAGCG ATTGAGCCGATTGGTAATCGTACATACTACGCACGCTCCGTGTTCGGCCGGTTCACCATCTCCCGCGACAATTCCA AGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACTGTGCGAAACAGATGCC CGGCCAGAAGTGGATGGCCAAGTCCCGCTTCGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCGAGC

TABLE 10 Description Mutation Est. Tm 1 mg/ml KD (nM) 1A2 RA 53.08 2.24 1D9 RD 52.35 1.17 3F11 RE 53.08 3.27 1G11 RM 51.81 0.97 3C9 RF 54.08 1.39 1E6 RY 49.62 1.17 1B11 RV 53.41 1.88 3D9 KG 48.69 4.34 1D5 KF 48.09 3.69 3D4 KT 54.74 4.26 1H11 LW 53.68 4.17 1B2 VW 52.68 1.79 2C12 NR 51.49 3.34 271 DK 58.21 N/D N.B. the mutation corresponds to ‘XY’ wherein X is position 61 and Y is position 64

TABLE 11 Mutations inserted into DOM23h-271 at positions 61 and 64. 61\64 A R N D C Q E G H I L K M A 1D6* 1C10 3B11 R 1A2 1A3 3F4 1D9 2C4 1A8 3F11 3G8 1A4 3C2 1G11 N 2C12 D 3H6 (wt) C Q 1B6* 1E8 1B10 1H12 1D11 E 2A3 1F12 2G4 3H7 G 1D1 1B5 3B12 1E5 3G11 3D12 H I L 1D2 3E11 2A9 1B4 1H3 1A9 1F1 1G9 1H10 1C9 K 2E5 — 2B4 3D9 3E12 M 1G6 2D3* 1E7 2E6 3C12 F P 2A8 1A5 2C11 S 3C3 3E7 3E5 1H4 2B10 1C11 T 1E3 1D10* 2C1 1C1 2A5 3G9 W 2F10 1E12 2C2 2A2 2A6 1D4 Y V 1C12 2B9 1H6 1D3* 3C1 2G2 2F7 1F7 61\64 F P S T W Y V A 3C10* 3B10 1A12 3G3* R 3C9* 2F5 1H1 1H2 1E6 1B11 N D C Q 1C8 3F7 2C10 E 1F10 2B11 3D6 G 2B6 2G5 2D5 H I L 2C9 1H5 1G2 1E4 1H11 K 1D5 1G7 2E9* 3D4 1B1 1F2 M 2F6 3H1 3B5* 1A6 1E11 3B8* F P 3H2 1G5 3G12 S 1B7 2C8 3B1 3D5 T 2H10 2A4 3D11 W 3B2 2B1 Y V 2H4 3C7 1B2 2G6 Clones with improved off-rate are underlined. Clones with additional mutations are indicated by asterisks

TABLE 12 Human Cell Mouse 3T3 Human Cyno Mouse sensor Luciferase assay TGFbRII TGFbRII TGRbRII Tm Aggregation Mutation IC50 (nM) IC50 (nM) KD (pM) KD (pM) KD (pM) (DSC) (SEC-MALS) DOM23h-271- — 39.01 >17780 202.00 225.00 — 60 91.8% Monomer 22  8.2% Dimer DOM23h-271- RD  1.17   11120  14.90  24.00 1780.00 57.5 51.6% M/D Eq 39 38.5% D/T Eq + aggregates DOM23h-271- RF  0.53    1200  9.99  11.25  548.50 55.6 91.6% M/D Eq 40  8.4% Trimer/ oligomer

Example 8 Affinity Maturation by CDR Diversification of Lineages DOM23h-439-20, DOM23h-843-13, DOM23h-855-21 and DOM23h-271-50

Domain antibodies DOM23h-855-21(SEQ ID NO:204) and DOM23h-843-13 (SEQ ID NO:206) were isolated from the error prone affinity maturation carried out on naive clones DOM23h-855 (SEQ ID NO:13) and DOM23h-843 (SEQ ID NO:10) respectively, as detailed in example 5. Domain antibody DOM23h-439 was isolated from phage libraries in a previous selection campaign described in WO 2011/012609 and variant DOM23h-439-20 (SEQ ID NO:208) was subsequently isolated by error prone affinity maturation as detailed in example 5. Domain antibody DOM23h-271-50 (SEQ ID NO:214) was generated by CDR3 re-diversification of CDR-directed affinity matured variant DOM23h-271-22 (SEQ ID NO:30) as detailed in Example 6. DOM23h-855-21, DOM23h-843-13, DOM23h-439-20 and DOM23h-271-50 were all selected for further affinity maturation based on their binding kinetics, potency in the SBE-bla HEK 293T cell sensor assay, sequence and biophysical behaviour. Affinity maturation was performed using degenerative mutagenesis to re-diversify the CDRs, and improved leads were identified using phage display. Diversity was introduced into the CDRs by construction of either doped or NNK libraries.

DOM23h-271-50 was affinity matured using NNK libraries (saturation mutagenesis), oligonucleotide primers were designed to cover each CDR and within each primer the codons for 5 amino acids were replaced with NNK codons so that 5 positions are diversified simultaneously. Single or multiple oligonucleotides were used to cover all targeted amino acids within each CDR; 1 for CDR1, 2 for CDR2 and 3 for CDR3. CDR-directed affinity maturation was achieved using polymerase chain reaction (PCR); Complementary oligonucleotide primers were used to amplify a sequence fragment containing each mutated CDR, and also an overlapping sequence fragment covering the rest of the dAb coding sequence. These fragments were mixed and assembled by splice extension overlap PCR to produce the full length dAb coding sequence. This product was PCR amplified using primers PeIB NcoVh (SEQ ID NO:210) and PEP044 (5f-GGAACCCTGGTCACCGTCTCGAGCGCGGCCGCATAATAAGAATTCA-3′ SEQ ID NO:215), digested with NcoI and NotI, and ligated into NotI and NcoI digested pDOM4-gene3-pelB hybrid vector. pDOM4-gene3-pelB hybrid vector is a modified version of the pDOM4 vector described above but has been modified to replace the GAS leader sequence with the pelB (pectate lyase B) signal peptide.

The domain antibodies DOM23h-855-21, DOM23h-843-13 and DOM23h-439-20 were affinity matured using doped libraries. The doped libraries were constructed essentially as described above and in WO2006018650. A single degenerate oligonucleotide primer was used to cover all mutations within each CDR. Within each primer the amino acids to be diversified were specified using degenerate codons to encode multiple amino acids. The following degenerate coding was used: ‘a’=85% A+5% T+5% G+5% C; ‘g’=85% G+5% T+5% C+5% A; ‘c’=85% C+5% T+5% G+5% A; ‘t’=85% T+5% A+5% G+5% C.; ‘S’=50% G and 50% C. Capital letters indicate 100% of the specified nucleotide. For the DOM23h-439-20 doped library five amino acids were diversified in CDR1, 6 in CDR2 and 11 in CDR3 to include the phenyalanine at position 100. For the DOM23h-855-21 doped library five amino acids were diversified in CDR1, 7 in CDR2 and 8 in CDR3. For the DOM23h-843-13 doped library five amino acids were diversified in CDR1, 6 in CDR2 and 8 in CDR3, position 94 before the CDR3 was also diversified by introducing the codon VRK. For each of the dAbs, 4 doped libraries were constructed, one to diversify CDR1, one to diversify CDR2, one to diversify CDR3 and a fourth where all CDRs were diversified. The degenerate library primers were used in the same way as the triplet primers. Each diversified CDR was amplified separately and then combined with a parental sequence fragment using splice extension overlap, the full length product was amplified using primers PeIB NcoVh (SEQ ID NO:210) and D0M173 (SEQ ID NO:188). The fragments were subcloned to pDOM4-gene3-pelB hybrid vector using NcoI and NotI.

Degenerate Primer Sequences:

23h-439-20 CDRH1  (SEQ ID NO: 216) 5′-GCAGCCTCCGGATTCACCTTTggSacSgagcagATGtggTGGGTCCGCCAGGCTCCAGGG-3′ 23h-439-20 CDRH2  (SEQ ID NO: 217) 5′-AAGGGTCTAGAGTTTGTCTCAcgSATTgattcSccSGGTggScgSACATACTACGCAGACTCCGTG-3′ 23h-439-20 CDRH3  (SEQ ID NO: 218) 5′-GCGGTATATTACTGTGCGAAAcgScatgcSgcSggSgtStcSggSacStaYtttGACTACTGGGGTCAGGGAACC-3′ 23h-843-13 CDRH1  (SEQ ID NO: 219) 5′-GCAGCCTCCGGATTCACCTTTgatcaggatcgSATGtggTGGGTCCGCCAGGCCCCAGGG-3′ 23h-843-13 CDRH2  (SEQ ID NO: 220) 5′-AAGGGTCTAGAGTGGGTCTCAgcSATTgagtcSggSGGTcatcgSACATACTACGCAGACTCCGTG-3′ 23h-843-13 CDRH3  (SEQ ID NO: 221) 5′-ACCGCGGTATATTACTGTGCGVRKcagaataagtcSggScgStcSggSTTTGACTACTGGGGTCAGGGA-3′ 23h-855-21 CDRH1  (SEQ ID NO: 222) 5′-GCAGCCTCCGGATTCACCTTTgagaatacStcSATGggSTGGGTCCGCCAGGCTCCAGGG-3′ 23h-855-21 CDRH2  (SEQ ID NO: 223) 5′-AAGGGTCTAGAGTGGGTCTCAcgSATTgatccSaagGGTtcScatACATACTACacSGACTCCGTGAAGGGCCGGT TCACC-3′ 23h-855-21 CDRH3  (SEQ ID NO: 224) 5′-GCGGTATATTACTGTGCGAAAcagcgSgagctSggSaagtcStaYTTTGACTACTGGGGTCAGGGA-3′ H1-271-43 R  (SEQ ID NO: 225) 5′-GCAGCCTCCGGATTCACCTTTNNKNNKNNKNNKATGNNKTGGGTCCGCCAGGCTCCGGGGAAGGGTCTC-3′ H2p1-271-43F  (SEQ ID NO: 226) 5′-CCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGGTCTCANNKATTNNKNNKNNKGGTNNKCGTACATACTACG CAGACTCCG-3′ H2p2-271-43 F  (SEQ ID NO: 227) 5′-CCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGGTCTCAGCGATTGAGNNKNNKNNKNNKNNKACATACTACG CAGACTCCG-3′ H3p1-271-43 F  (SEQ ID NO: 228) 5′-ACCGCGGTATATTACTGTGCGAAANNKNNKNNKNNKNNKAAGTGGATGGCCGTGGGCCGCTTGGACTACTG GGGTCAGGG-3′ H3p2-271-43 F  (SEQ ID NO: 229) 5′-ACCGCGGTATATTACTGTGCGAAACAGAAGCCCNNKNNKNNKNNKNNKGCCGTGGGCCGCTTGGACTACTG GGGTCAGGG-3′ H3p3-271-43 F  (SEQ ID NO: 230) 5′-ACCGCGGTATATTACTGTGCGAAACAGAAGCCCGGCCAGAAGTGGNNKNNKNNKNNKNNKTTGGACTACTG GGGTCAGGG-3′

Phage Display:

NNK or doped libraries in separate CDR1, CDR2, CDR3 and combined CDR1, 2 and 3 pools were subjected to at least 6 rounds of selection against 100 nM, 10 nM, 1 nM, 1 nM, 0.1 nM and 0.1 nM (rounds 1, 2, 3, 4, 5 and 6 respectively) biotinylated monomeric human TGF-β RII antigen as described in example 1 with the following deviation to the block step; the human IgG Fc fragment which was previously added in example 1 was omitted and the block step performed for a minimum of 20 minutes. In rounds 4 and 6 of selection competition was introduced by incubation with 100 nM non-biotinylated antigen for 30 min (rounds 4 and 6) following the incubation step with the labelled antigen. For DOM 23h-439-20 (CDR1, CDR3 and combined pools) and DOM 23h-855-21 (CDR3) a seventh round of selection was carried out against 0.1 nM biotinylated monomeric human TGF-β RII antigen and 100 nM competition for 120 min or 20 pM biotinylated monomeric human TGF-β RII antigen with 100 nM competition for 30 min. Phage were amplified between rounds of selection by centrifugation of an overnight culture of phage infected TG1 cells for 30 minutes at 4000 g. 40 ml of supernatant containing the amplified phage was added to 10 ml of PEG/NaCl (20% v/w PEG 8000+2.5M NaCl) and incubated on ice for 60 minutes. The samples were centrifuged for 40 minutes at 4000 g to pellet the precipitated phage. The supernatant was discarded and the phage pellet was resuspended in 1 ml 15% v/v glycerol/PBS. The phage sample was transferred to 2 ml Eppendorf tubes and centrifuged for 10 minutes to remove any remaining bacterial cell debris. Diversified domain antibody Vh genes from the phage selections were PCR amplified using primers PEP011VHStopNotIR (5′-CCCTGGTCACCGTCTCGAGCTAATAGGCGGCCGCGAATTC-3′ (SEQ ID NO: 231) and Nco1 VH F (5′-TATCGTCCATGGCGGAGGTGCAGCTGTTGGAGTCTGG-3′ (SEQ ID NO: 232), digested with NcoI and NotI restriction endonucleases and ligated into the pC10 vector also digested with NcoI and NotI. pC10 is a plasmid vector based on pUC119 vector, with expression under the control an enhanced LacZ promoter designed for soluble expression of dAbs. Expression of dAbs into the supernatant is enabled by fusion of the dAb gene to the pelB signal peptide at the N-terminal end. The ligation products were transformed into chemically competent E. coli HB2151 cells and plated on nutrient agar plates supplemented with 100 μg/ml of carbenicillin. Individual clones were picked and expressed in overnight express auto-induction medium (high-level protein expression system, Novagen), supplemented with 100 μg/ml carbenicillin in 96-well plates and grown with shaking at 250 rpm for either 66 hours at 30° C. or 24 hours at 37° C. Expression plates were then centrifuged at 3500 g for 15 minutes and the supernatants filtered using 0.45μ filter plates (Millipore). Supernatants containing the domain antibodies were screened on the BIACORE™ B4000 against human TGF-β RII and human TGF-β RII/Fc to determine the off-rate (Kd) (data not shown). Biotinylated antigens were captured on an SA chip in accordance with the manufacturer's instructions, analysis was carried out at 25° C. using HBS-EP buffer. Samples were run on BIACORE™ at a flow rate of 30 μl/min. Regeneration of the chip was achieved using glycine at low pH. The data (not shown) were analysed for off-rate by fitting the dissociation phase to the 1:1 dissociation model inherent to the software, supernatants were also analyzed for protein A binding to estimate levels of expression. Domain antibodies with off rates improved over parent were selected for further study. Improved clones were expressed in overnight express autoinduction medium (ONEX™, Novagen) supplemented with 100 ug/ml carbenicillin and antifoam (Sigma) at 30° C. for 48 to 72 hours with shaking at 250 rpm. The cultures were centrifuged (4,200 rpm for 40 minutes) and the supernatants were incubated with STREAMLINE™-protein A beads (Amersham Biosciences, GE HEALTHCARE™, UK. Binding capacity: 5 mg of dAb per ml of beads), at 4° C. or at room temperature for at least one hour. The beads were packed into a chromatography column and washed with PBS, followed by 10 mM Tris-HCl pH 7.4 (Sigma, UK). Bound dAbs were eluted with 0.1 M glycine-HCl pH 2.0 and neutralized with 1M Tris-HCL pH 8.0. The OD at 280 nm of the dAbs was measured and protein concentrations were determined using extinction coefficients calculated from the amino acid compositions of the dAbs. Affinity matured domain antibodies were tested on the BIACORE™ T200 (data for two preferred dAbs are shown in Example 9) and in the SBE-bla HEK 293T Cell Sensor assay (data for two preferred dAbs are shown in Example 11) to determine their affinity and potency. Biophysical properties including thermal stability and solution state were determined using Differential Scanning Colourimetry (DSC) and size exclusion chromatography with multi-angle-LASER-light scattering (SEC-MALS) (data for two preferred dAbs are shown in Example 10).

The amino acid and nucleic acid sequences of affinity matured DOM23h-439-20 and DOM23h-271-50 anti-human TGFRII dAbs

DOM23h-439-25 nucleic acid sequence  (SEQ ID NO: 233) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTG TCTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGC DOM23h-439-25 amino acid sequence  (SEQ ID NO: 234) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFVSRIDSPGGRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSS DOM23h-271-123 nucleic acid sequence  (SEQ ID NO: 235) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCGGCGAGAAGTGGGCGAGGCGGTGGGATTTGGACTACTGGGGTCAGGGAACCCT GGTCACCGTCTCGAGC DOM23h-271-123 amino acid sequence  (SEQ ID NO: 236) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPGEKWARRWDLDYWGQGTLVTVSS DOM23h-439-35 nucleic acid sequence  (SEQ ID NO: 237) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTC TCCTGTGCAGCCTCCGGATTCACCTTTGGGACCGATCAGATGTGGTGGGTCCGCCAGGCT CCAGGGAAGGGTCTAGAGTTTGTCTCACGCATTGATTCCCCCGGTGGGCGGACATACTAC GCAAACTCCGTGAAGGGCCGGTTCACCATCTCCCGCGACAATTCCAAGAACACGCTGTAT CTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACTGTGCGAAACGGCAG CCGGCGGGGGTGTCGGGGAAGTACGTTGACTACTGGGGTCAGGGAACCCTGGTCACCGTC TCGAGC DOM23h-439-35 amino acid sequence  (SEQ ID NO: 238) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTDQMWWVRQAPGKGLEFVSRIDSPGGRTYYANSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKRQPAGVSGKYVDYWGQGTLVTVSS DOM23h-271-129 nucleic acid sequence  (SEQ ID NO: 239) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTC TCCTGTGCAGCCTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCT CCGGGGAAGGGTCTCGAGTGGGTCTCAGCGATTGAGCCGATTGGTCATAGGACATACTAC GCAGACTCCGTGAAGGGCCGGTTCACCATCTCCCGCGACAATTCCAAGAACACGCTGTAT CTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACTGTGCGAAACAGGCG CCCAATCAGAGGTATGTTGCCCGGGGCCGCTTGGACTACTGGGGTCAGGGAACCCTGGTC ACCGTCTCGAGC DOM23h-271-129 amino acid sequence  (SEQ ID NO: 240) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPNQRYVARGRLDYWGQGTLVIVSS

The CDRs as defined by Kabat of these anti-human TGFRII dAb affinity leads are shown in Table 13

TABLE 13 CDR Sequences of affinity matured anti-human TGFβRII dAbs CDR1 (Kabat 26-35) CDR2 (Kabat 50-65) CDR3 (Kabat 95-102) DOM23h- GSTFTEYRMW AIEPIGHRTYYADSVKG QAPGEKWARRWDLDY 271423 (SEQ ID NO: 241) (SEQ ID NO: 242) (SEQ ID NO: 243) DOM23h- GSTFTEYRMW AIEPIGHRTYYADSVKG QAPNQRYVARGRLDY 271429 (SEQ ID NO: 244) (SEQ ID NO: 245) (SEQ ID NO: 246) DOM23h- GFTFGTEQMW RIDSPGGRTYYADSVKG RRPTGVSGTFYDY 439-25 (SEQ ID NO: 247) (SEQ ID NO: 248) (SEQ ID NO: 249) DOM23h- GFTFGTDQMW RIDSPGGRTYYANSVKG RQPAGVSGKYVDY 439-35 (SEQ ID NO: 250) (SEQ ID NO: 251) (SEQ ID NO: 252) Further Modification of the DOM23h-439-25 and DOM23h-271-123 Nucleotide Sequence

The D61N and K64R mutations as described in example 7 were introduced into the DOM23h-439-25, DOM23h-271-123 and DOM23h-271-129 affinity matured dAbs either in combination or separately. Introduction of a V48I mutation in the DOM23h-439-25 and DOM23h-271-123 dAbs was also tested to determine whether it could confer improvements in potency. Spontaneous mutation at kabat position 48 was observed in a number of improved dAbs from the DOM23h-439 lineage following both test maturation and CDR-directed affinity maturation. An Alanine at the C-terminus of the Vh region, immediately after kabat residue 113 was also added to DOM23h-439-25, DOM23h-271-123 and DOM23h-271-129 and all variants of these dAbs with the afore-mentioned mutations. Mutations were introduced into the DOM23h-439-25 (SEQ ID NO: 234), DOM23h-271-123 (SEQ ID NO: 236) and DOM23h-271-129 (SEQ ID NO: 240) backbones by overlap extension using the polymerase chain reaction (PCR) essentially as described in Example 7. Specific mutations in the nucleotide sequence were introduced by incorporating nucleotide changes into the overlapping oligonucleotide primers, the insertion of the Alanine at the end of the Vh region was achieved by using a 3′ oligonucleotide designed to incorporate the Alanine residue after kabat position 113.

The following oligonucleotides were used to introduce the mutations (mutated residues underlined):

439 48I SDM F  (SEQ ID NO: 253) 5′-GGGTCTAGAGTTTATTTCACGTATTGATTCGCC-3′ 439 61N SDM F  (SEQ ID NO: 254) 5′-GGGAGGACATACTACGCAAACTCCGTGAAGGGCCGG-3′ 439 64R SDM F  (SEQ ID NO: 255) 5′-CGCAGACTCCGTGCGTGGCCGGTTCACC-3′ 439 61N 64R SDM F  (SEQ ID NO: 256) 5′-GGGAGGACATACTACGCAAACTCCGTGCGTGGCCGGTTCACC-3′ 271 61N SDM F  (SEQ ID NO: 257) 5′-GGACATACTACGCAAACTCCGTGAAGGGCCGG-3′ 271 64R SDM F  (SEQ ID NO: 258) 5′-CGCAGACTCCGTGCGTGGCCGGTTCACC-3′ 271 61N 64R SDM F  (SEQ ID NO: 259) 5′-GGACATACTACGCAAACTCCGTGCGTGGCCGGTTCACC-3′ 567 +A rev (Flanks the 3′ end of the dAb Vh gene) (SEQ ID NO: 260) 5′-CCCTGGTCACCGTCTCGAGCGCGTAATAAGCGGCCGCAGATTA-3′ 21-23 Fwd (Flanks the 5′ end of the dAb Vh gene) (SEQ ID NO: 261) 5′-ATAAGGCCATGGCGGAGGTGCAGCTGTTGGAGTCTG-3′

To determine the effect of the mutations the dAbs were expressed in TB ONEX™ supplemented with 100 ug/ml carbenicillin and antifoam (Sigma) at 30° C. for 72 hours with shaking at 250 rpm. The cultures were centrifuged (4,200 rpm for 40 minutes) and dAbs affinity purified using STREAMLINE™-protein A beads (Amersham Biosciences, GE HEALTHCARE™, UK) as before. Affinity and potency of the domain antibody variants were determined on the BIACORE™ T200 (data for preferred dAbs is shown in Example 9) and in the SBE-bla HEK 293T Cell Sensor assay (data for preferred dAbs is shown in Example 11).

The amino acid and nucleic acid sequences of DOM23h-439-25 (SEQ ID NO: 234) and DOM23h-271-123 (SEQ ID NO: 236) anti-human TGFRII dAbs modified to include the C-terminal Alanine and D61N, K64R or V48I mutations are given below:

DOM23h-439-40 (DOM23h-439-25 + C-terminal Alanine) Nucleic acid sequence (SEQ ID NO: 262) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTG TCTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGCGCG DOM23h-439-40 (DOM23h-439-25 + C-terminal Alanine) Amino acid sequence  (SEQ ID NO: 263) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFVSRIDSPGGRMADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSSA DOM23h-439-41 (DOM23h-439-25 + C-terminal Alanine + 48I) Nucleic acid sequence  (SEQ ID NO: 264) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTA TTTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGCGCG DOM23h-439-41 (DOM23h-439-25 + C-terminal Alanine + 48I) Amino acid sequence  (SEQ ID NO: 265) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFISRIDSPGGRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSSA DOM23h-439-42 (DOM23h-439-25 + C-terminal Alanine + 61N) Nucleic acid sequence  (SEQ ID NO: 266) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTG TCTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGCGCG DOM23h-439-42 (DOM23h-439-25 + C-terminal Alanine + 61N) Amino acid sequence (SEQ ID NO: 267) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFVSRIDSPGGRTYYANSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSSA DOM23h-439-43 (DOM23h-439-25 + C-terminal Alanine + 64R) Nucleic acid sequence (SEQ ID NO: 268) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTG TCTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAGACTCCGTGCGTGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGCGCG DOM23h-439-43 (DOM23h-439-25 + C-terminal Alanine + 64R) Amino acid sequence (SEQ ID NO: 269) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFVSRIDSPGGRTYYADSVRGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSSA DOM23h-439-44 (DOM23h-439-25 + C-terminal Alanine + 61N64R) Nucleic acid sequence (SEQ ID NO: 270) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTG TCTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAAACTCCGTGCGTGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGCGCG DOM23h-439-44 (DOM23h-439-25 + C-terminal Alanine + 61N64R) Amino acid sequence (SEQ ID NO: 271) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFVSRIDSPGGRTYYANSVRGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSSA DOM23h-271-130 (DOM23h-271-123 + C-terminal Alanine) Nucleic acid sequence (SEQ ID NO: 272) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCGGCGAGAAGTGGGCGAGGCGGTGGGATTTGGACTACTGGGGTCAGGGAACCCT GGTCACCGTCTCGAGCGCG DOM23h-271-130 (DOM23h-271-123 + C-terminal Alanine) Amino acid sequence (SEQ ID NO: 273) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPGEKWARRWDLDYWGQGTLVTVSSA DOM23h-271-131 (DOM23h-271-123 + C-terminal Alanine + 61N) Nucleic acid sequence (SEQ ID NO: 274) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCGGCGAGAAGTGGGCGAGGCGGTGGGATTTGGACTACTGGGGTCAGGGAACCCT GGTCACCGTCTCGAGCGCG DOM23h-271-131 (DOM23h-271-123 + C-terminal Alanine + 61N) Amino acid sequence (SEQ ID NO: 275) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYANSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPGEKWARRWDLDYWGQGTLVTVSSA DOM23h-271-132 (DOM23h-271-123 + C-terminal Alanine + 64R) Nucleic acid sequence (SEQ ID NO: 276) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAGACTCCGTGCGTGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCGGCGAGAAGTGGGCGAGGCGGTGGGATTTGGACTACTGGGGTCAGGGAACCCT GGTCACCGTCTCGAGCGCG DOM23h-271-132 (DOM23h-271-123 + C-terminal Alanine + 64R) Amino acid sequence (SEQ ID NO: 277) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYADSVRGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPGEKWARRWDLDYWGQGTLVTVSSA DOM23h-271-133 (DOM23h-271-123 + C-terminal Alanine + 61N64R) Nucleic acid sequence (SEQ ID NO: 278) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAAACTCCGTGCGTGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCGGCGAGAAGTGGGCGAGGCGGTGGGATTTGGACTACTGGGGTCAGGGAACCCT GGTCACCGTCTCGAGCGCG DOM23h-271-133 (DOM23h-271-123 + C-terminal Alanine + 61N64R) Amino acid sequence (SEQ ID NO: 279) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYANSVRGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPGEKWARRWDLDYWGQGTLVTVSSA DOM23h-271-134 (DOM23h-271-123 + C-terminal Alanine + 48I) Nucleic acid sequence (SEQ ID NO: 280) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGA TTTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCGGCGAGAAGTGGGCGAGGCGGTGGGATTTGGACTACTGGGGTCAGGGAACCCT GGTCACCGTCTCGAGCGCG DOM23h-271-134 (DOM23h-271-123 + C-terminal Alanine + 48I) Amino acid sequence (SEQ ID NO: 281) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWISAIEPIGHRTYYADSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKQAPGEKWARRWDLDYWGQGTLVTVSSA DOM23h-271-135 (DOM23h-271-129 + C-terminal Alanine) Nucleic acid sequence (SEQ ID NO: 282) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCAATCAGAGGTATGTTGCCCGGGGCCGCTTGGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGCGCG DOM23h-271-135 (DOM23h-271-129 + C-terminal Alanine) Amino acid sequence (SEQ ID NO: 283) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYADSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPNQRYVARGRLDYWGQGTLVTVSSA DOM23h-271-136 (DOM23h-271-129 + C-terminal Alanine + 61N) Nucleic acid sequence (SEQ ID NO: 284) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCAATCAGAGGTATGTTGCCCGGGGCCGCTTGGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGCGCG DOM23h-271-136 (DOM23h-271-129 + C-terminal Alanine + 61N) Amino acid sequence (SEQ ID NO: 285) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYANSVKGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPNQRYVARGRLDYWGQGTLVTVSSA DOM23h-271-137 (DOM23h-271-129 + C-terminal Alanine + 61N64R) Nucleic acid sequence (SEQ ID NO: 286) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATCCACCTTTACGGAGTATAGGATGTGGTGGGTCCGCCAGGCTCCGGGGAAGGGTCTCGAGTGGG TCTCAGCGATTGAGCCGATTGGTCATAGGACATACTACGCAAACTCCGTGCGTGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGATACCGCGGTATATTACT GTGCGAAACAGGCGCCCAATCAGAGGTATGTTGCCCGGGGCCGCTTGGACTACTGGGGTCAGGGAACCCTG GTCACCGTCTCGAGCGCG DOM23h-271-137 (DOM23h-271-129 + C-terminal Alanine + 61N64R) Amino acid sequence (SEQ ID NO: 287) EVQLLESGGGLVQPGGSLRLSCAASGSTFTEYRMWWVRQAPGKGLEWVSAIEPIGHRTYYANSVRGRFTISRD NSKNTLYLQMNSLRAEDTAVYYCAKQAPNQRYVARGRLDYWGQGTLVTVSSA DOM23h-439-47 (DOM23h-439-42 + 48I) Nucleic acid sequence  (SEQ ID NO: 288) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTA TTTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAAACTCCGTGAAGGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGCGCG DOM23h-439-47 (DOM23h-439-42 + 48I) Amino acid sequence  (SEQ ID NO: 289) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFISRIDSPGGRTYYANSVKGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSSA DOM23h-439-48 (DOM23h-439-44 + 48I) Nucleic acid sequence  (SEQ ID NO: 290) GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGCGTCTCTCCTGTGCAGC CTCCGGATTCACCTTTGGGACGGAGCAGATGTGGTGGGTCCGCCAGGCTCCAGGGAAGGGTCTAGAGTTTA TTTCACGTATTGATTCGCCTGGTGGGAGGACATACTACGCAAACTCCGTGCGTGGCCGGTTCACCATCTCCC GCGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGCGTGCCGAGGACACCGCGGTATATTACT GTGCGAAACGGCGACCCACGGGGGTGTCCGGGACGATGACTACTGGGGTCAGGGAACCCTGGTCACC GTCTCGAGCGCG DOM23h-439-48 (DOM23h-439-44 + 48I) Amino acid sequence  (SEQ ID NO: 291) EVQLLESGGGLVQPGGSLRLSCAASGFTFGTEQMWWVRQAPGKGLEFISRIDSPGGRTYYANSVRGRFTISRDN SKNTLYLQMNSLRAEDTAVYYCAKRRPTGVSGTFYDYWGQGTLVTVSSA

Example 9 Biacore Kinetic Analysis of Affinity Matured Domain Antibodies

Anti-human IgG was immobilised on a Biacore CM4 chip by primary amine coupling according to the manufacturer's instructions. Human TGF-β RII/Fc, cynomolgus TGF-β RII/Fc or human Fc fragment were captured on this surface. Domain antibodies were passed over the two captured receptors at 3 concentrations of 100, 10 and 1 nM (DOM23h-439 dAbs) or 100, 25 and 6.25 nM (DOM23h-271 dAbs). Only the 100 nM concentration of each dab was passed over human Fc fragment to confirm specificity of binding to the extracellular TGF-β RII domain. An injection of buffer over the captured antigen surface was used for double referencing. The captured surface was regenerated, after each domain antibody injection using 3M magnesium chloride solution; the regeneration removed the captured antigen but did not significantly affect the ability of the surface to capture antigen in a subsequent cycle. All runs were carried out at 25° C. using HBS-EP buffer as running buffer. Data were generated using the BIACORE™ T200 and fitted to the 1:1 binding model inherent to the software. Table 14 shows the binding kinetics of the dAbs tested. The DOM23h-271 dAbs and the DOM23h-439 lineages were run in separate experiments.

TABLE 14 Human TGFBRII Cyno TGFBRII sample Ka (M⁻¹ · s⁻¹) Kd (s⁻¹) KD (M) Ka (M⁻¹ · s⁻¹) Kd (s⁻¹) KD (M) DOM23h-271-123 2.89E+06 2.93E−04 1.02E−10 3.08E+06 3.20E−04 1.04E−10 DOM23h-271-129 5.55E+06 4.11E−04 7.41E−11 6.00E+06 4.27E−04 7.12E−11 DOM23h-271-130 2.51E+06 3.09E−04 1.23E−10 2.61E+06 3.24E−04 1.24E−10 DOM23h-271-131 6.26E+06 1.36E−04 2.17E−11 6.81E+06 1.44E−04 2.12E−11 DOM23h-271-132 1.22E+07 2.22E−04 1.82E−11 1.29E+07 2.38E−04 1.85E−11 DOM23h-271-133 8.47E+06 7.70E−05 9.09E−12 8.84E+06 8.71E−05 9.85E−12 DOM23h-439-25  7.77E+06 6.34E−04 8.17E−11 8.99E+06 2.73E−03 3.04E−10 DOM23h-439-35  2.26E+07 2.22E−04 9.83E−12 2.32E+07 6.75E−04 2.91E−11 DOM23h-439-40  1.34E+07 1.39E−03 1.04E−10 9.34E+06 3.42E−03 3.66E−10 DOM23h-439-41  1.81E+07 1.87E−04 1.04E−11 1.57E+07 5.22E−04 3.33E−11 DOM23h-439-42  4.09E+07 1.31E−04 3.20E−12 3.72E+07 4.10E−04 1.10E−11 DOM23h-439-43  8.83E+06 3.73E−04 4.23E−11 8.04E+06 1.20E−03 1.49E−10 DOM23h-439-44  2.39E+07 6.91E−05 2.89E−12 2.17E+07 1.47E−04 6.77E−12

Example 10 Biophysical Evaluation of Affinity Matured dAbs

The thermal stability of the dAbs was determined using Differential Scanning calorimeter (DSC). dAbs were dialysed overnight into PBS buffer and adjusted at a final concentration of 1 mg/ml. Dialysis buffer was used as a reference for all samples. DSC was performed using capillary cell microcalorimeter VP-DSC (GE healthcare/Microcal), at a heating rate of 180° C./hour. A typical scan range was from 20-90° C. for both the reference buffer and the protein sample. After each reference buffer and sample pair, the capillary cell was cleaned with a solution of 5% Decon (Fisher-Scientific) in water followed by PBS. Resulting data traces were analyzed using Origin 7.0 software. The DSC trace obtained from the reference buffer was subtracted from the sample trace. The precise molar concentration of the sample was entered into the data analysis routine to yield values for melting temperature (Tm), enthalpy (ΔH) and Vara Hoff enthalpy (ΔHv) values. Data were fitted to a non-2-state model. Best fit and dependencie values were obtained with either 1 or 2 transition events. On-set unfolding temperature was also determined by integrating from zero each sample thermogram. This value was then determined as the temperature at which 4 percentage of the sample was unfolded.

TABLE 14A On-set Protein name Apparent Tm (C.) temperature (C.) DOM23h-439-21 60.66 52.5 DOM23h-439-25 56.34 49 DOM23h-439-30 57.17 50 DOM23h-439-32 55.01 49 DOM23h-439-33 58.93 51 DOM23h-439-34 56.78 50 DOM23h-855-42 61.90 55 DOM23h-855-43 66.81 58.6 DOM23h-855-44 63.38 56 DOM23h-271-123 54.82 50.6 DOM23h-271-124 58.16 52 DOM23h-271-125 58.26 55 Analysis of Solution State by Size Exclusion Chromatography with Multi-Angle-LASER-Light Scattering (SEC-MALS)

To determine whether dAbs are monomeric or form higher order oligomers in solution, they were analyzed by SEC-MALLS (Size Exclusion Chromatography with Multi-Angle-LASER-Light-Scattering). Agilent 1100 series HPLC system with an autosampler and a UV detector (controlled by Empower software) was connected to Wyatt Mini Dawn Treos (Laser Light Scattering (LS) detector) and Wyatt Optilab rEX DRI (Differential Refractive Index (R1) detector). The detectors are connected in the following order -UV-LS-R1. Both RI and LS instruments operate at a wavelength of 658 nm; the UV signal is monitored at 280 nm and 220 nm. Domain antibodies (50 microliters injection at a concentration of 1 mg/mL in PBS) were separated according to their hydrodynamic properties by size exclusion chromatography using a TSK2000 column. The mobile phase was 0.2M NaCl, 0.1M NaPO4, 15% n-propanol. The intensity of the scattered light while protein passed through the detector was measured as a function of angle. This measurement taken together with the protein concentration determined using the RI detector allowed calculation of the molar mass using appropriate equations (integral part of the analysis software Astra v.5.3.4.14). The solution state as percentage monomer is shown in Table 15.

TABLE 15 Percentage monomer DOM23h-439-21 100% DOM23h-439-25 100% DOM23h-439-30 100% DOM23h-439-32 100% DOM23h-439-33 98.2%  DOM23h-439-34 97.7%  DOM23h-855-42 83.8%  DOM23h-855-43  83% DOM23h-855-44 64.7%  DOM23h-271-123 100% DOM23h-271-124 97.4% 

Example 11 TGFβ—RII Inhibition by Affinity Matured dAbs in the SBE-Bla HEK 293T Cell Sensor Assay

The assay was carried out exactly as outlined in example 4, assay h2.

The assay was performed multiple times to obtain an average and a range of values which are summarised in Table 16. The arithmetic mean IC50 was calculated using log IC50s, and the range was calculated by adding and subtracting the log standard deviation from the mean IC50, and then transforming back to IC50. The assay QC parameters were met; the robust Z factors were greater than 0.4 and the TGF-β EC80 was within 6 fold of the concentration added to the assay. The results are shown in Table 16.

TABLE 16 Cell Functional assay data for human specific clones plus VH Dummy dAb. IC50 nM IC50 range Mean (+/− log SD) n DOM23h-271-123 18.3  9.8-34.1 11 DOM23h-271-129 22.7 16.6-31.1 4 DOM23h-271-130 37.0 15.1-90.7 4 DOM23h-271-131 6.3 4.5-8.9 4 DOM23h-271-132 21.0 16.1-27.5 4 DOM23h-271-133 2.4 1.5-3.8 4 DOM23h-439-25 4.0  1.1-14.7 17 DOM23h-439-35 0.5 0.4-0.7 3 DOM23h-439-37 0.7 0.2-2.2 4 DOM23h-439-40 14 10.8-18.8 6 DOM23h-439-41 1.7 1.3-2.3 4 DOM23h-439-42 0.7 0.2-2.7 8 DOM23h-439-43 1.0 0.7-1.4 4 DOM23h-439-44 1.3 0.5-3.3 6 VHDummy2 >25119 25119 13

Sequence Concordance Table

SEQ ID NO DOM number Description 1 DOM23h-802 amino acid sequence - naive clone 2 DOM23h-803 amino acid sequence - naive clone 3 DOM23h-813 amino acid sequence - naive clone 4 DOM23h-815 amino acid sequence - naive clone 5 DOM23h-828 amino acid sequence - naive clone 6 DOM23h-830 amino acid sequence - naive clone 7 DOM23h-831 amino acid sequence - naive clone 8 DOM23h-840 amino acid sequence - naive clone 9 DOM23h-842 amino acid sequence - naive clone 10 DOM23h-843 amino acid sequence - naive clone 11 DOM23h-850 amino acid sequence - naive clone 12 DOM23h-854 amino acid sequence - naive clone 13 DOM23h-855 amino acid sequence - naive clone 14 DOM23h-865 amino acid sequence - naive clone 15 DOM23h-866 amino acid sequence - naive clone 16 DOM23h-874 amino acid sequence - naive clone 17 DOM23h-883 amino acid sequence - naive clone 18 DOM23h-903 amino acid sequence - naive clone 19 DOM23m-4 amino acid sequence - naive clone 20 DOM23m-29 amino acid sequence - naive clone 21 DOM23m-32 amino acid sequence - naive clone 22 DOM23m-62 amino acid sequence - naive clone 23 DOM23m-71 amino acid sequence - naive clone 24 DOM23m-72 amino acid sequence - naive clone 25 DOM23m-81 amino acid sequence - naive clone 26 DOM23m-99 amino acid sequence - naive clone 27 DOM23m-101 amino acid sequence - naive clone 28 DOM23m-352 amino acid sequence - naive clone 29 DOM23h-271-21 amino acid sequence - affinity matured 30 DOM23h-271-22 amino acid sequence - affinity matured 31 DOM23h-271-27 amino acid sequence - affinity matured 32 DOM23h-271-101 amino acid sequence - affinity matured 33 DOM23h-271-102 amino acid sequence - affinity matured 34 DOM23h-271-105 amino acid sequence - affinity matured 35 DOM23h-271-106 amino acid sequence - affinity matured 36 DOM23h-271-114 amino acid sequence - affinity matured 37 DOM23h-271-39 amino acid sequence - affinity matured plus D61R K64D mutation 38 DOM23h-271-40 amino acid sequence - affinity matured plus D61R K64F mutation 39 DOM23h-802 nucleic acid sequence - naive clone 40 DOM23h-803 nucleic acid sequence - naive clone 41 DOM23h-813 nucleic acid sequence - naive clone 42 DOM23h-815 nucleic acid sequence - naive clone 43 DOM23h-828 nucleic acid sequence - naive clone 44 DOM23h-830 nucleic acid sequence - naive clone 45 DOM23h-831 nucleic acid sequence - naive clone 46 DOM23h-840 nucleic acid sequence - naive clone 47 DOM23h-842 nucleic acid sequence - naive clone 48 DOM23h-843 nucleic acid sequence - naive clone 49 DOM23h-850 nucleic acid sequence - naive clone 50 DOM23h-854 nucleic acid sequence - naive clone 51 DOM23h-855 nucleic acid sequence - naive clone 52 DOM23h-865 nucleic acid sequence - naive clone 53 DOM23h-866 nucleic acid sequence - naive clone 54 DOM23h-874 nucleic acid sequence - naive clone 55 DOM23h-883 nucleic acid sequence - naive clone 56 DOM23h-903 nucleic acid sequence - naive clone 57 DOM23m-4 nucleic acid sequence - naive clone 58 DOM23m-29 nucleic acid sequence - naive clone 59 DOM23m-32 nucleic acid sequence - naive clone 60 DOM23m-62 nucleic acid sequence - naive clone 61 DOM23m-71 nucleic acid sequence - naive clone 62 DOM23m-72 nucleic acid sequence - naive clone 63 DOM23m-81 nucleic acid sequence - naive clone 64 DOM23m-99 nucleic acid sequence - naive clone 65 DOM23m-101 nucleic acid sequence - naive clone 66 DOM23m-352 nucleic acid sequence - naive clone 67 DOM23h-271-21 nucleic acid sequence - affinity matured 68 DOM23h-271-22 nucleic acid sequence - affinity matured 69 DOM23h-271-27 nucleic acid sequence - affinity matured 70 DOM23h-271-101 nucleic acid sequence - affinity matured 71 DOM23h-271-102 nucleic acid sequence - affinity matured 72 DOM23h-271-105 nucleic acid sequence - affinity matured 73 DOM23h-271-106 nucleic acid sequence - affinity matured 74 DOM23h-271-114 nucleic acid sequence - affinity matured 75 DOM23h-271-39 nucleic acid sequence - affinity matured plus D61R K64D mutation 76 DOM23h-271-40 nucleic acid sequence - affinity matured plus D61R K64F mutation 77 DOM23h-802 CDR1 113 . . . CDR2 149 . . . CDR3 78 DOM23h-803 CDR1 114 . . . CDR2 150 . . . CDR3 79 DOM23h-813 CDR1 115 . . . CDR2 151 . . . CDR3 80 DOM23h-815 CDR1 116 . . . CDR2 152 . . . CDR3 81 DOM23h-828 CDR1 117 . . . CDR2 153 . . . CDR3 82 DOM23h-830 CDR1 118 . . . CDR2 154 . . . CDR3 83 DOM23h-831 CDR1 119 . . . CDR2 155 . . . CDR3 84 DOM23h-840 CDR1 120 . . . CDR2 156 . . . CDR3 85 DOM23h-842 CDR1 121 . . . CDR2 157 . . . CDR3 86 DOM23h-843 CDR1 122 . . . CDR2 158 . . . CDR3 87 DOM23h-850 CDR1 123 . . . CDR2 159 . . . CDR3 88 DOM23h-854 CDR1 124 . . . CDR2 160 . . . CDR3 89 DOM23h-855 CDR1 125 . . . CDR2 161 . . . CDR3 90 DOM23h-865 CDR1 126 . . . CDR2 162 . . . CDR3 91 DOM23h-866 CDR1 127 . . . CDR2 163 . . . CDR3 92 DOM23h-874 CDR1 128 . . . CDR2 164 . . . CDR3 93 DOM23h-883 CDR1 129 . . . CDR2 165 . . . CDR3 94 DOM23h-903 CDR1 130 . . . CDR2 166 . . . CDR3 95 DOM23m-4 CDR1 131 . . . CDR2 167 . . . CDR3 96 DOM23m-29 CDR1 132 . . . CDR2 168 . . . CDR3 97 DOM23m-32 CDR1 133 . . . CDR2 169 . . . CDR3 98 DOM23m-62 CDR1 134 . . . CDR2 170 . . . CDR3 99 DOM23m-71 CDR1 135 . . . CDR2 171 . . . CDR3 100 DOM23m-72 CDR1 136 . . . CDR2 172 . . . CDR3 101 DOM23m-81 CDR1 137 . . . CDR2 173 . . . CDR3 102 DOM23m-99 CDR1 138 . . . CDR2 174 . . . CDR3 103 DOM23m-101 CDR1 139 . . . CDR2 175 . . . CDR3 104 DOM23m-352 CDR1 140 . . . CDR2 176 . . . CDR3 105 DOM23h-271-21 CDR1 141 . . . CDR2 177 . . . CDR3 106 DOM23h-271-22 CDR1 142 . . . CDR2 178 . . . CDR3 107 DOM23h-271-27 CDR1 143 . . . CDR2 179 . . . CDR3 108 DOM23h-271-101 CDR1 144 . . . CDR2 180 . . . CDR3 109 DOM23h-271-102 CDR1 145 . . . CDR2 181 . . . CDR3 110 DOM23h-271-105 CDR1 146 . . . CDR2 182 . . . CDR3 111 DOM23h-271-106 CDR1 147 . . . CDR2 183 . . . CDR3 112 DOM23h-271-114 CDR1 148 . . . CDR2 184 . . . CDR3 185 DOM008 primer 186 DOM009 primer 187 DOM172 primer 188 DOM173 primer 189 271-7R1deg CDR1 primer 190 271-7R2deg CDR2 primer 191 271-7R3deg CDR3 primer 192 PE008 primer 193 271-6164 R primer 194 271-6164 deg-F primer 195 AS1309 primer 196 271-6164 NR-F primer 197 DOM57 primer 198 DOM6 primer 199 DOM23h-271 amino acid sequence - naive clone 200 DOM23h-271 nucleic acid sequence - naive clone 201 DOM23h-271-7 amino acid sequence - naive clone 202 DOM23h-271-7 nucleic acid sequence - naive clone 203 DOM23h-855-21 nucleic acid sequence - test matured clone 204 DOM23h-855-21 amino acid sequence - test matured clone 205 DOM23h-843-13 nucleic acid sequence - test matured clone 206 DOM23h-843-13 amino acid sequence - test matured clone 207 DOM23h-439-20 nucleic acid sequence - test matured clone 208 DOM23h-439-20 amino acid sequence - test matured clone 209 PEP-26-F Primer 210 PelB NcoVh Primer 211 PEP011 Primer 212 DOM-271-50 nucleic acid sequence - CDR-directed affinity matured clone 213 DOM-271-50 amino acid sequence - CDR-directed affinity matured clone 214 DOM-271-50 amino acid sequence - CDR-directed affinity matured clone (*duplicate of 213 above*) 215 PEP044 Primer 216 23h-439-20 CDRH1 Primer 217 23h-439-20 CDRH2 Primer 218 23h-439-20 CDRH3 Primer 219 23h-843-13 CDRH1 Primer 220 23h-843-13 CDRH2 Primer 221 23h-843-13 CDRH3 Primer 222 23h-855-21 CDRH1 Primer 223 23h-855-21 CDRH2 Primer 224 23h-855-21 CDRH3 Primer 225 H1-271-43 R Primer 226 H2p1-271-43 F Primer 227 H2p2-271-43 F Primer 228 H3p1-271-43 F Primer 229 H3p2-271-43 F Primer 230 H3p3-271-43 F Primer 231 PEP011VHStopNotIR Primer 232 Nco1 VH F Primer 233 DOM23h-439-25 nucleic acid sequence - CDR-directed affinity matured clone 234 DOM23h-439-25 amino acid sequence - CDR-directed affinity matured clone 235 DOM23h-271-123 nucleic acid sequence - CDR-directed affinity matured clone 236 DOM23h-271-123 amino acid sequence - CDR-directed affinity matured clone 237 DOM23h-439-35 nucleic acid sequence - CDR-directed affinity matured clone 238 DOM23h-439-35 amino acid sequence - CDR-directed affinity matured clone 239 DOM23h-271-129 nucleic acid sequence - CDR-directed affinity matured clone 240 DOM23h-271-129 amino acid sequence - CDR-directed affinity matured clone 241 DOM23h-271-123 CDR1 242 DOM23h-271-123 CDR2 243 DOM23h-271-123 CDR3 244 DOM23h-271-129 CDR1 245 DOM23h-271-129 CDR2 246 DOM23h-271-129 CDR3 247 DOM23h-439-25 CDR1 248 DOM23h-439-25 CDR2 249 DOM23h-439-25 CDR3 250 DOM23h-439-35 CDR1 251 DOM23h-439-35 CDR2 252 DOM23h-439-35 CDR3 253 439 48I SDM F Primer 254 439 61N SDM F Primer 255 439 64R SDM F Primer 256 439 61N 64R SDM F Primer 257 271 61N SDM F Primer 258 271 64R SDM F Primer 259 271 61N 64R SDM F Primer 260 567 +A rev Primer 261 21-23 Fwd Primer 262 DOM23h-439-40 nucleic acid sequence - CDR-directed affinity matured clone 263 DOM23h-439-40 amino acid sequence - CDR-directed affinity matured clone 264 DOM23h-439-41 nucleic acid sequence - CDR-directed affinity matured clone 265 DOM23h-439-41 amino acid sequence - CDR-directed affinity matured clone 266 DOM23h-439-42 nucleic acid sequence - CDR-directed affinity matured clone 267 DOM23h-439-42 amino acid sequence - CDR-directed affinity matured clone 268 DOM23h-439-43 nucleic acid sequence - CDR-directed affinity matured clone 269 DOM23h-439-43 amino acid sequence - CDR-directed affinity matured clone 270 DOM23h-439-44 nucleic acid sequence - CDR-directed affinity matured clone 271 DOM23h-439-44 amino acid sequence - CDR-directed affinity matured clone 272 DOM23h-271-130 nucleic acid sequence - CDR-directed affinity matured clone 273 DOM23h-271-130 amino acid sequence - CDR-directed affinity matured clone 274 DOM23h-271-131 nucleic acid sequence - CDR-directed affinity matured clone 275 DOM23h-271-131 amino acid sequence - CDR-directed affinity matured clone 276 DOM23h-271-132 nucleic acid sequence - CDR-directed affinity matured clone 277 DOM23h-271-132 amino acid sequence - CDR-directed affinity matured clone 278 DOM23h-271-133 nucleic acid sequence - CDR-directed affinity matured clone 279 DOM23h-271-133 amino acid sequence - CDR-directed affinity matured clone 280 DOM23h-271-134 nucleic acid sequence - CDR-directed affinity matured clone 281 DOM23h-271-134 amino acid sequence - CDR-directed affinity matured clone 282 DOM23h-271-135 nucleic acid sequence - CDR-directed affinity matured clone 283 DOM23h-271-135 amino acid sequence - CDR-directed affinity matured clone 284 DOM23h-271-136 nucleic acid sequence - CDR-directed affinity matured clone 285 DOM23h-271-136 amino acid sequence - CDR-directed affinity matured clone 286 DOM23h-271-137 nucleic acid sequence - CDR-directed affinity matured clone 287 DOM23h-271-137 amino acid sequence - CDR-directed affinity matured clone 288 DOM23h-439-47 nucleic acid sequence - DOM23h-439-42 + 48I 289 DOM23h-439-47 amino acid sequence - DOM23h-439-42 + 48I 290 DOM23h-439-48 nucleic acid sequence - DOM23h-439-44 + 48I 291 DOM23h-439-48 amino acid sequence - DOM23h-439-44 + 48I 

1. An anti-TGFbetaRII immunoglobulin single variable domain comprising an amino acid sequence as set forth in any one of SEQ ID NO:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289 and 291, having up to 5 amino acid substitutions, deletions or additions, in any combination.
 2. (canceled)
 3. An anti-TGFbetaRII immunoglobulin single variable domain as claimed in claim 1, wherein the said amino acid substitutions, deletions or additions are not within CDR3.
 4. An anti-TGFbetaRII immunoglobulin single variable domain as claimed in claim 1, wherein the said amino acid substitutions, deletions or additions are not within any of the CDRs.
 5. An anti-TGFbetaRII immunoglobulin single variable domain comprising of an amino acid sequence selected from the group consisting of SEQ ID NO:1-38, 204, 206, 208, 214, 234, 236, 238, 240, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289 and
 291. 6. (canceled)
 7. An anti-TGFbetaRII immunoglobulin single variable domain as claimed in claim 1, wherein the anti-TGFbetaRII immunoglobulin single variable domain further comprises a C-terminal alanine residue. 8.-12. (canceled)
 13. An anti-TGFbetaRII immunoglobulin single variable domain or polypeptide as claimed in claim 1, wherein said anti-TGFbetaRII immunoglobulin single variable domain or polypeptide binds to human TGFbetaRII.
 14. An anti-TGFbeta RII immunoglobulin single variable domain or polypeptide as claimed in claim 13, wherein said anti-TGFbetaRII immunoglobulin single variable domain or polypeptide also binds to mouse TGFbetaRII and/or cyno TGFbetaRII.
 15. An immunoglobulin single variable domain or polypeptide as claimed in claim 1, wherein said immunoglobulin single variable domain or polypeptide neutralises TGFbeta activity.
 16. An immunoglobulin single variable domain or polypeptide as claimed in claim 1, wherein said immunoglobulin single variable domain or polypeptide inhibits binding of TGFbeta to TGFbetaRII.
 17. An isolated nucleic acid encoding an anti-TGFbetaRII immunoglobulin single variable domain or polypeptide as claimed in claim
 1. 18. An isolated nucleic acid molecule as claimed in claim 17, comprising at least one nucleic acid molecule selected from the group of: SEQ ID NOs: 39-76, 203, 205, 207, 212, 233, 235, 237, 239, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288,
 290. 19. A vector comprising a nucleic acid molecule as claimed in claim
 17. 20. A host cell comprising a nucleic acid or a vector as claimed in claim
 17. 21. (canceled)
 22. (canceled)
 23. A pharmaceutical composition comprising an anti-TGFbetaRII immunoglobulin single variable domain or polypeptide as claimed in claim
 1. 24. A pharmaceutical composition as claimed in claim 23 for treatment of a disease associated with TGFbeta signaling.
 25. An anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or pharmaceutical composition as claimed in claim 24, wherein the disease associated with TGFbeta signalling is selected from the group of: tissue fibrosis, such as pulmonary fibrosis, including idiopathic pulmonary fibrosis; liver fibrosis, including cirrhosis and chronic hepatitis; rheumatoid arthritis; ocular disorders; fibrosis of the skin, including keloid of skin, and Dupuytren's Contracture; kidney fibrosis such as nephritis and nephrosclerosis, wound healing; and a vascular condition, such as restenosis.
 26. An anti-TGFbetaRII immunoglobulin single variable domain, polypeptide or pharmaceutical composition as claimed in claim 22, for use in treating tissue fibrosis or for use in wound healing and/or scarring reduction.
 27. An anti-TGFbetaRII immunoglobulin single variable domain as claimed in claim 22, wherein the disease is keloid disease or Dupuytren's Contracture.
 28. (canceled)
 29. A method of treating and/or preventing a TGFbeta-mediated condition in a human patient, the method comprising the step of: administering a composition comprising an anti-TGFbetaRII immunoglobulin single variable domain or polypeptide as claimed in claim 1 to the human patient.
 30. (canceled)
 31. (canceled) 